Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JTM80_RS12250 Genome accession   NZ_CP070366
Coordinates   2981330..2982460 (-) Length   376 a.a.
NCBI ID   WP_172356240.1    Uniprot ID   -
Organism   Thermoleptolyngbya sp. PKUAC-SCTB121     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2976330..2987460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTM80_RS12230 - 2977816..2978253 (+) 438 WP_172356248.1 DUF4079 domain-containing protein -
  JTM80_RS12235 - 2978274..2978861 (+) 588 WP_205369504.1 hypothetical protein -
  JTM80_RS12240 - 2978914..2979519 (-) 606 WP_172356244.1 hypothetical protein -
  JTM80_RS12245 - 2979669..2980904 (-) 1236 WP_205369506.1 type II secretion system F family protein -
  JTM80_RS12250 pilT 2981330..2982460 (-) 1131 WP_172356240.1 type IV pilus twitching motility protein PilT Machinery gene
  JTM80_RS12255 pilB/pilB1 2982568..2984571 (-) 2004 WP_172356238.1 GspE/PulE family protein Machinery gene
  JTM80_RS12260 grpE 2985346..2986041 (+) 696 WP_172356236.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41144.46 Da        Isoelectric Point: 6.7592

>NTDB_id=539486 JTM80_RS12250 WP_172356240.1 2981330..2982460(-) (pilT) [Thermoleptolyngbya sp. PKUAC-SCTB121]
MELMIEDLMEQLIEMGGSDMHLSAGLPPYFRINGHLAPINDEPMTSEQCQRLIFSMLNNTQRKNLEQNWELDCSYGVKGL
ARFRVNVYKDRGTYAACLRALSSKIPSFEKLGLPDVVREMAEKPRGLILVTGPTGSGKTTTLAAIIDLINRTRAEHILTV
EDPIEFVYEPVKSLIHQRQLGEDTKSFANALKAALREDPDVILVGEMRDLETISLAISAAETGHLVMGTLHTSSASQTVD
RMIDVFPAERQQQVRVQLSNSLVAVFSQTLVPKKNPKPGEFGRAMAQEIMVVTPAIANLIREGKTAQIYSAIQTGGKLGM
VTLEKVLADLYKAGTISFESAMSKTSRPDELQRLIGGAMQQPAPGGARPGAPVGTR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=539486 JTM80_RS12250 WP_172356240.1 2981330..2982460(-) (pilT) [Thermoleptolyngbya sp. PKUAC-SCTB121]
ATGGAATTGATGATCGAAGACTTGATGGAGCAACTGATTGAGATGGGCGGGTCGGATATGCACCTGTCGGCTGGATTGCC
GCCCTACTTCCGCATCAATGGCCACCTCGCCCCCATCAACGACGAGCCGATGACCTCGGAGCAATGCCAGCGGCTGATCT
TCAGTATGCTTAACAACACCCAGCGAAAGAACCTGGAGCAAAACTGGGAACTGGACTGCTCCTACGGTGTGAAAGGTCTG
GCCCGCTTCCGGGTGAACGTGTACAAAGATCGGGGCACCTACGCCGCCTGTTTGCGGGCGCTGAGTTCCAAAATTCCTAG
CTTCGAGAAGCTAGGTCTGCCGGACGTGGTGCGCGAAATGGCGGAAAAGCCCAGAGGACTGATCCTGGTGACGGGGCCGA
CGGGTTCCGGAAAAACCACGACGCTGGCGGCCATCATCGACCTGATCAACCGCACCCGTGCAGAACACATTCTGACTGTG
GAAGACCCCATTGAATTTGTCTATGAACCTGTTAAAAGCTTGATCCACCAGCGGCAGCTTGGGGAAGACACCAAGAGCTT
TGCAAACGCGCTGAAGGCGGCTCTACGGGAAGACCCAGACGTGATTCTGGTGGGCGAGATGCGGGACCTGGAGACGATTT
CACTGGCGATCTCGGCGGCCGAAACTGGACACCTCGTGATGGGAACACTGCATACCAGTTCTGCTTCCCAGACCGTTGAC
CGGATGATCGACGTGTTCCCGGCTGAGCGTCAACAGCAGGTGCGGGTGCAGCTTTCCAACTCGCTGGTGGCAGTGTTTAG
CCAAACGCTGGTGCCTAAGAAGAATCCGAAGCCCGGTGAATTTGGGCGAGCGATGGCACAGGAAATCATGGTCGTTACTC
CGGCGATCGCCAACCTGATTCGCGAAGGCAAAACCGCCCAAATCTATTCCGCTATTCAAACGGGCGGCAAACTGGGCATG
GTCACGCTGGAGAAGGTGCTGGCTGACCTATACAAAGCGGGCACAATCTCGTTTGAATCGGCTATGTCGAAAACCTCTCG
CCCCGACGAACTCCAGCGGCTGATCGGCGGCGCAATGCAGCAACCCGCGCCGGGTGGTGCGCGTCCGGGTGCGCCCGTGG
GCACTCGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.314

92.287

0.492
  pilT Pseudomonas aeruginosa PAK

53.314

92.287

0.492
  pilT Legionella pneumophila strain Lp02

53.043

91.755

0.487
  pilT Legionella pneumophila strain ERS1305867

53.043

91.755

0.487
  pilT Acinetobacter baylyi ADP1

52.45

92.287

0.484
  pilT Acinetobacter baumannii strain A118

51.297

92.287

0.473
  pilT Acinetobacter baumannii D1279779

51.297

92.287

0.473
  pilT Acinetobacter nosocomialis M2

51.297

92.287

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.087

97.34

0.468
  pilT Neisseria gonorrhoeae MS11

50.442

90.16

0.455
  pilT Neisseria meningitidis 8013

50.442

90.16

0.455
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.118

90.426

0.444
  pilT Vibrio cholerae strain A1552

49.118

90.426

0.444
  pilU Pseudomonas stutzeri DSM 10701

39.601

93.351

0.37