Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IG609_RS03640 Genome accession   NZ_CP065177
Coordinates   791148..792188 (+) Length   346 a.a.
NCBI ID   WP_193397337.1    Uniprot ID   A0A9Q2ERB5
Organism   Pectobacterium quasiaquaticum strain A477-S1-J17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786148..797188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG609_RS03605 (IG609_003595) - 786659..786781 (+) 123 Protein_691 GNAT family N-acetyltransferase -
  IG609_RS03610 (IG609_003600) hemW 786857..787996 (-) 1140 WP_193397340.1 radical SAM family heme chaperone HemW -
  IG609_RS03615 (IG609_003605) - 787989..788582 (-) 594 WP_193397339.1 XTP/dITP diphosphatase -
  IG609_RS03620 (IG609_003610) yggU 788600..788890 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  IG609_RS03625 (IG609_003615) - 788887..789441 (-) 555 WP_010295003.1 YggT family protein -
  IG609_RS03630 (IG609_003620) proC 789491..790312 (-) 822 WP_193397469.1 pyrroline-5-carboxylate reductase -
  IG609_RS03635 (IG609_003625) - 790414..791127 (-) 714 WP_193397338.1 YggS family pyridoxal phosphate-dependent enzyme -
  IG609_RS03640 (IG609_003630) pilT 791148..792188 (+) 1041 WP_193397337.1 type IV pilus twitching motility protein PilT Machinery gene
  IG609_RS03645 (IG609_003635) - 792190..793761 (-) 1572 WP_193397336.1 methyl-accepting chemotaxis protein -
  IG609_RS03650 (IG609_003640) nsrR 794089..794514 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  IG609_RS03655 (IG609_003645) rnr 794556..797012 (+) 2457 WP_193397335.1 ribonuclease R -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37615.87 Da        Isoelectric Point: 7.0925

>NTDB_id=507687 IG609_RS03640 WP_193397337.1 791148..792188(+) (pilT) [Pectobacterium quasiaquaticum strain A477-S1-J17]
MELDEWMARSVKHNASDLHLCSGHPPMLRVDGRLQPENTLPRLTSDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLEALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSRDRHVITLE
DPIEFIHASRRCLIQQREIGTHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTASAAASNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHRFSAGPTTGGAIHP

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=507687 IG609_RS03640 WP_193397337.1 791148..792188(+) (pilT) [Pectobacterium quasiaquaticum strain A477-S1-J17]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGAT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACCTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTTCGGCAGGCTGGTCAGGTGGACGGCGCGTTAATGCTGCCTGATGGGCAA
CGCTTGCGGGTGAACGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTGTTATCCCGTCCGTTCAGCCTTCATT
GGAAGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAGCCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCTACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCCGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTACGCACAGCGCATCGTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCTGATGTCATTCTGCTAGGAGAATTGCGCGATACAGAGACGATTCGGC
TGGCTCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGCTGCCCGCTGCGCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGAGTGCGGCAGCCAGCAACTTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAGACAGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACCGTTTTAGCGCTGGGCCGACCACAGGCGGTGCAATCCATCCGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

95.376

0.497
  pilT Vibrio cholerae strain A1552

52.121

95.376

0.497
  pilT Neisseria meningitidis 8013

50.904

95.954

0.488
  pilT Neisseria gonorrhoeae MS11

50.602

95.954

0.486
  pilT Acinetobacter baylyi ADP1

50.151

95.665

0.48
  pilT Acinetobacter baumannii strain A118

49.849

95.665

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.849

95.665

0.477
  pilT Legionella pneumophila strain Lp02

49.849

95.665

0.477
  pilT Legionella pneumophila strain ERS1305867

49.849

95.665

0.477
  pilT Acinetobacter nosocomialis M2

49.849

95.665

0.477
  pilT Acinetobacter baumannii D1279779

49.849

95.665

0.477
  pilT Pseudomonas aeruginosa PAK

49.547

95.665

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.042

96.243

0.405
  pilU Vibrio cholerae strain A1552

39.759

95.954

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.339

96.243

0.379