NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	I6G67_RS01210	Genome accession	NZ_CP065666
Coordinates	235557..235988 (-)	Length	143 a.a.
NCBI ID	WP_004691476.1	Uniprot ID	A0A380U735
Organism	Acinetobacter johnsonii strain FDAARGOS_910
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 230557..240988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6G67_RS01195 (I6G67_01195)	-	232297..234354 (+)	2058	WP_004691479.1	hypothetical protein	-
I6G67_RS01200 (I6G67_01200)	-	234358..234717 (+)	360	WP_004691478.1	hypothetical protein	-
I6G67_RS01205 (I6G67_01205)	-	234902..235177 (-)	276	WP_004691477.1	HNH endonuclease family protein	-
I6G67_RS17800	-	235261..235560 (+)	300	Protein_219	hypothetical protein	-
I6G67_RS01210 (I6G67_01210)	pilA2	235557..235988 (-)	432	WP_004691476.1	pilin	Machinery gene
I6G67_RS01215 (I6G67_01215)	-	236357..236527 (+)	171	WP_004691475.1	hypothetical protein	-
I6G67_RS01220 (I6G67_01220)	-	236753..237193 (+)	441	WP_004691474.1	pilin	-
I6G67_RS01225 (I6G67_01225)	tfpZ	237489..238235 (+)	747	WP_004691473.1	TfpX/TfpZ family type IV pilin accessory protein	-
I6G67_RS01230 (I6G67_01230)	bfr	238294..238758 (-)	465	WP_004691472.1	bacterioferritin	-
I6G67_RS01235 (I6G67_01235)	-	239013..239207 (-)	195	WP_010327639.1	bacterioferritin-associated ferredoxin	-
I6G67_RS01240 (I6G67_01240)	-	239361..239744 (-)	384	WP_004691470.1	RidA family protein	-

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14590.94 Da Isoelectric Point: 8.1079

                                >NTDB_id=512774 I6G67_RS01210 WP_004691476.1 235557..235988(-) (pilA2) [Acinetobacter johnsonii strain FDAARGOS_910]

MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVSEALTTIAPLKSTVSENIITNNLVTSPLTATACSGISTVTA

TDNVASGTCALGVVSVTTTAKAGAVTIKLTPTATVTDGIVSNVKWVCTADKEKYAPASCRTGA

Nucleotide

Download Length: 432 bp

                                >NTDB_id=512774 I6G67_RS01210 WP_004691476.1 235557..235988(-) (pilA2) [Acinetobacter johnsonii strain FDAARGOS_910]

ATGAACGCTCAAAAAGGCTTTACGTTGATCGAGCTGATGATTGTTGTCGCTATTATCGGTATTTTGGCTGCAATTGCGAT

TCCTGCTTATCAGGATTACACAATTCGTGCTCGTGTTTCAGAAGCTCTAACTACTATCGCTCCTTTGAAAAGTACAGTTA

GCGAAAATATTATTACAAATAATCTTGTAACAAGCCCTCTTACTGCTACAGCATGTTCTGGAATATCTACAGTCACTGCT

ACCGATAACGTAGCTTCTGGAACTTGTGCGTTAGGTGTTGTTTCAGTAACTACTACTGCTAAAGCAGGTGCAGTAACTAT

TAAATTAACTCCTACTGCAACAGTTACTGATGGCATCGTATCTAATGTTAAATGGGTTTGTACAGCCGATAAGGAGAAAT

ATGCACCTGCTTCTTGTCGTACTGGAGCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-140)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A380U735

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	57.857	97.902	0.566
comP	Acinetobacter baylyi ADP1	53.289	100	0.566
pilA2	Legionella pneumophila strain ERS1305867	57.143	97.902	0.559
pilA	Ralstonia pseudosolanacearum GMI1000	46.951	100	0.538
pilA	Pseudomonas aeruginosa PAK	43.506	100	0.469
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.755	100	0.448
pilE	Neisseria gonorrhoeae MS11	37.126	100	0.434
pilE	Neisseria gonorrhoeae strain FA1090	38.608	100	0.427
pilA	Acinetobacter baumannii strain A118	40	100	0.406
pilA	Vibrio cholerae strain A1552	37.086	100	0.392
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	37.086	100	0.392
pilA	Vibrio cholerae C6706	37.086	100	0.392
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.286	97.902	0.385
pilA	Haemophilus influenzae 86-028NP	42.857	88.112	0.378
pilA	Haemophilus influenzae Rd KW20	41.732	88.811	0.371

Detailed information NTG0512774