Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JRC46_RS03855 Genome accession   NZ_CP070284
Coordinates   796264..797244 (+) Length   326 a.a.
NCBI ID   WP_149451488.1    Uniprot ID   -
Organism   Escherichia albertii strain Sample 169     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 791264..802244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JRC46_RS03825 (JRC46_03825) - 791869..792876 (+) 1008 WP_000745183.1 DUF1202 family protein -
  JRC46_RS03830 (JRC46_03830) hemW 792941..794077 (-) 1137 WP_059218090.1 radical SAM family heme chaperone HemW -
  JRC46_RS03835 (JRC46_03835) - 794070..794663 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  JRC46_RS03840 (JRC46_03840) yggU 794671..794961 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  JRC46_RS03845 (JRC46_03845) yggT 794958..795524 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  JRC46_RS03850 (JRC46_03850) yggS 795542..796246 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  JRC46_RS03855 (JRC46_03855) pilT 796264..797244 (+) 981 WP_149451488.1 type IV pilus twitching motility protein PilT Machinery gene
  JRC46_RS03860 (JRC46_03860) ruvX 797428..797844 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  JRC46_RS03865 (JRC46_03865) - 797844..798407 (-) 564 WP_010336945.1 YqgE/AlgH family protein -
  JRC46_RS03870 (JRC46_03870) gshB 798516..799466 (-) 951 WP_000593300.1 glutathione synthase -
  JRC46_RS03875 (JRC46_03875) rsmE 799479..800210 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JRC46_RS03880 (JRC46_03880) endA 800290..800997 (-) 708 WP_002461298.1 deoxyribonuclease I -
  JRC46_RS03885 (JRC46_03885) - 801092..801589 (-) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36049.14 Da        Isoelectric Point: 5.5860

>NTDB_id=538953 JRC46_RS03855 WP_149451488.1 796264..797244(+) (pilT) [Escherichia albertii strain Sample 169]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPTQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=538953 JRC46_RS03855 WP_149451488.1 796264..797244(+) (pilT) [Escherichia albertii strain Sample 169]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGTCCGCAACTCGAACAACTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCAGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGACTCAGGAAAAAGATCCTGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.253

100

0.39