Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   JTM80_RS12255 Genome accession   NZ_CP070366
Coordinates   2982568..2984571 (-) Length   667 a.a.
NCBI ID   WP_172356238.1    Uniprot ID   -
Organism   Thermoleptolyngbya sp. PKUAC-SCTB121     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2977568..2989571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTM80_RS12230 - 2977816..2978253 (+) 438 WP_172356248.1 DUF4079 domain-containing protein -
  JTM80_RS12235 - 2978274..2978861 (+) 588 WP_205369504.1 hypothetical protein -
  JTM80_RS12240 - 2978914..2979519 (-) 606 WP_172356244.1 hypothetical protein -
  JTM80_RS12245 - 2979669..2980904 (-) 1236 WP_205369506.1 type II secretion system F family protein -
  JTM80_RS12250 pilT 2981330..2982460 (-) 1131 WP_172356240.1 type IV pilus twitching motility protein PilT Machinery gene
  JTM80_RS12255 pilB/pilB1 2982568..2984571 (-) 2004 WP_172356238.1 GspE/PulE family protein Machinery gene
  JTM80_RS12260 grpE 2985346..2986041 (+) 696 WP_172356236.1 nucleotide exchange factor GrpE -
  JTM80_RS12265 dnaK 2986412..2988409 (+) 1998 WP_205369511.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74504.24 Da        Isoelectric Point: 4.7720

