Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I1H34_RS15070 Genome accession   NZ_CP064923
Coordinates   3240891..3242012 (-) Length   373 a.a.
NCBI ID   WP_212661865.1    Uniprot ID   -
Organism   Acaryochloris marina S15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3235891..3247012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I1H34_RS15045 (I1H34_15035) cdaA 3236760..3237674 (+) 915 WP_212661860.1 diadenylate cyclase CdaA -
  I1H34_RS15050 (I1H34_15040) - 3237671..3238423 (+) 753 WP_212661861.1 isoprenyl transferase -
  I1H34_RS15055 (I1H34_15045) - 3238486..3238767 (-) 282 WP_212661862.1 DUF3143 domain-containing protein -
  I1H34_RS15060 (I1H34_15050) - 3238958..3239416 (-) 459 WP_212661863.1 J domain-containing protein -
  I1H34_RS15065 (I1H34_15055) - 3239601..3240821 (-) 1221 WP_212661864.1 type II secretion system F family protein -
  I1H34_RS15070 (I1H34_15060) pilT 3240891..3242012 (-) 1122 WP_212661865.1 type IV pilus twitching motility protein PilT Machinery gene
  I1H34_RS33110 - 3242163..3243500 (-) 1338 Protein_2817 ISKra4 family transposase -
  I1H34_RS15090 (I1H34_15080) pilB/pilB1 3243494..3245557 (-) 2064 WP_249369266.1 GspE/PulE family protein Machinery gene
  I1H34_RS15095 (I1H34_15085) grpE 3246177..3246965 (+) 789 WP_212661869.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41117.04 Da        Isoelectric Point: 6.6559

>NTDB_id=505659 I1H34_RS15070 WP_212661865.1 3240891..3242012(-) (pilT) [Acaryochloris marina S15]
MDYMIEDLMEEVVERGGSDLHLSVGLPPYIRINGKLTPTDYDPLSQEQCQRLIFSMLNNTQRKQLEQTWELDCSYGVRGL
ARFRVNVYKDRGTYAAALRALSSKIPAIETLGLPNIVREMSEKPRGLVLVTGPTGSGKTTTLASMINNINLTRAEHILTV
EDPIEFVYEPIKSLIHQRQVGEDTKSFSNALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQTQVRVQLSNSLVAVFSQTLVTRKNPKPGEFGRVMAQEIMVSTPGIANLIREGKTSQIYSAIQTGGKLGM
VTLEKVLAELYQAGSISFESAVSKTSRPDEMQRLMGAAPMGNQRGAGATSYRR

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=505659 I1H34_RS15070 WP_212661865.1 3240891..3242012(-) (pilT) [Acaryochloris marina S15]
ATGGACTACATGATTGAAGACCTAATGGAAGAAGTCGTTGAGCGAGGAGGCTCTGATTTACACCTCTCAGTAGGCTTACC
TCCCTACATTCGTATCAACGGAAAATTAACTCCTACTGATTATGATCCACTGAGTCAAGAGCAATGTCAGCGTCTTATCT
TTAGCATGCTGAATAATACTCAACGGAAACAATTAGAGCAAACATGGGAATTAGATTGCTCTTACGGTGTCAGAGGCCTA
GCTCGTTTTAGGGTTAATGTCTATAAGGATCGAGGTACTTATGCTGCTGCATTACGAGCACTCAGCTCTAAGATTCCTGC
TATAGAAACGTTGGGATTACCAAATATTGTCCGAGAAATGTCTGAGAAGCCCCGTGGTTTGGTACTAGTGACTGGTCCCA
CAGGCTCGGGAAAAACAACGACCCTTGCCTCCATGATTAACAATATCAATCTAACTCGGGCTGAACATATTCTCACGGTC
GAAGATCCCATTGAATTTGTTTATGAACCTATTAAAAGCCTGATCCACCAGCGTCAAGTTGGTGAAGATACCAAGAGCTT
TTCGAATGCACTGCGGGCTGCTCTCCGTGAAGATCCAGATGTTATTCTTGTAGGGGAAATGCGGGATCTTGAAACGATTC
AACTTGCTATTTCTGCAGCAGAAACCGGTCACTTAGTTTTTGGAACACTTCATACTAGTTCAGCAGCCCAAACTGTTGAT
CGGATGGTAGATGTATTTCCGCCTGAGCAACAGACTCAAGTTCGAGTGCAATTGTCTAACTCTTTGGTGGCTGTATTTAG
CCAAACGCTAGTGACGCGGAAAAATCCGAAACCAGGTGAGTTTGGGAGAGTCATGGCACAAGAAATTATGGTATCCACTC
CCGGAATTGCTAACTTAATTCGTGAAGGAAAAACATCTCAGATATACTCTGCTATTCAAACTGGAGGAAAGTTAGGAATG
GTTACTTTAGAGAAAGTCCTAGCTGAACTCTATCAGGCTGGCTCGATTTCCTTTGAATCAGCTGTATCCAAGACATCCCG
TCCTGATGAAATGCAGCGATTAATGGGAGCAGCCCCTATGGGTAATCAACGTGGCGCTGGTGCCACTTCTTATCGGCGAT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.493

92.493

0.504
  pilT Pseudomonas stutzeri DSM 10701

53.602

93.029

0.499
  pilT Acinetobacter baylyi ADP1

52.754

92.493

0.488
  pilT Acinetobacter nosocomialis M2

52.754

92.493

0.488
  pilT Acinetobacter baumannii D1279779

52.754

92.493

0.488
  pilT Acinetobacter baumannii strain A118

52.754

92.493

0.488
  pilT Legionella pneumophila strain Lp02

52.464

92.493

0.485
  pilT Legionella pneumophila strain ERS1305867

52.464

92.493

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.58

95.71

0.475
  pilT Neisseria meningitidis 8013

49.856

93.029

0.464
  pilT Neisseria gonorrhoeae MS11

49.568

93.029

0.461
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

91.153

0.458
  pilT Vibrio cholerae strain A1552

50.294

91.153

0.458
  pilU Pseudomonas stutzeri DSM 10701

39.031

94.102

0.367