Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   I6H78_RS04495 Genome accession   NZ_CP066059
Coordinates   933276..934217 (-) Length   313 a.a.
NCBI ID   WP_198460194.1    Uniprot ID   -
Organism   Streptococcus oralis strain FDAARGOS_1021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928276..939217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H78_RS04455 (I6H78_04455) comYH 928875..929828 (-) 954 WP_198460191.1 class I SAM-dependent methyltransferase Machinery gene
  I6H78_RS04460 (I6H78_04460) - 929887..930477 (-) 591 WP_198460192.1 class I SAM-dependent methyltransferase -
  I6H78_RS04465 (I6H78_04465) comGG/cglG 930510..930923 (-) 414 WP_000265643.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6H78_RS04470 (I6H78_04470) comGF/cglF 930901..931362 (-) 462 WP_000250706.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6H78_RS04475 (I6H78_04475) comGE/cglE 931325..931627 (-) 303 WP_000413374.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6H78_RS04480 (I6H78_04480) comGD/cglD 931590..932024 (-) 435 WP_198460193.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6H78_RS04485 (I6H78_04485) comGC/cglC 931987..932310 (-) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  I6H78_RS04490 (I6H78_04490) comGB/cglB 932312..933328 (-) 1017 WP_198460227.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6H78_RS04495 (I6H78_04495) comGA/cglA/cilD 933276..934217 (-) 942 WP_198460194.1 competence type IV pilus ATPase ComGA Machinery gene
  I6H78_RS04500 (I6H78_04500) - 934280..934645 (-) 366 WP_000286396.1 DUF1033 family protein -
  I6H78_RS04505 (I6H78_04505) nagA 934798..935949 (-) 1152 WP_198460195.1 N-acetylglucosamine-6-phosphate deacetylase -
  I6H78_RS04510 (I6H78_04510) - 936090..937907 (-) 1818 WP_198460196.1 acyltransferase family protein -
  I6H78_RS04515 (I6H78_04515) tgt 938041..939183 (-) 1143 WP_198460197.1 tRNA guanosine(34) transglycosylase Tgt -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35417.35 Da        Isoelectric Point: 5.7856

>NTDB_id=516963 I6H78_RS04495 WP_198460194.1 933276..934217(-) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_1021]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQGLHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQEEMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNAQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=516963 I6H78_RS04495 WP_198460194.1 933276..934217(-) (comGA/cglA/cilD) [Streptococcus oralis strain FDAARGOS_1021]
ATGGTACAAGAAATTGCACAGAAAATTATTGCTACTGCGAAAGAAAAGAAGGCCCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTATGAACTTCACATGCGGGTTGGAGACGAACGGTGTCTAGTTGACTCCTATGAGTTTGATGTTTTAGCTGCTG
TGATTAGTCATTTTAAGTTTGTGGCGGGTATGAACGTAGGAGAGAAGAGGCGCAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTATCTTCTCTGCGTTTGTCTACCGTGGGAGATTATCGGGGGCATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGGCCTGCATTTCTGGTTTCAAGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGGCTCT
ATCTCTTTGCAGGTCCAGTCGGAAGTGGCAAGACGACTCTGATGCACGAATTAGCTAAGTCACTCTTTAAGGGACAACAA
GTTATGTCTATCGAAGACCCTGTCGAAATCAAGCAAGAAGAGATGTTGCAATTGCAGTTGAATGAGGCGATTGGCTTGAC
CTATGAAAATCTCATCAAACTGTCTCTCCGACATCGTCCGGATCTCCTGATTATCGGTGAAATTCGGGATAGTGAGACGG
CGCGTGCGGTTGTCAGAGCCAGTTTGACAGGGGCGACGGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAGGAACACCAGCCAACCAGCTGGAATGCGCAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.776

100

0.898
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae D39

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae R6

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

87.859

100

0.879
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.042

0.597
  comGA Lactococcus lactis subsp. cremoris KW2

54.487

99.681

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

44.444

83.387

0.371