Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JH272_RS05830 Genome accession   NZ_CP066929
Coordinates   1374682..1375080 (+) Length   132 a.a.
NCBI ID   WP_104570927.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain CFBP12825     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1371562..1394152 1374682..1375080 within 0


Gene organization within MGE regions


Location: 1371562..1394152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH272_RS05815 (JH272_05790) coaE 1371562..1372185 (-) 624 WP_274316878.1 dephospho-CoA kinase -
  JH272_RS05820 (JH272_05795) - 1372199..1373062 (-) 864 WP_040940797.1 A24 family peptidase -
  JH272_RS05825 (JH272_05800) pilC 1373069..1374331 (-) 1263 WP_104570928.1 type II secretion system F family protein Machinery gene
  JH272_RS05830 (JH272_05805) pilA/pilAI 1374682..1375080 (+) 399 WP_104570927.1 pilin Machinery gene
  JH272_RS05835 (JH272_05810) - 1375181..1375603 (+) 423 WP_104570926.1 pilin -
  JH272_RS05840 (JH272_05815) - 1375662..1377482 (+) 1821 WP_274361049.1 hypothetical protein -
  JH272_RS05845 (JH272_05820) - 1377606..1378364 (+) 759 WP_274361050.1 class I SAM-dependent methyltransferase -
  JH272_RS05850 (JH272_05825) - 1378300..1379547 (+) 1248 WP_274361051.1 glycosyltransferase family 9 protein -
  JH272_RS05855 (JH272_05830) - 1379550..1380392 (-) 843 WP_147308817.1 glycosyltransferase -
  JH272_RS05860 (JH272_05835) - 1380386..1381558 (-) 1173 WP_228442823.1 glycosyltransferase -
  JH272_RS05865 (JH272_05840) - 1381543..1382406 (-) 864 WP_274361052.1 glycosyltransferase -
  JH272_RS05870 (JH272_05845) - 1382456..1383307 (-) 852 WP_108133901.1 glycosyltransferase family 2 protein -
  JH272_RS05875 (JH272_05850) pilB 1383463..1385199 (+) 1737 WP_040940802.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH272_RS05885 (JH272_05860) pilR 1387814..1389208 (-) 1395 WP_274361054.1 sigma-54 dependent transcriptional regulator Regulator
  JH272_RS05890 (JH272_05865) - 1389419..1391029 (-) 1611 WP_040940804.1 HAMP domain-containing sensor histidine kinase -
  JH272_RS05895 (JH272_05870) sucC 1391263..1392432 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  JH272_RS05900 (JH272_05875) sucD 1392457..1393332 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  JH272_RS05905 (JH272_05880) - 1393436..1393846 (+) 411 WP_029628876.1 DNA-binding protein -
  JH272_RS05910 (JH272_05885) - 1393850..1394152 (+) 303 WP_011038207.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 13656.88 Da        Isoelectric Point: 8.8372

>NTDB_id=523283 JH272_RS05830 WP_104570927.1 1374682..1375080(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain CFBP12825]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKSQATAGLAEITPGKTQYEVLVNEGTVPTTAAQIGLTTPTARCAV
VVSTSGITCTLNGNANKIKGKTISLNRSSADGTWSCVTGGTMDAKYKPAGCS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=523283 JH272_RS05830 WP_104570927.1 1374682..1375080(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain CFBP12825]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTAGTCGCGATCATCGCCATCCTGGCCGCCATTGCGCT
ACCGATGTATCAGGACTATGTTGCCAAGTCGCAGGCGACTGCCGGTCTGGCAGAAATCACTCCTGGCAAGACGCAGTATG
AAGTGTTGGTCAACGAAGGTACTGTTCCTACGACGGCCGCCCAGATTGGCTTGACAACGCCGACTGCGCGTTGCGCTGTT
GTCGTAAGCACCTCTGGCATTACATGCACCTTGAATGGCAACGCGAACAAGATTAAAGGCAAAACCATTAGCCTTAACCG
TAGTTCCGCAGATGGTACTTGGAGCTGTGTAACTGGCGGCACCATGGACGCGAAATACAAGCCCGCCGGCTGTTCCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

58.394

100

0.606
  pilA Acinetobacter baumannii strain A118

51.064

100

0.545
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.667

100

0.477
  pilA Pseudomonas aeruginosa PAK

41.06

100

0.47
  pilA2 Legionella pneumophila strain ERS1305867

43.066

100

0.447
  pilA2 Legionella pneumophila str. Paris

43.066

100

0.447
  comP Acinetobacter baylyi ADP1

39.456

100

0.439
  pilA Vibrio cholerae strain A1552

39.583

100

0.432
  pilA Vibrio cholerae C6706

39.583

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.583

100

0.432
  pilA/pilA1 Eikenella corrodens VA1

36.242

100

0.409
  pilE Neisseria gonorrhoeae MS11

34.416

100

0.402
  pilA Vibrio parahaemolyticus RIMD 2210633

41.6

94.697

0.394
  pilA Haemophilus influenzae Rd KW20

38.346

100

0.386