>NTDB_id=539487 JTM80_RS12255 WP_172356238.1 2982568..2984571(-) (pilB/pilB1) [Thermoleptolyngbya sp. PKUAC-SCTB121]
MANSSSQRRALVVQHNFSPFSNKLIQAGYVNNDQMQQALVESRKTGRPLTDVLEMITGQQLPPDLLRQYKKQQLFELKIL
YGVESLDPEINEISTAQVGQLIDSLIPVDLCRRYRLVPLSRTESEPPAVLIAMVDPDDLAAQDDLNRILRPQGLGLMRMV
ITVEDYQRIISQYLDEQVERQKQLEFQSKVDVKADLEGLDYLDLEEPPDDIEANLDAALQDADAAPVIALVNKILVKALQ
EGVSDIHIEPQEEHLRIRFRKDGVLREGLEPLPKKIVPAVTARFKIIADLDIAERRMPQDGRIRRIFDGRKVDFRVNTLP
SRYGEKVVLRILDNSATQLGLDKLITDPETLQIVQEMVSRPFGLILVTGPTGSGKTTTLYSALAERNSPGVNISTAEDPI
EYSLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIARLEEMGV
ESFMVSSSLIGVLAQRLMRRVCPDCRIPYSPSPEELARFGLSASREAEVTFYKANELSPDEIQSARAQNTLCPTCKGSGY
KGRCGVYEVMRVTEQIQTLITEGAPTERIKEVAVDEGMKTLLAYSLNLVREGSTTLEEVERVTFTDTGLEAELKAKRKSS
LTCRNCAAELRPEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=539487 JTM80_RS12255 WP_172356238.1 2982568..2984571(-) (pilB/pilB1) [Thermoleptolyngbya sp. PKUAC-SCTB121]
ATGGCTAACTCTTCATCCCAACGTCGTGCCCTTGTTGTCCAGCATAATTTTTCGCCCTTCAGCAACAAGCTCATCCAGGC
GGGCTACGTCAACAACGACCAAATGCAGCAAGCCCTGGTGGAAAGCCGCAAAACAGGGCGACCGCTGACGGATGTGTTGG
AGATGATTACAGGTCAGCAGTTGCCGCCTGACCTGCTGCGCCAATATAAGAAGCAACAGCTTTTTGAACTTAAAATTCTC
TACGGAGTTGAATCCCTCGATCCGGAGATCAACGAAATTTCGACTGCCCAGGTTGGGCAACTCATCGATAGTCTCATTCC
CGTCGATCTGTGCCGTCGCTATCGCCTTGTGCCTCTGTCGCGCACCGAAAGCGAACCGCCCGCAGTGCTGATCGCAATGG
TCGATCCCGATGATCTGGCTGCACAAGACGACCTCAACCGCATTCTGCGTCCGCAGGGTCTGGGGCTAATGCGGATGGTG
ATCACGGTTGAAGACTATCAGCGGATCATCTCCCAATATCTAGATGAGCAGGTCGAACGCCAGAAGCAGCTCGAATTTCA
GTCCAAGGTGGATGTGAAGGCAGATCTTGAAGGGCTGGATTACCTCGATCTGGAGGAACCACCCGACGATATCGAAGCCA
ACTTGGATGCTGCCCTTCAGGATGCCGACGCGGCCCCTGTGATCGCCTTGGTCAACAAAATTCTGGTGAAGGCGCTTCAG
GAAGGGGTTTCCGATATCCACATCGAGCCGCAGGAAGAACATCTCCGCATTCGCTTTCGCAAAGACGGCGTGCTGCGGGA
GGGACTGGAGCCGCTGCCCAAGAAAATCGTTCCGGCGGTCACCGCTCGCTTCAAAATCATTGCTGATCTAGACATTGCCG
AACGCCGGATGCCCCAGGACGGGCGCATTCGCCGGATTTTTGACGGGCGCAAGGTGGACTTCCGGGTGAACACGTTGCCC
AGTCGCTACGGCGAAAAGGTGGTGCTGCGGATTCTCGACAACTCCGCTACTCAGCTGGGGCTGGACAAACTGATCACCGA
CCCAGAAACGCTGCAAATTGTGCAGGAAATGGTATCGCGTCCCTTTGGTCTGATCCTGGTGACGGGGCCGACTGGATCGG
GTAAAACGACAACGCTCTATTCTGCCCTGGCGGAGCGCAATAGTCCCGGCGTGAACATCAGCACTGCCGAAGACCCGATT
GAATACTCCCTACCGGGGATTACCCAGGTGCAGGTGATCCGCGAAAAAGGGATGGACTTTGCCTCGATTCTGCGAGCCTT
TCTGCGGCAAGACCCGGACGTGATTCTGGTGGGTGAGACTCGGGATAAGGAGACGGCGAAGACGGCGATCGAGGCGGCAC
TGACGGGACACCTGGTGCTGACAACGCTGCACACGAATGACGCTGCGAGTGCGATCGCCCGTCTCGAAGAAATGGGCGTA
GAGTCCTTTATGGTGTCTAGTTCGCTGATTGGCGTGCTGGCTCAGCGCCTCATGCGCCGCGTCTGCCCCGACTGCCGTAT
TCCCTACAGCCCTAGCCCCGAAGAACTGGCCCGCTTTGGCCTGTCGGCTTCTCGCGAAGCAGAAGTGACCTTCTATAAGG
CCAACGAACTGTCACCTGACGAAATTCAGTCCGCCCGCGCCCAAAACACCCTCTGCCCCACCTGCAAGGGCAGTGGCTAC
AAAGGACGCTGCGGGGTATACGAAGTCATGCGTGTAACGGAGCAAATCCAGACTCTCATTACTGAAGGCGCACCCACCGA
GCGAATCAAGGAAGTGGCAGTAGACGAGGGCATGAAGACCTTGCTGGCCTATAGCCTAAACCTGGTGCGCGAGGGCAGCA
CAACGCTTGAAGAGGTAGAGCGCGTCACCTTTACCGATACGGGATTGGAGGCGGAACTCAAAGCCAAGCGCAAGAGTTCG
CTGACTTGCCGCAACTGTGCTGCTGAGCTACGCCCCGAATGGCTAGATTGTCCCTATTGCACTACCCCCCGCTTCCAGGA
TTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-178)

Type II/IV secretion system protein (T2SSE)

(233-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

68.477

99.4

0.681
  pilF Thermus thermophilus HB27

39.32

92.654

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.969

93.103

0.363