Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JHW33_RS11970 Genome accession   NZ_CP067057
Coordinates   2606309..2607331 (-) Length   340 a.a.
NCBI ID   WP_134706675.1    Uniprot ID   -
Organism   Rahnella aceris strain ZF458     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2601309..2612331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW33_RS11940 (JHW33_11940) - 2602089..2602610 (+) 522 WP_037033267.1 SprT family zinc-dependent metalloprotease -
  JHW33_RS11945 (JHW33_11945) endA 2602704..2603417 (+) 714 WP_037033268.1 deoxyribonuclease I -
  JHW33_RS11950 (JHW33_11950) rsmE 2603505..2604236 (+) 732 WP_037033269.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JHW33_RS11955 (JHW33_11955) gshB 2604248..2605198 (+) 951 WP_013576826.1 glutathione synthase -
  JHW33_RS11960 (JHW33_11960) - 2605327..2605890 (+) 564 WP_013576827.1 YqgE/AlgH family protein -
  JHW33_RS11965 (JHW33_11965) ruvX 2605890..2606318 (+) 429 WP_013576828.1 Holliday junction resolvase RuvX -
  JHW33_RS11970 (JHW33_11970) pilT 2606309..2607331 (-) 1023 WP_134706675.1 type IV pilus twitching motility protein PilT Machinery gene
  JHW33_RS11975 (JHW33_11975) - 2607354..2608073 (+) 720 WP_013576830.1 YggS family pyridoxal phosphate-dependent enzyme -
  JHW33_RS11980 (JHW33_11980) proC 2608146..2608967 (+) 822 WP_013576831.1 pyrroline-5-carboxylate reductase -
  JHW33_RS11985 (JHW33_11985) - 2609054..2609608 (+) 555 WP_013576832.1 YggT family protein -
  JHW33_RS11990 (JHW33_11990) yggU 2609593..2609898 (+) 306 WP_013576833.1 DUF167 family protein YggU -
  JHW33_RS11995 (JHW33_11995) - 2609920..2610528 (+) 609 WP_112150890.1 XTP/dITP diphosphatase -
  JHW33_RS12000 (JHW33_12000) hemW 2610521..2611657 (+) 1137 WP_200222800.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37020.51 Da        Isoelectric Point: 6.5597

>NTDB_id=524725 JHW33_RS11970 WP_134706675.1 2606309..2607331(-) (pilT) [Rahnella aceris strain ZF458]
MDIDGLVGRSVKQNASDLHLCTGYPPVLRIDGELCRCEDLPVVEASQMLGLIHGWLDATEQHTLQLAGQVDKALTLGDGV
RMRLNIFQQLHGLSAALRPVPARCPELAALRVPDILQHVIMQEDGLILVTGATGSGKSTTLAALIDHLNRHAKRHIITLE
DPIELIHTGRQCLIQQRELGLHTRSFGEAVRGALREDPDVLLLGELRDTDSIRLALTAAETGHLVLATLHTRNATQAIDR
LVDVFPAEEKAFVRAQLAGSLRAVIAQKLAVTEEKDGRKAGRVALFEVLTSTPAVCNLIREGKTHQLADILQTGAQAGMQ
TFAQSRQQRQRAGLLAEDQD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=524725 JHW33_RS11970 WP_134706675.1 2606309..2607331(-) (pilT) [Rahnella aceris strain ZF458]
ATGGATATTGATGGATTAGTGGGACGTAGTGTAAAGCAAAATGCCTCCGATCTGCACCTTTGTACCGGCTATCCCCCTGT
GTTGCGCATTGACGGGGAATTATGTCGCTGTGAAGATCTGCCGGTGGTGGAGGCTTCGCAGATGCTGGGGCTGATCCATG
GGTGGCTGGATGCCACTGAACAACATACTTTGCAGCTGGCCGGACAGGTGGATAAAGCGCTGACATTGGGTGATGGCGTG
CGGATGCGGCTCAATATCTTTCAGCAACTGCACGGGTTGTCTGCGGCGCTGAGACCGGTGCCTGCCCGTTGCCCCGAGCT
GGCTGCATTGAGGGTGCCGGATATCTTGCAGCACGTCATTATGCAGGAAGACGGCCTGATTTTGGTCACCGGTGCGACGG
GCAGCGGGAAATCCACCACGCTGGCGGCGCTGATTGATCATCTCAACCGCCATGCGAAGCGCCATATTATTACGCTGGAA
GACCCCATCGAGCTTATCCATACTGGCAGGCAATGTCTGATTCAGCAGCGCGAACTCGGGCTGCATACGCGTTCATTTGG
CGAAGCGGTGCGCGGTGCTTTGCGTGAAGATCCGGATGTATTGTTACTGGGCGAACTGCGTGACACTGACAGTATCCGGC
TGGCGCTGACGGCGGCAGAAACCGGACATCTGGTTCTCGCGACACTGCATACGCGCAACGCCACACAGGCCATTGACCGG
CTGGTTGATGTGTTTCCGGCAGAAGAAAAAGCGTTTGTCCGCGCGCAACTGGCGGGCAGCCTGCGGGCCGTGATCGCGCA
AAAACTGGCGGTGACTGAAGAAAAGGATGGGAGAAAAGCGGGAAGGGTAGCGCTTTTTGAAGTGCTGACCTCCACACCGG
CGGTGTGCAATTTAATTCGTGAAGGGAAAACGCATCAGCTGGCGGATATCCTGCAAACCGGCGCGCAGGCCGGCATGCAG
ACGTTCGCACAAAGCCGTCAGCAACGGCAACGTGCGGGATTACTGGCGGAAGATCAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.305

96.471

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

96.471

0.485
  pilT Neisseria meningitidis 8013

47.041

99.412

0.468
  pilT Neisseria gonorrhoeae MS11

46.746

99.412

0.465
  pilT Acinetobacter baylyi ADP1

47.164

98.529

0.465
  pilT Pseudomonas stutzeri DSM 10701

45.723

99.706

0.456
  pilT Pseudomonas aeruginosa PAK

47.826

94.706

0.453
  pilT Acinetobacter baumannii strain A118

47.077

95.588

0.45
  pilT Acinetobacter nosocomialis M2

47.077

95.588

0.45
  pilT Acinetobacter baumannii D1279779

47.077

95.588

0.45
  pilT Legionella pneumophila strain ERS1305867

46.296

95.294

0.441
  pilT Legionella pneumophila strain Lp02

46.296

95.294

0.441
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

94.706

0.403
  pilU Vibrio cholerae strain A1552

37.994

96.765

0.368
  pilU Acinetobacter baylyi ADP1

37.015

98.529

0.365