Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ECENHK_RS18780 Genome accession   NC_018405
Coordinates   3977908..3978888 (-) Length   326 a.a.
NCBI ID   WP_014885190.1    Uniprot ID   -
Organism   Enterobacter kobei     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3972908..3983888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECENHK_RS18750 (ECENHK_18550) - 3973797..3974294 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  ECENHK_RS18755 (ECENHK_18555) endA 3974389..3975096 (+) 708 WP_014885187.1 deoxyribonuclease I -
  ECENHK_RS18760 (ECENHK_18560) rsmE 3975148..3975879 (+) 732 WP_014885188.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ECENHK_RS18765 (ECENHK_18565) gshB 3975899..3976846 (+) 948 WP_014885189.1 glutathione synthase -
  ECENHK_RS18770 (ECENHK_18570) - 3976921..3977481 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  ECENHK_RS18775 (ECENHK_18575) ruvX 3977481..3977897 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  ECENHK_RS18780 (ECENHK_18580) pilT 3977908..3978888 (-) 981 WP_014885190.1 type IV pilus twitching motility protein PilT Machinery gene
  ECENHK_RS18785 (ECENHK_18585) - 3978906..3979607 (+) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  ECENHK_RS18790 (ECENHK_18590) - 3979629..3980195 (+) 567 WP_014885192.1 YggT family protein -
  ECENHK_RS18795 (ECENHK_18595) yggU 3980192..3980488 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  ECENHK_RS18800 (ECENHK_18600) - 3980492..3981085 (+) 594 WP_014885194.1 XTP/dITP diphosphatase -
  ECENHK_RS18805 (ECENHK_18605) hemW 3981078..3982220 (+) 1143 WP_014885195.1 radical SAM family heme chaperone HemW -
  ECENHK_RS18810 (ECENHK_18610) - 3982284..3982646 (+) 363 WP_014885196.1 endonuclease domain-containing protein -
  ECENHK_RS18815 (ECENHK_18615) - 3982721..3983437 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  ECENHK_RS18820 (ECENHK_18620) - 3983494..3983820 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35574.57 Da        Isoelectric Point: 6.4114

>NTDB_id=52366 ECENHK_RS18780 WP_014885190.1 3977908..3978888(-) (pilT) [Enterobacter kobei]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=52366 ECENHK_RS18780 WP_014885190.1 3977908..3978888(-) (pilT) [Enterobacter kobei]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCTGATGTCGGGGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAAATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACGTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAGAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

46.334

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386

Multiple sequence alignment