Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   YSA_RS11395 Genome accession   NC_017986
Coordinates   2343075..2344073 (-) Length   332 a.a.
NCBI ID   WP_014754737.1    Uniprot ID   I3UUD4
Organism   Pseudomonas putida ND6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338075..2349073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YSA_RS11370 (YSA_04314) argS 2339207..2340943 (+) 1737 WP_014754734.1 arginine--tRNA ligase -
  YSA_RS11375 (YSA_04317) - 2340945..2341643 (+) 699 WP_003249122.1 SPOR domain-containing protein -
  YSA_RS11380 (YSA_04319) - 2341735..2342154 (-) 420 WP_003249120.1 TM2 domain-containing protein -
  YSA_RS11385 (YSA_04322) - 2342321..2342947 (+) 627 WP_003249118.1 C40 family peptidase -
  YSA_RS11395 (YSA_04323) pilT 2343075..2344073 (-) 999 WP_014754737.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  YSA_RS11400 (YSA_04325) - 2344139..2344825 (+) 687 WP_004575484.1 YggS family pyridoxal phosphate-dependent enzyme -
  YSA_RS11405 (YSA_04327) proC 2344864..2345682 (+) 819 WP_004575483.1 pyrroline-5-carboxylate reductase -
  YSA_RS11410 (YSA_04328) - 2345693..2346283 (+) 591 WP_012053960.1 YggT family protein -
  YSA_RS11415 (YSA_04329) - 2346383..2347522 (+) 1140 WP_003249108.1 homoserine O-acetyltransferase -
  YSA_RS11420 (YSA_04333) metW 2347530..2348150 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  YSA_RS11425 (YSA_04334) - 2348173..2348607 (+) 435 WP_014754738.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35981.69 Da        Isoelectric Point: 7.5270

>NTDB_id=51577 YSA_RS11395 WP_014754737.1 2343075..2344073(-) (pilT) [Pseudomonas putida ND6]
MDVTDLLARAVDAGASDLHLATGQIPMLRLDGELQRMGLPPLSQGALIEGMASVLDDDQRRQWAQGDEMDLALELPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVSELDLADVFQAVAHCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEVIHASQRSLVNQREMGRHSRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSATSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLIREGRLAQLSSVMQGGAAEGMLTMEGAL
RRLRERGLIKGL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=51577 YSA_RS11395 WP_014754737.1 2343075..2344073(-) (pilT) [Pseudomonas putida ND6]
ATGGATGTGACCGACCTGTTGGCCCGAGCTGTGGATGCGGGGGCTTCAGACCTGCACCTGGCGACGGGCCAGATTCCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGTATGGGCTTGCCGCCCTTGAGCCAGGGTGCCTTGATCGAGGGCATGGCGAGCG
TGCTGGATGACGATCAGCGCCGGCAGTGGGCCCAAGGCGATGAAATGGACCTTGCGCTGGAGTTGCCGGCGCTTGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGAATGGCCCGGCGGTTACCTTTCGGTTGATTCCGGGGCGCATTGCGACGGTGAG
TGAGCTGGACCTTGCGGATGTGTTTCAAGCTGTTGCGCATTGCCGGGATGGCTTGATCCTCGTGGGCGGGCCGACCGGCA
GTGGCAAGTCCAGTACCCTGGCAACGCTGCTCGACCAGCTGAACCGCGAGCGGGCGCTGCACATCATTACCCTGGAAGAC
CCTGTCGAGGTCATCCATGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATGGGGCGCCATAGTCGCGGCTTTGCTCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGGAAACCATTCGCCTGG
CCCTGCGCGCGGCCGAAACCGGGCACCTGGTGCTGGCAACCGTGCATGCGCGTTCGGCGACCAGCAGCATCGACAGGTTG
GTGGAGGTTTTTGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGCGTGGGGGGTGGGCGGGTGGCTGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGTAACCTGATTC
GGGAGGGGCGCTTGGCGCAGTTGTCTTCGGTGATGCAGGGCGGGGCGGCGGAGGGGATGCTGACGATGGAGGGGGCGTTG
CGGAGGCTGAGGGAGCGAGGGTTGATCAAAGGCTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3UUD4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.368

99.096

0.509
  pilT Acinetobacter baumannii D1279779

50.76

99.096

0.503
  pilT Acinetobacter baumannii strain A118

50.76

99.096

0.503
  pilT Acinetobacter nosocomialis M2

50.76

99.096

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.456

99.096

0.5
  pilT Acinetobacter baylyi ADP1

50.303

99.398

0.5
  pilT Neisseria meningitidis 8013

49.697

99.398

0.494
  pilT Neisseria gonorrhoeae MS11

49.394

99.398

0.491
  pilT Vibrio cholerae strain A1552

49.544

99.096

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

99.096

0.491
  pilT Legionella pneumophila strain ERS1305867

49.24

99.096

0.488
  pilT Legionella pneumophila strain Lp02

49.24

99.096

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.726

100

0.473
  pilU Pseudomonas stutzeri DSM 10701

38.182

99.398

0.38
  pilU Vibrio cholerae strain A1552

37.346

97.59

0.364

Multiple sequence alignment