Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JYG36_RS25925 Genome accession   NZ_CP071007
Coordinates   5662778..5663806 (-) Length   342 a.a.
NCBI ID   WP_093377194.1    Uniprot ID   -
Organism   Pseudomonas sp. SORT22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5657778..5668806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYG36_RS25905 (JYG36_25905) argS 5659004..5660740 (+) 1737 WP_045193990.1 arginine--tRNA ligase -
  JYG36_RS25910 (JYG36_25910) - 5660742..5661431 (+) 690 WP_038997644.1 SPOR domain-containing protein -
  JYG36_RS25915 (JYG36_25915) - 5661480..5661899 (-) 420 WP_045193957.1 TM2 domain-containing protein -
  JYG36_RS25920 (JYG36_25920) - 5662146..5662781 (+) 636 WP_045193958.1 C40 family peptidase -
  JYG36_RS25925 (JYG36_25925) pilT 5662778..5663806 (-) 1029 WP_093377194.1 type IV pilus twitching motility protein PilT Machinery gene
  JYG36_RS25930 (JYG36_25930) - 5663873..5664559 (+) 687 WP_213602719.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYG36_RS25935 (JYG36_25935) proC 5664598..5665416 (+) 819 WP_213602721.1 pyrroline-5-carboxylate reductase -
  JYG36_RS25940 (JYG36_25940) - 5665427..5666017 (+) 591 WP_045193962.1 YggT family protein -
  JYG36_RS25945 (JYG36_25945) - 5666143..5667282 (+) 1140 WP_045193963.1 homoserine O-acetyltransferase -
  JYG36_RS25950 (JYG36_25950) metW 5667290..5667910 (+) 621 WP_038997652.1 methionine biosynthesis protein MetW -
  JYG36_RS25955 (JYG36_25955) - 5667935..5668369 (+) 435 WP_045193964.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 342 a.a.        Molecular weight: 36559.03 Da        Isoelectric Point: 6.5040

>NTDB_id=542183 JYG36_RS25925 WP_093377194.1 5662778..5663806(-) (pilT) [Pseudomonas sp. SORT22]
MDVTELLALAVQAGASDLHLAAGEVPALRFGGRLRPLELAALTPQDMDEMLGQVMSAAQQKDFETHLDIDFAFAVPALAR
FRVNAFQQLHGAAAVLRVIPAQVSSLAELDLGDVFRQIAELSGGLVLVTGPTGSGKSTTLAALVDHLNRERALHIVTLED
PIEFIHSSQRSRISQREIGRHTRGFAQALRAALRQDPDVIMLGELRDPQSIRLALTAAETGHLVLATVHTGSAAKTIDRL
VDVFAAEEKQLVRAMLAESLQAVVSQVLVEKVGGGRLAAHEILLATPAVRNLVREGKVAQIYSAIQTGGAQGMQTLDACL
QGLVRQGLVSPESARERAPGRF

Nucleotide


Download         Length: 1029 bp        

>NTDB_id=542183 JYG36_RS25925 WP_093377194.1 5662778..5663806(-) (pilT) [Pseudomonas sp. SORT22]
ATGGATGTGACTGAACTGCTGGCGCTGGCTGTGCAAGCCGGTGCCAGTGACCTGCACCTGGCGGCGGGCGAGGTGCCGGC
GCTGCGTTTTGGGGGGCGTTTGCGGCCGCTGGAGCTGGCGGCGCTGACGCCGCAAGACATGGACGAAATGCTCGGGCAGG
TAATGTCCGCGGCGCAGCAAAAGGATTTTGAAACACATCTGGATATCGACTTTGCCTTTGCCGTGCCAGCATTGGCGCGC
TTTCGGGTCAATGCTTTCCAGCAACTGCATGGCGCCGCTGCAGTGCTGCGGGTGATTCCGGCGCAGGTCAGCAGCCTGGC
AGAACTCGACCTGGGGGATGTGTTTCGGCAAATTGCCGAGCTGTCGGGCGGGCTGGTGCTGGTTACCGGGCCCACAGGGT
CGGGCAAATCGACCACCCTGGCGGCTCTGGTCGATCACCTGAACCGTGAGCGGGCGCTGCACATCGTCACCCTCGAGGAC
CCCATCGAATTTATCCACAGCTCGCAGCGCAGCCGCATCAGCCAGCGCGAGATCGGCCGGCACACCCGGGGTTTTGCCCA
GGCCTTGCGCGCAGCATTACGTCAGGACCCGGATGTGATCATGCTCGGTGAACTGCGCGATCCGCAGAGCATTCGCCTGG
CCCTGACTGCAGCGGAAACCGGGCACCTGGTGCTGGCCACGGTGCATACCGGCTCGGCGGCTAAAACCATTGACCGCCTG
GTGGATGTGTTCGCCGCCGAAGAGAAGCAACTGGTACGCGCCATGCTCGCCGAGTCGCTGCAGGCAGTGGTCTCGCAGGT
TCTGGTGGAGAAGGTTGGCGGCGGCCGGCTGGCAGCCCACGAGATACTCCTGGCAACGCCTGCGGTGCGCAACCTGGTGC
GCGAGGGCAAGGTGGCGCAGATTTATTCGGCGATACAGACAGGCGGGGCACAGGGGATGCAGACCCTCGACGCGTGTCTG
CAGGGCCTGGTGCGTCAAGGGCTGGTCAGCCCTGAAAGCGCCCGGGAGCGGGCGCCGGGGCGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

59.884

100

0.602
  pilT Pseudomonas stutzeri DSM 10701

59.593

100

0.599
  pilT Acinetobacter nosocomialis M2

59.403

97.953

0.582
  pilT Acinetobacter baumannii D1279779

59.403

97.953

0.582
  pilT Acinetobacter baumannii strain A118

59.403

97.953

0.582
  pilT Acinetobacter baylyi ADP1

57.558

100

0.579
  pilT Neisseria meningitidis 8013

56.342

99.123

0.558
  pilT Neisseria gonorrhoeae MS11

56.047

99.123

0.556
  pilT Legionella pneumophila strain Lp02

54.252

99.708

0.541
  pilT Legionella pneumophila strain ERS1305867

54.252

99.708

0.541
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.006

98.538

0.532
  pilT Vibrio cholerae strain A1552

54.006

98.538

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

99.123

0.497
  pilU Pseudomonas stutzeri DSM 10701

38.806

97.953

0.38
  pilU Acinetobacter baylyi ADP1

37.243

99.708

0.371