Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JQN60_RS04215 Genome accession   NZ_CP070222
Coordinates   866963..867943 (+) Length   326 a.a.
NCBI ID   WP_079797581.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica strain Se29     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 861963..872943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JQN60_RS04185 (JQN60_04185) - 862540..863547 (+) 1008 WP_079806496.1 DUF1202 family protein -
  JQN60_RS04190 (JQN60_04190) hemW 863639..864775 (-) 1137 WP_079806495.1 radical SAM family heme chaperone HemW -
  JQN60_RS04195 (JQN60_04195) - 864768..865361 (-) 594 WP_001174766.1 XTP/dITP diphosphatase -
  JQN60_RS04200 (JQN60_04200) yggU 865369..865659 (-) 291 WP_001277206.1 DUF167 family protein YggU -
  JQN60_RS04205 (JQN60_04205) - 865656..866222 (-) 567 WP_001094848.1 YggT family protein -
  JQN60_RS04210 (JQN60_04210) - 866241..866945 (-) 705 WP_046093010.1 YggS family pyridoxal phosphate-dependent enzyme -
  JQN60_RS04215 (JQN60_04215) pilT 866963..867943 (+) 981 WP_079797581.1 type IV pilus twitching motility protein PilT Machinery gene
  JQN60_RS04220 (JQN60_04220) - 868075..868761 (+) 687 WP_023176925.1 IclR family transcriptional regulator -
  JQN60_RS04225 (JQN60_04225) ruvX 868809..869225 (-) 417 WP_000099434.1 Holliday junction resolvase RuvX -
  JQN60_RS04230 (JQN60_04230) - 869225..869788 (-) 564 WP_001053170.1 YqgE/AlgH family protein -
  JQN60_RS04235 (JQN60_04235) gshB 870001..870948 (-) 948 WP_000593294.1 glutathione synthase -
  JQN60_RS04240 (JQN60_04240) rsmE 870968..871699 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JQN60_RS04245 (JQN60_04245) endA 871775..872482 (-) 708 WP_000286129.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36074.17 Da        Isoelectric Point: 7.2737

>NTDB_id=538694 JQN60_RS04215 WP_079797581.1 866963..867943(+) (pilT) [Salmonella enterica subsp. enterica strain Se29]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRRQGRMEIAPFIAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLDGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGSGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYASKRCLIQQREIGQHCATFAAGLRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERVVDS
FPAQEKEPVRSQLAGNLRAVLSQKLEADRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQSR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=538694 JQN60_RS04215 WP_079797581.1 866963..867943(+) (pilT) [Salmonella enterica subsp. enterica strain Se29]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAGACAAGGACGGATGGAGATCGCGCCTTTTATCGCGCCTGATGTCGACAGGCTTCTACTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCATGGCCAGCTTGATTTCGCCGTTTCACTGGACGGTACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAGCAGGGAACGTCGCTGGCGCTACGGCTTTTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCACCGATAGTACCAGCACTACTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCAGCGGGAAAT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATTCTGACGCTGGAAGATCCTATTGAG
TACCGTTACGCCAGCAAGCGTTGCCTGATACAGCAACGGGAAATCGGCCAGCATTGCGCCACTTTTGCAGCCGGGCTGCG
TTCGGCATTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGAGTAGTGGATAGT
TTCCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGCAACCTGCGCGCCGTACTATCACAAAAACTGGAAGC
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGCGAAGGTA
AACTCCATCAGTTGGCCCATGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATCTCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.283

97.546

0.442
  pilT Legionella pneumophila strain ERS1305867

45.283

97.546

0.442
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

39.812

97.853

0.39
  pilU Pseudomonas stutzeri DSM 10701

36.446

100

0.371