Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JH277_RS05345 Genome accession   NZ_CP066932
Coordinates   1287610..1288008 (+) Length   132 a.a.
NCBI ID   WP_104570927.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 576     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1276046..1305934 1287610..1288008 within 0


Gene organization within MGE regions


Location: 1276046..1305934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH277_RS05290 (JH277_05270) - 1276046..1276192 (+) 147 WP_076054137.1 hypothetical protein -
  JH277_RS05295 (JH277_05275) - 1276376..1276771 (+) 396 WP_042594933.1 hypothetical protein -
  JH277_RS05300 (JH277_05280) - 1276862..1277254 (+) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  JH277_RS05305 (JH277_05285) glgX 1277788..1279917 (-) 2130 WP_274299339.1 glycogen debranching protein GlgX -
  JH277_RS05310 (JH277_05290) rimK 1280413..1281288 (+) 876 WP_076054142.1 30S ribosomal protein S6--L-glutamate ligase -
  JH277_RS05315 (JH277_05295) - 1281938..1282615 (+) 678 WP_003490678.1 response regulator transcription factor -
  JH277_RS05320 (JH277_05300) - 1282608..1283942 (+) 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
  JH277_RS05325 (JH277_05305) - 1284041..1284311 (-) 271 Protein_1034 SymE family type I addiction module toxin -
  JH277_RS05330 (JH277_05310) coaE 1284490..1285113 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  JH277_RS05335 (JH277_05315) - 1285127..1285990 (-) 864 WP_011038216.1 A24 family peptidase -
  JH277_RS05340 (JH277_05320) pilC 1285997..1287259 (-) 1263 WP_104570928.1 type II secretion system F family protein Machinery gene
  JH277_RS05345 (JH277_05325) pilA/pilAI 1287610..1288008 (+) 399 WP_104570927.1 pilin Machinery gene
  JH277_RS05350 (JH277_05330) - 1288109..1288531 (+) 423 WP_104570926.1 pilin -
  JH277_RS05355 (JH277_05335) - 1288590..1290410 (+) 1821 WP_147308815.1 hypothetical protein -
  JH277_RS05360 (JH277_05340) - 1290534..1291292 (+) 759 WP_274299340.1 class I SAM-dependent methyltransferase -
  JH277_RS05365 (JH277_05345) - 1291789..1292475 (+) 687 WP_258872788.1 glycosyltransferase family 9 protein -
  JH277_RS05370 (JH277_05350) - 1292478..1293320 (-) 843 WP_147308817.1 glycosyltransferase -
  JH277_RS05375 (JH277_05355) - 1293314..1294486 (-) 1173 WP_181920810.1 glycosyltransferase -
  JH277_RS05380 (JH277_05360) - 1294471..1295334 (-) 864 WP_116921534.1 glycosyltransferase -
  JH277_RS05385 (JH277_05365) - 1295384..1296235 (-) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  JH277_RS05390 (JH277_05370) pilB 1296391..1298124 (+) 1734 WP_274299341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH277_RS05395 (JH277_05375) - 1298307..1299044 (-) 738 WP_274299342.1 zeta toxin family protein -
  JH277_RS05400 (JH277_05380) - 1299044..1299400 (-) 357 WP_139328384.1 hypothetical protein -
  JH277_RS05405 (JH277_05385) pilR 1299598..1300992 (-) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  JH277_RS05410 (JH277_05390) - 1301201..1302811 (-) 1611 WP_070690016.1 HAMP domain-containing sensor histidine kinase -
  JH277_RS05415 (JH277_05395) sucC 1303045..1304214 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  JH277_RS05420 (JH277_05400) sucD 1304239..1305114 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH277_RS05425 (JH277_05405) - 1305218..1305628 (+) 411 WP_323475224.1 DNA-binding protein -
  JH277_RS05430 (JH277_05410) - 1305632..1305934 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13656.88 Da        Isoelectric Point: 8.8372

>NTDB_id=523336 JH277_RS05345 WP_104570927.1 1287610..1288008(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain 576]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQYEVLVNEGTVPTTAAQIGLTTPTARCAV
VVSTSGITCTLNGNANKIKGKTISLNRSSADGTWSCVTGGTMDAKYKPAGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=523336 JH277_RS05345 WP_104570927.1 1287610..1288008(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain 576]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTAGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
ACCGATGTATCAGGACTATGTTGCCAAGTCGCAGGCGACTGCCGGTCTGGCAGAAATCACTCCTGGCAAGACGCAGTATG
AAGTGTTGGTCAACGAAGGTACTGTTCCTACGACGGCCGCCCAGATTGGCTTGACAACGCCGACTGCGCGTTGCGCTGTT
GTCGTAAGCACCTCTGGCATTACATGCACCTTGAATGGCAACGCGAACAAGATTAAAGGCAAAACCATTAGCCTTAACCG
TAGTTCCGCAGATGGTACTTGGAGCTGTGTAACTGGCGGCACCATGGACGCGAAATACAAGCCCGCCGGCTGTTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

58.394

100

0.606
  pilA Acinetobacter baumannii strain A118

51.064

100

0.545
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.667

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.47
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.447
  pilA2 Legionella pneumophila str. Paris

43.066

100

0.447
  comP Acinetobacter baylyi ADP1

39.456

100

0.439
  pilA Vibrio cholerae strain A1552

39.583

100

0.432
  pilA Vibrio cholerae C6706

39.583

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.432
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.409
  pilE Neisseria gonorrhoeae MS11

34.416

100

0.402
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

94.697

0.394
  pilA Haemophilus influenzae Rd KW20

38.346

100

0.386