Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JZY03_RS18085 Genome accession   NZ_CP071178
Coordinates   3866187..3867167 (-) Length   326 a.a.
NCBI ID   WP_063943590.1    Uniprot ID   -
Organism   Enterobacter kobei strain CDL29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3861187..3872167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JZY03_RS18055 (JZY03_18015) - 3862076..3862573 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  JZY03_RS18060 (JZY03_18020) endA 3862668..3863375 (+) 708 WP_045134810.1 deoxyribonuclease I -
  JZY03_RS18065 (JZY03_18025) rsmE 3863427..3864158 (+) 732 WP_023337758.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JZY03_RS18070 (JZY03_18030) gshB 3864178..3865125 (+) 948 WP_023331278.1 glutathione synthase -
  JZY03_RS18075 (JZY03_18035) - 3865200..3865760 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  JZY03_RS18080 (JZY03_18040) ruvX 3865760..3866176 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  JZY03_RS18085 (JZY03_18045) pilT 3866187..3867167 (-) 981 WP_063943590.1 type IV pilus twitching motility protein PilT Machinery gene
  JZY03_RS18090 (JZY03_18050) - 3867185..3867886 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  JZY03_RS18095 (JZY03_18055) - 3867908..3868474 (+) 567 WP_014885192.1 YggT family protein -
  JZY03_RS18100 (JZY03_18060) yggU 3868471..3868767 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  JZY03_RS18105 (JZY03_18065) - 3868771..3869364 (+) 594 WP_063943588.1 XTP/dITP diphosphatase -
  JZY03_RS18110 (JZY03_18070) hemW 3869357..3870499 (+) 1143 WP_014885195.1 radical SAM family heme chaperone HemW -
  JZY03_RS18115 (JZY03_18075) - 3870563..3870901 (+) 339 WP_023331282.1 endonuclease domain-containing protein -
  JZY03_RS18120 (JZY03_18080) - 3870983..3871699 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  JZY03_RS18125 (JZY03_18085) - 3871756..3872082 (-) 327 WP_063943586.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35576.49 Da        Isoelectric Point: 6.2321

>NTDB_id=543503 JZY03_RS18085 WP_063943590.1 3866187..3867167(-) (pilT) [Enterobacter kobei strain CDL29]
MDVEEMVALSVNHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=543503 JZY03_RS18085 WP_063943590.1 3866187..3867167(-) (pilT) [Enterobacter kobei strain CDL29]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAATCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCAGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAAATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAGAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

45.748

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383