Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   E1N14_RS06250 Genome accession   NZ_CP068230
Coordinates   1391888..1393000 (+) Length   370 a.a.
NCBI ID   WP_062793318.1    Uniprot ID   -
Organism   Shewanella algae strain CECT-5071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1386888..1398000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1N14_RS06215 (E1N14_006220) rdgB 1386937..1387539 (-) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E1N14_RS06220 (E1N14_006225) - 1387799..1388233 (-) 435 WP_025009466.1 DUF4426 domain-containing protein -
  E1N14_RS06225 (E1N14_006230) yggU 1388296..1388583 (-) 288 WP_037436422.1 DUF167 family protein YggU -
  E1N14_RS06230 (E1N14_006235) - 1388583..1389134 (-) 552 WP_025009464.1 YggT family protein -
  E1N14_RS06235 (E1N14_006240) proC 1389158..1389976 (-) 819 WP_062793319.1 pyrroline-5-carboxylate reductase -
  E1N14_RS06240 (E1N14_006245) - 1390109..1390804 (-) 696 WP_025009462.1 YggS family pyridoxal phosphate-dependent enzyme -
  E1N14_RS06245 (E1N14_006250) pilT 1390841..1391878 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  E1N14_RS06250 (E1N14_006255) pilU 1391888..1393000 (+) 1113 WP_062793318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E1N14_RS06255 (E1N14_006260) - 1393125..1394234 (+) 1110 WP_062793317.1 hypothetical protein -
  E1N14_RS06260 (E1N14_006265) - 1394274..1394855 (+) 582 WP_081737044.1 glutathione peroxidase -
  E1N14_RS06265 (E1N14_006270) hemH 1394890..1395903 (+) 1014 WP_025009459.1 ferrochelatase -
  E1N14_RS06270 (E1N14_006275) - 1396075..1396779 (+) 705 WP_037436417.1 tryptophan 2,3-dioxygenase family protein -
  E1N14_RS06275 (E1N14_006280) - 1396820..1397950 (+) 1131 WP_062793316.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41041.12 Da        Isoelectric Point: 6.3709

>NTDB_id=528312 E1N14_RS06250 WP_062793318.1 1391888..1393000(+) (pilU) [Shewanella algae strain CECT-5071]
MDVRPFLKAMVERKASDLFITAAFPPSAKVDGELRPLAENAFTPAQSLEFVESLMTPEQKKEFHETRECNFAVGAKELGR
FRVSAFWQRESAGCVMRRIETKIPTVEQLYLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALIGYRNANARGHILTIED
PVEFVHQHGKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKVDGSGRRAAIEVLINTPRVASLIQKNELHVLKDTMAKSNEQGMQTFDQA
LFKLYCEGEISYADALHHADSPNDLRLMIKLHSNDTGNSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=528312 E1N14_RS06250 WP_062793318.1 1391888..1393000(+) (pilU) [Shewanella algae strain CECT-5071]
ATGGACGTCAGACCTTTTCTCAAGGCTATGGTGGAGCGCAAGGCCTCGGATCTCTTTATTACCGCTGCCTTTCCTCCCAG
TGCCAAGGTGGACGGTGAACTCAGGCCCTTGGCGGAAAACGCCTTTACCCCGGCGCAGTCGCTGGAGTTTGTTGAGTCTT
TGATGACGCCGGAGCAGAAAAAGGAGTTTCACGAGACCCGCGAATGTAACTTTGCCGTGGGCGCCAAAGAGCTGGGGCGT
TTCCGGGTCAGTGCCTTCTGGCAACGGGAGTCGGCGGGCTGCGTGATGCGGCGTATCGAAACCAAGATCCCTACTGTGGA
GCAGCTGTACCTGCCGCCCATTCTCAAAGACTTGGTAATGAGCAAGCGCGGATTGGTGATCATGGTCGGGGGAACCGGTA
CGGGTAAGTCCACCTCGCTGGCGGCACTTATCGGTTACCGTAATGCTAATGCTAGGGGACACATACTCACCATCGAAGAT
CCGGTGGAATTTGTTCACCAACACGGCAAGAGCATTATCACCCAGCGGGAAGTGGGTATAGATACCGAGTCGTTTGATGC
GGCGCTCAAGAGCTCATTGCGCCAGGCGCCGGACGTTATCCTTATCGGTGAGATCCGTACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGCCACTTGTGTATGGCAACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCACCAACAACTGCTATTTGACCTGTCGCTTAACTTGCGCGGTATTGTGGCCCAGCA
GTTGGTGCCCAAGGTGGACGGCAGCGGCCGGCGCGCCGCCATCGAAGTGCTGATCAACACCCCCAGGGTGGCCAGTCTTA
TTCAAAAGAACGAACTGCACGTGCTCAAGGATACCATGGCCAAGTCCAACGAGCAGGGGATGCAAACCTTCGACCAGGCG
TTGTTCAAGCTCTACTGTGAAGGGGAGATCAGCTACGCCGATGCACTGCATCATGCCGACTCGCCAAACGACCTGCGACT
CATGATTAAACTCCACAGCAATGACACCGGCAACTCAGGTTTCATGGAGGGAGTTACCCTGGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.995

100

0.622
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

59.659

95.135

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.994

89.459

0.376
  pilT Legionella pneumophila strain ERS1305867

41.994

89.459

0.376
  pilT Acinetobacter baumannii D1279779

40.643

92.432

0.376
  pilT Acinetobacter baumannii strain A118

40.643

92.432

0.376
  pilT Acinetobacter nosocomialis M2

40.643

92.432

0.376
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas stutzeri DSM 10701

38.841

93.243

0.362
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362