Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GVI69_RS17675 Genome accession   NZ_CP066101
Coordinates   3703017..3703997 (-) Length   326 a.a.
NCBI ID   WP_006811926.1    Uniprot ID   A0AAX3Z0L2
Organism   Enterobacter hormaechei strain Eho-8     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3698017..3708997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVI69_RS17645 (GVI69_17605) - 3698894..3699391 (+) 498 WP_006811920.1 SprT family zinc-dependent metalloprotease -
  GVI69_RS17650 (GVI69_17610) endA 3699486..3700193 (+) 708 WP_006811921.1 deoxyribonuclease I -
  GVI69_RS17655 (GVI69_17615) rsmE 3700245..3700976 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GVI69_RS17660 (GVI69_17620) gshB 3701008..3701955 (+) 948 WP_006811923.1 glutathione synthase -
  GVI69_RS17665 (GVI69_17625) - 3702030..3702590 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  GVI69_RS17670 (GVI69_17630) ruvX 3702590..3703006 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  GVI69_RS17675 (GVI69_17635) pilT 3703017..3703997 (-) 981 WP_006811926.1 type IV pilus twitching motility protein PilT Machinery gene
  GVI69_RS17680 (GVI69_17640) - 3704015..3704716 (+) 702 WP_006811927.1 YggS family pyridoxal phosphate-dependent enzyme -
  GVI69_RS17685 (GVI69_17645) - 3704738..3705304 (+) 567 WP_006811928.1 YggT family protein -
  GVI69_RS17690 (GVI69_17650) yggU 3705301..3705597 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  GVI69_RS17695 (GVI69_17655) - 3705601..3706194 (+) 594 WP_006811929.1 XTP/dITP diphosphatase -
  GVI69_RS17700 (GVI69_17660) hemW 3706187..3707335 (+) 1149 WP_006811930.1 radical SAM family heme chaperone HemW -
  GVI69_RS17705 (GVI69_17665) - 3707392..3707754 (+) 363 WP_006811931.1 endonuclease domain-containing protein -
  GVI69_RS17710 (GVI69_17670) - 3707928..3708644 (-) 717 WP_006811932.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35701.67 Da        Isoelectric Point: 6.5004

>NTDB_id=517436 GVI69_RS17675 WP_006811926.1 3703017..3703997(-) (pilT) [Enterobacter hormaechei strain Eho-8]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIVQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=517436 GVI69_RS17675 WP_006811926.1 3703017..3703997(-) (pilT) [Enterobacter hormaechei strain Eho-8]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCGCCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCAGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAACTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACTCTGGCCGCGATGGTGGATTTCCTCAATCACCACACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGGTGTCTGATCCAGCAGCGGGAAATTGGCCAGCACAGCCCCTCCTTTGCCGAGGCCCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGTGAGTTGCGCGACAGCGAAACAATACGCCTGGCACTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTCCACACGCGCGGTGCAGCGCAGGCGATTGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGCAATCAGCTGGCGGGAAGCCTACGTGCGGTACTGGCGCAAAAACTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTACCGGGCATTGTTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377