Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH277_RS05390 Genome accession   NZ_CP066932
Coordinates   1296391..1298124 (+) Length   577 a.a.
NCBI ID   WP_274299341.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 576     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1276046..1305934 1296391..1298124 within 0


Gene organization within MGE regions


Location: 1276046..1305934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH277_RS05290 (JH277_05270) - 1276046..1276192 (+) 147 WP_076054137.1 hypothetical protein -
  JH277_RS05295 (JH277_05275) - 1276376..1276771 (+) 396 WP_042594933.1 hypothetical protein -
  JH277_RS05300 (JH277_05280) - 1276862..1277254 (+) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  JH277_RS05305 (JH277_05285) glgX 1277788..1279917 (-) 2130 WP_274299339.1 glycogen debranching protein GlgX -
  JH277_RS05310 (JH277_05290) rimK 1280413..1281288 (+) 876 WP_076054142.1 30S ribosomal protein S6--L-glutamate ligase -
  JH277_RS05315 (JH277_05295) - 1281938..1282615 (+) 678 WP_003490678.1 response regulator transcription factor -
  JH277_RS05320 (JH277_05300) - 1282608..1283942 (+) 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
  JH277_RS05325 (JH277_05305) - 1284041..1284311 (-) 271 Protein_1034 SymE family type I addiction module toxin -
  JH277_RS05330 (JH277_05310) coaE 1284490..1285113 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  JH277_RS05335 (JH277_05315) - 1285127..1285990 (-) 864 WP_011038216.1 A24 family peptidase -
  JH277_RS05340 (JH277_05320) pilC 1285997..1287259 (-) 1263 WP_104570928.1 type II secretion system F family protein Machinery gene
  JH277_RS05345 (JH277_05325) pilA/pilAI 1287610..1288008 (+) 399 WP_104570927.1 pilin Machinery gene
  JH277_RS05350 (JH277_05330) - 1288109..1288531 (+) 423 WP_104570926.1 pilin -
  JH277_RS05355 (JH277_05335) - 1288590..1290410 (+) 1821 WP_147308815.1 hypothetical protein -
  JH277_RS05360 (JH277_05340) - 1290534..1291292 (+) 759 WP_274299340.1 class I SAM-dependent methyltransferase -
  JH277_RS05365 (JH277_05345) - 1291789..1292475 (+) 687 WP_258872788.1 glycosyltransferase family 9 protein -
  JH277_RS05370 (JH277_05350) - 1292478..1293320 (-) 843 WP_147308817.1 glycosyltransferase -
  JH277_RS05375 (JH277_05355) - 1293314..1294486 (-) 1173 WP_181920810.1 glycosyltransferase -
  JH277_RS05380 (JH277_05360) - 1294471..1295334 (-) 864 WP_116921534.1 glycosyltransferase -
  JH277_RS05385 (JH277_05365) - 1295384..1296235 (-) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  JH277_RS05390 (JH277_05370) pilB 1296391..1298124 (+) 1734 WP_274299341.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH277_RS05395 (JH277_05375) - 1298307..1299044 (-) 738 WP_274299342.1 zeta toxin family protein -
  JH277_RS05400 (JH277_05380) - 1299044..1299400 (-) 357 WP_139328384.1 hypothetical protein -
  JH277_RS05405 (JH277_05385) pilR 1299598..1300992 (-) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  JH277_RS05410 (JH277_05390) - 1301201..1302811 (-) 1611 WP_070690016.1 HAMP domain-containing sensor histidine kinase -
  JH277_RS05415 (JH277_05395) sucC 1303045..1304214 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  JH277_RS05420 (JH277_05400) sucD 1304239..1305114 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH277_RS05425 (JH277_05405) - 1305218..1305628 (+) 411 WP_323475224.1 DNA-binding protein -
  JH277_RS05430 (JH277_05410) - 1305632..1305934 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62846.04 Da        Isoelectric Point: 5.6058

>NTDB_id=523337 JH277_RS05390 WP_274299341.1 1296391..1298124(+) (pilB) [Xanthomonas campestris pv. raphani strain 576]
MNAVISANLVGITGIARRLVQDGALDETAARTAMEQAASARVPLPQWFAERKLVTASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=523337 JH277_RS05390 WP_274299341.1 1296391..1298124(+) (pilB) [Xanthomonas campestris pv. raphani strain 576]
ATGAACGCAGTGATCTCAGCCAATCTCGTTGGCATAACCGGCATAGCTCGTCGGCTGGTTCAAGACGGCGCACTTGATGA
AACGGCGGCCCGTACCGCCATGGAGCAAGCTGCTTCAGCCAGGGTTCCTCTGCCCCAGTGGTTTGCGGAGAGAAAGTTAG
TGACTGCATCCCAGCTTGCCGCCGCAAACGCGGTTGAGTTTGGCATGCCTCTGATGGACGTTTCCGTGTTCGATGCCAGC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTACCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTCCATACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGATCAGATCCGTCGTACCTTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTTGGCTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGACGACACGCCGGTGGTGAAATTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGATA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCTTGAAGGTAATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGTTGCGTATCCTGGACGCCAGTGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTAACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGACGAGACGCGCAATATTTCAACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GTGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGCCATATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTGACGCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.44

0.549
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

51.937

98.44

0.511
  pilB Vibrio cholerae strain A1552

48.785

99.827

0.487
  pilF Neisseria gonorrhoeae MS11

48.063

98.44

0.473
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.826

97.574

0.447
  pilB/pilB1 Synechocystis sp. PCC 6803

35.41

100

0.374