Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EAB7_RS10085 Genome accession   NC_018665
Coordinates   1918710..1919753 (-) Length   347 a.a.
NCBI ID   WP_014970916.1    Uniprot ID   A0A1S2W614
Organism   Exiguobacterium antarcticum B7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1913710..1924753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAB7_RS10065 (Eab7_1966) - 1914249..1915991 (-) 1743 WP_014970912.1 fimbrial assembly protein -
  EAB7_RS10070 (Eab7_1967) - 1916010..1916765 (-) 756 WP_014970913.1 prepilin peptidase -
  EAB7_RS10075 (Eab7_1968) - 1916822..1917349 (-) 528 WP_014970914.1 type II secretion system protein -
  EAB7_RS10080 (Eab7_1969) - 1917488..1918705 (-) 1218 WP_014970915.1 type II secretion system F family protein -
  EAB7_RS10085 (Eab7_1970) pilT 1918710..1919753 (-) 1044 WP_014970916.1 type IV pilus twitching motility protein PilT Machinery gene
  EAB7_RS10090 (Eab7_1971) - 1919750..1921414 (-) 1665 WP_014970917.1 GspE/PulE family protein -
  EAB7_RS10095 (Eab7_1972) - 1921423..1921737 (-) 315 WP_014970918.1 hypothetical protein -
  EAB7_RS10100 (Eab7_1973) - 1921762..1923141 (-) 1380 WP_014970919.1 G5 domain-containing protein -
  EAB7_RS10105 (Eab7_1974) - 1923317..1923841 (+) 525 WP_014970920.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EAB7_RS10110 (Eab7_1975) - 1923804..1924409 (+) 606 WP_026829955.1 PilW family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38146.97 Da        Isoelectric Point: 7.0664

>NTDB_id=53646 EAB7_RS10085 WP_014970916.1 1918710..1919753(-) (pilT) [Exiguobacterium antarcticum B7]
MSTMERAQIEDILRSSKEKEASDVHLTAGSPPVFRINGTLTPFGTDKMKPIIIEGFVRSLITEEMFLQLKQDRDIDFSFG
VTGVARYRVNCFYQRGALSMAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYKR
IITLEDPIEYLHSHQKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETISTAVTAAETGHLVFATVHTSTAA
STVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRTDGTGRVAALEIMVETPAVSNLIRTGKEYQLQSVLQTGKQYGM
QTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=53646 EAB7_RS10085 WP_014970916.1 1918710..1919753(-) (pilT) [Exiguobacterium antarcticum B7]
ATGAGTACGATGGAACGCGCCCAAATTGAAGATATCTTACGATCGTCGAAAGAAAAAGAAGCATCTGACGTTCATCTGAC
GGCGGGGTCACCGCCGGTCTTTCGGATTAACGGAACGCTGACACCGTTCGGAACAGACAAAATGAAACCGATCATCATCG
AAGGATTCGTTCGCTCCCTTATTACCGAGGAAATGTTTCTGCAGTTAAAACAGGATCGGGACATTGATTTTTCATTTGGG
GTGACGGGAGTGGCGCGGTATCGTGTTAATTGTTTTTATCAGCGCGGTGCCTTGTCGATGGCGTTTCGGACGATTCCGAC
CGAAGTCCCGACGCTTGAACAATTAGCGTTACCACCGGTCTTAAAACGTTTTCTGACGAAACCACATGGCTTGATTCTCG
TGACAGGTCCGACGGGCTCCGGGAAATCGACGACACTTGCCGCGATGATCAATGAAATCAACCAGACGATGTATAAACGC
ATCATCACGCTCGAAGATCCGATTGAGTATTTACATAGCCACCAGAAAAGTCTGATTGACCAACGGGAAATCGGCATCGA
TGCGCCGCGTTTTGCGACCGGCTTACGATCTGCCCTCCGGCAAGATCCGGATGTCATTTTACTTGGTGAGATGCGGGACC
TCGAGACGATTTCGACCGCGGTCACGGCAGCCGAGACCGGCCACTTGGTGTTTGCGACCGTCCATACGTCGACGGCGGCC
TCGACCGTCAGTCGAATCATCGATGTGTTCCCGTCGGAGCAGCAAGATCAAATTCGGGCGCAACTGGCGAATGTCTTAGC
CGGTGTCGTGTCGCAACGTTTGATGCCGCGAACAGACGGCACGGGACGTGTTGCCGCACTGGAAATCATGGTCGAAACAC
CTGCTGTCTCGAACTTGATTCGGACCGGGAAAGAGTATCAGCTTCAATCCGTCTTACAAACGGGAAAACAGTACGGAATG
CAGACGATGCAGATGGCATTACAAGACTTAGTAAGTCGTGGCTTGATCCCAGCATCTGCCGCGACACCTTACATATCGGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2W614

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.915

94.524

0.481
  pilT Neisseria gonorrhoeae MS11

49.701

96.254

0.478
  pilT Neisseria meningitidis 8013

49.701

96.254

0.478
  pilT Acinetobacter nosocomialis M2

48.955

96.542

0.473
  pilT Acinetobacter baumannii D1279779

48.657

96.542

0.47
  pilT Acinetobacter baumannii strain A118

48.657

96.542

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.394

95.101

0.47
  pilT Vibrio cholerae strain A1552

49.394

95.101

0.47
  pilT Pseudomonas aeruginosa PAK

48.649

95.965

0.467
  pilT Acinetobacter baylyi ADP1

48.476

94.524

0.458
  pilT Pseudomonas stutzeri DSM 10701

47.447

95.965

0.455
  pilT Legionella pneumophila strain Lp02

46.246

95.965

0.444
  pilT Legionella pneumophila strain ERS1305867

46.246

95.965

0.444
  pilU Pseudomonas stutzeri DSM 10701

41.033

94.813

0.389
  pilU Acinetobacter baylyi ADP1

39.403

96.542

0.38

Multiple sequence alignment