Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   E1N14_RS06245 Genome accession   NZ_CP068230
Coordinates   1390841..1391878 (+) Length   345 a.a.
NCBI ID   WP_025009461.1    Uniprot ID   A0A1S2TIJ8
Organism   Shewanella algae strain CECT-5071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1385841..1396878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1N14_RS06215 (E1N14_006220) rdgB 1386937..1387539 (-) 603 WP_025009467.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  E1N14_RS06220 (E1N14_006225) - 1387799..1388233 (-) 435 WP_025009466.1 DUF4426 domain-containing protein -
  E1N14_RS06225 (E1N14_006230) yggU 1388296..1388583 (-) 288 WP_037436422.1 DUF167 family protein YggU -
  E1N14_RS06230 (E1N14_006235) - 1388583..1389134 (-) 552 WP_025009464.1 YggT family protein -
  E1N14_RS06235 (E1N14_006240) proC 1389158..1389976 (-) 819 WP_062793319.1 pyrroline-5-carboxylate reductase -
  E1N14_RS06240 (E1N14_006245) - 1390109..1390804 (-) 696 WP_025009462.1 YggS family pyridoxal phosphate-dependent enzyme -
  E1N14_RS06245 (E1N14_006250) pilT 1390841..1391878 (+) 1038 WP_025009461.1 type IV pilus twitching motility protein PilT Machinery gene
  E1N14_RS06250 (E1N14_006255) pilU 1391888..1393000 (+) 1113 WP_062793318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  E1N14_RS06255 (E1N14_006260) - 1393125..1394234 (+) 1110 WP_062793317.1 hypothetical protein -
  E1N14_RS06260 (E1N14_006265) - 1394274..1394855 (+) 582 WP_081737044.1 glutathione peroxidase -
  E1N14_RS06265 (E1N14_006270) hemH 1394890..1395903 (+) 1014 WP_025009459.1 ferrochelatase -
  E1N14_RS06270 (E1N14_006275) - 1396075..1396779 (+) 705 WP_037436417.1 tryptophan 2,3-dioxygenase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38371.97 Da        Isoelectric Point: 6.8739

>NTDB_id=528311 E1N14_RS06245 WP_025009461.1 1390841..1391878(+) (pilT) [Shewanella algae strain CECT-5071]
MEITELLAFSVKHNASDLHLSAGVPPMIRVDGEVRKINVPALDHQGVHRLVYDIMNDKQRKDYEEFLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSDILTLEQLGAPEIFKKIASYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQSL
QNLVNRGLISREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=528311 E1N14_RS06245 WP_025009461.1 1390841..1391878(+) (pilT) [Shewanella algae strain CECT-5071]
ATGGAAATCACTGAATTATTGGCCTTTAGTGTAAAGCATAACGCTTCGGATCTGCACCTGTCGGCCGGTGTGCCTCCCAT
GATACGCGTCGACGGCGAGGTCAGAAAGATTAATGTCCCTGCGCTTGATCACCAAGGGGTTCACAGACTTGTCTACGACA
TAATGAATGACAAGCAGCGTAAGGATTATGAAGAGTTTCTGGAAATCGACTTTTCCTTTGAAGTTCCCAATCTGGCGCGT
TTTCGTGTCAATGCCTTCAATCAGTCCCGCGGCGCCGCCGCGGTTTTTCGTACAATTCCCAGCGACATTTTGACTTTGGA
ACAACTGGGCGCGCCGGAAATCTTCAAGAAAATCGCTTCATATCCTCGCGGCCTGGTGTTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACTACCCTGGCGGCGATGATAGATTACGTCAATGAGAACCGCCACGATCATATTCTCACCATTGAAGAC
CCTATTGAATTCGTGCACCAAAACAAGCAATGCCTTATCAACCAGCGGGAAGTGCACCGCCATACCCACAGCTTTAACGC
CGCCCTCAGAAGCGCCCTGCGTGAAGATCCGGATGTCATCCTGGTCGGTGAGATGCGGGATTTGGAAACCATACGCCTGG
CGATGACCGCGGCAGAAACCGGCCACCTGGTGTTCGGTACCTTGCACACCACCTCGGCGGCCAAGACCATTGACCGTGTG
GTTGACGTATTTCCCGCAGGCGAGAAAGACATGGTGCGTACCATGTTGTCTGAGTCGTTGCAGGCGGTTATCTCCCAGAC
TCTGATCAAGAAAGTTGGCGGCGGCCGGGTGGCGGCCCATGAGATCATGATGGGAACCCCGGCTATCCGAAACCTTATCC
GTGAGGACAAGGTGGCACAGATGTATTCTGCTATTCAAACCGGTATGGCCCATGGCATGCAAACCTTGGAGCAGAGCCTG
CAGAACCTGGTGAATCGCGGCTTGATCAGTCGGGAAGATGCCATGGCCAAGAGCTCCAACAAACAAGCGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S2TIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.871

99.13

0.812
  pilT Acinetobacter baumannii D1279779

81.871

99.13

0.812
  pilT Acinetobacter baumannii strain A118

81.871

99.13

0.812
  pilT Acinetobacter baylyi ADP1

78.426

99.42

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.971

100

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.681

0.733
  pilT Vibrio cholerae strain A1552

75.074

97.681

0.733
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Acinetobacter baylyi ADP1

40.059

97.681

0.391