Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JY395_RS18440 Genome accession   NZ_CP071063
Coordinates   3929676..3930656 (-) Length   326 a.a.
NCBI ID   WP_109081757.1    Uniprot ID   -
Organism   Enterobacter mori strain 08-091     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3924676..3935656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JY395_RS18410 (JY395_18395) - 3925565..3926062 (+) 498 WP_010435446.1 SprT family zinc-dependent metalloprotease -
  JY395_RS18415 (JY395_18400) endA 3926157..3926864 (+) 708 WP_215230245.1 deoxyribonuclease I -
  JY395_RS18420 (JY395_18405) rsmE 3926916..3927647 (+) 732 WP_010435451.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JY395_RS18425 (JY395_18410) gshB 3927667..3928614 (+) 948 WP_215230246.1 glutathione synthase -
  JY395_RS18430 (JY395_18415) - 3928689..3929249 (+) 561 WP_010435456.1 YqgE/AlgH family protein -
  JY395_RS18435 (JY395_18420) ruvX 3929249..3929665 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  JY395_RS18440 (JY395_18425) pilT 3929676..3930656 (-) 981 WP_109081757.1 type IV pilus twitching motility protein PilT Machinery gene
  JY395_RS18445 (JY395_18430) - 3930674..3931375 (+) 702 WP_100166385.1 YggS family pyridoxal phosphate-dependent enzyme -
  JY395_RS18450 (JY395_18435) - 3931397..3931963 (+) 567 WP_010435467.1 YggT family protein -
  JY395_RS18455 (JY395_18440) yggU 3931960..3932256 (+) 297 WP_042714751.1 DUF167 family protein YggU -
  JY395_RS18460 (JY395_18445) - 3932260..3932853 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  JY395_RS18465 (JY395_18450) hemW 3932846..3933988 (+) 1143 WP_182945923.1 radical SAM family heme chaperone HemW -
  JY395_RS18470 (JY395_18455) - 3934052..3934414 (+) 363 WP_100166387.1 endonuclease domain-containing protein -
  JY395_RS18475 (JY395_18460) - 3934482..3935198 (-) 717 WP_100166388.1 DUF2884 domain-containing protein -
  JY395_RS18480 (JY395_18465) - 3935256..3935582 (-) 327 WP_010435485.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35626.67 Da        Isoelectric Point: 6.0399

>NTDB_id=542635 JY395_RS18440 WP_109081757.1 3929676..3930656(-) (pilT) [Enterobacter mori strain 08-091]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRG
SAFTHMKGVSIALRLLPLACPQLSALGVPRAIPELLSSESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSLSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=542635 JY395_RS18440 WP_109081757.1 3929676..3930656(-) (pilT) [Enterobacter mori strain 08-091]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCGGCCCCGTTTCCGGCCCCGGACGTTGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGACTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCATATGAAGGGGGTGTCGATCGCCTTGCGCCTGTTGCCGCTCGCGTGCCCGCAGCTTTCTGCACTGGG
TGTGCCCAGGGCTATCCCGGAACTGCTCTCCAGCGAAAGCGGGTTGATTCTGGTGACGGGGGCGACCGGTAGCGGAAAAT
CCACGACGCTCGCGGCGATGGTGGATTTTCTCAACCATCACACTGACGGCCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAGCGCTGCCTGATACAGCAGCGGGAGATTGGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
CAGTGCGCTGCGCGAAGATCCGGACGTTATTCTGTTGGGAGAGCTGCGCGACAGCGAAACCATCCGCCTTGCGCTGACGG
CGGCCGAAACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGCGCTTCGCAGGCGATTGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAACCAGCTGGCTGGCAGCCTTCGCGCTGTACTGGCGCAAAAACTGCTCCA
GGACGTGCAGGGCGGGCGCGTGGCGCTTTATGAACTGCTGGTGAACACCCCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AAACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTCGCGGAAAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38