Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   I6H54_RS30195 Genome accession   NZ_CP066006
Coordinates   6644825..6645268 (-) Length   147 a.a.
NCBI ID   WP_034389792.1    Uniprot ID   -
Organism   Delftia acidovorans strain FDAARGOS_997     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6631299..6644759 6644825..6645268 flank 66


Gene organization within MGE regions


Location: 6631299..6645268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H54_RS30145 (I6H54_30145) - 6631650..6632918 (+) 1269 WP_162269879.1 oligosaccharide flippase family protein -
  I6H54_RS30150 (I6H54_30150) - 6632902..6633642 (+) 741 WP_080736784.1 class I SAM-dependent methyltransferase -
  I6H54_RS30155 (I6H54_30155) - 6633749..6635041 (+) 1293 WP_155294166.1 hypothetical protein -
  I6H54_RS30160 (I6H54_30160) - 6635170..6636417 (+) 1248 WP_080736785.1 glycosyltransferase -
  I6H54_RS30165 (I6H54_30165) - 6636436..6638448 (+) 2013 WP_155294167.1 hypothetical protein -
  I6H54_RS30170 (I6H54_30170) glf 6638481..6639578 (+) 1098 WP_230590700.1 UDP-galactopyranose mutase -
  I6H54_RS30175 (I6H54_30175) - 6639586..6640734 (+) 1149 WP_198488078.1 glycosyltransferase family 4 protein -
  I6H54_RS30180 (I6H54_30180) - 6640739..6641956 (-) 1218 WP_034389790.1 glycosyltransferase -
  I6H54_RS30185 (I6H54_30185) - 6641953..6642804 (-) 852 WP_034389791.1 glycosyltransferase family 2 protein -
  I6H54_RS30190 (I6H54_30190) - 6642801..6644759 (-) 1959 WP_174549395.1 tetratricopeptide repeat protein -
  I6H54_RS30195 (I6H54_30195) pilA2 6644825..6645268 (-) 444 WP_034389792.1 pilin Machinery gene

Sequence


Protein


Download         Length: 147 a.a.        Molecular weight: 15318.61 Da        Isoelectric Point: 9.8522

>NTDB_id=516282 I6H54_RS30195 WP_034389792.1 6644825..6645268(-) (pilA2) [Delftia acidovorans strain FDAARGOS_997]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRSKVSEGLTLAASAKTAVSEHRLSQGTWPATSAAAGYTSPNTK
YVSGITIQDGLITITYKTGSSGSGVASGNDKLVLSALSVVGAVDWKCKRPAANPVDAKYLPANCRQS

Nucleotide


Download         Length: 444 bp        

>NTDB_id=516282 I6H54_RS30195 WP_034389792.1 6644825..6645268(-) (pilA2) [Delftia acidovorans strain FDAARGOS_997]
ATGAAGCGTTCCATCCAGCAAGGTTTTACCTTGATCGAATTGATGATCGTTGTGGCGATCATCGGCATTTTGGCAGCCGT
TGCGCTGCCGGCTTATCAGGATTACACGGTGCGTTCCAAAGTGTCGGAAGGCCTGACCCTGGCAGCCTCTGCCAAGACCG
CCGTCTCGGAGCACCGTCTGAGCCAAGGCACCTGGCCCGCCACCAGCGCTGCTGCTGGCTACACTTCCCCCAACACAAAG
TATGTGAGCGGCATCACCATTCAAGATGGCTTGATTACCATCACATACAAGACAGGCTCTTCCGGCTCTGGCGTTGCTAG
CGGCAATGACAAGCTGGTGCTCTCGGCTCTGTCGGTTGTCGGTGCCGTGGACTGGAAGTGCAAGCGCCCTGCCGCCAACC
CTGTGGATGCCAAGTACCTGCCGGCCAACTGCCGCCAGAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.521

96.599

0.517

  pilA2 Legionella pneumophila strain ERS1305867

53.521

96.599

0.517

  pilA Ralstonia pseudosolanacearum GMI1000

44.91

100

0.51

  pilE Neisseria gonorrhoeae MS11

43.75

100

0.476

  pilE Neisseria gonorrhoeae strain FA1090

43.478

100

0.476

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.17

100

0.463

  comP Acinetobacter baylyi ADP1

44.218

100

0.442

  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.624

100

0.442

  pilA/pilA1 Eikenella corrodens VA1

38.217

100

0.408

  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.255

100

0.388

  pilA Vibrio cholerae C6706

37.333

100

0.381

  pilA Vibrio cholerae strain A1552

37.333

100

0.381

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.333

100

0.381

  pilA Vibrio campbellii strain DS40M4

36.424

100

0.374

  pilA Acinetobacter baumannii strain A118

36.486

100

0.367