Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I1H34_RS15090 Genome accession   NZ_CP064923
Coordinates   3243494..3245557 (-) Length   687 a.a.
NCBI ID   WP_249369266.1    Uniprot ID   -
Organism   Acaryochloris marina S15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3238494..3250557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I1H34_RS15060 (I1H34_15050) - 3238958..3239416 (-) 459 WP_212661863.1 J domain-containing protein -
  I1H34_RS15065 (I1H34_15055) - 3239601..3240821 (-) 1221 WP_212661864.1 type II secretion system F family protein -
  I1H34_RS15070 (I1H34_15060) pilT 3240891..3242012 (-) 1122 WP_212661865.1 type IV pilus twitching motility protein PilT Machinery gene
  I1H34_RS33110 - 3242163..3243500 (-) 1338 Protein_2817 ISKra4 family transposase -
  I1H34_RS15090 (I1H34_15080) pilB/pilB1 3243494..3245557 (-) 2064 WP_249369266.1 GspE/PulE family protein Machinery gene
  I1H34_RS15095 (I1H34_15085) grpE 3246177..3246965 (+) 789 WP_212661869.1 nucleotide exchange factor GrpE -
  I1H34_RS15100 (I1H34_15090) dnaK 3247129..3249117 (+) 1989 WP_212661870.1 molecular chaperone DnaK -
  I1H34_RS15105 (I1H34_15095) dnaJ 3249167..3250291 (+) 1125 WP_212661871.1 molecular chaperone DnaJ -
  I1H34_RS15110 (I1H34_15100) - 3250288..3250548 (+) 261 WP_212661872.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 687 a.a.        Molecular weight: 75865.70 Da        Isoelectric Point: 4.8983

>NTDB_id=505660 I1H34_RS15090 WP_249369266.1 3243494..3245557(-) (pilB/pilB1) [Acaryochloris marina S15]
MVNSSSQRRALVTRHNFSPFGNKLISTGYVDQDQMREATLESRRSGRSLVEILQGLTGQSLPPELLRQYKRQQLFELKIL
YGIDSLDLELSQIPTDQIEELIQNYISLDLCRRYQLVPLTLDQETDPATLLVAMVDPDNLGALDDLGRILREKNLTLQRM
VIAVEDYQQLISKYLDQQAAQQKANEKPIEFDLDDDLDAIAGLDDLPTDELETDLGAALQDAETAPIIGLVNKILAKALQ
SGVSDIHIEPQEDHLRVRFRKDGVLQQAFDPLPRKIIPAVISRFKIISELDIAERRLPQDGRIRRLFQGRKIDFRVNTLP
SRYGEKIVLRILDNSSTQLGLDKLVADEDSLNIVREMVSRPFGLILVTGPTGSGKTTTLYSALAECNHPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVARLAEMEV
EPFMVSASLLGVVAQRLVRRVCSDCKVPYQPSPSELGRFGLSASPGTEITFYKANSLSADELQTAQATGQTVCSKCNGIG
YKGRCGVYEVMQITETLQTLITEGAPTERIKEVAVEEGMKTLLAYSLELVKKGDTTLAEVERVTFTDSGLEAELKAKRKT
SLTGVSKGSGMHGSKKNGSRTIDPVPEPKTIMTLPSQLLTVLAHDEF

Nucleotide


Download         Length: 2064 bp        

>NTDB_id=505660 I1H34_RS15090 WP_249369266.1 3243494..3245557(-) (pilB/pilB1) [Acaryochloris marina S15]
ATGGTTAACTCTTCCTCACAGCGGCGTGCTCTAGTTACTCGGCACAATTTTTCCCCTTTTGGCAATAAGCTGATTTCAAC
AGGTTATGTTGACCAAGACCAAATGCGGGAAGCGACCTTAGAAAGTCGACGCTCCGGACGTTCTCTTGTCGAAATTCTTC
AGGGACTTACTGGACAGTCTTTACCACCAGAACTCCTACGCCAATATAAACGACAACAACTTTTTGAACTAAAGATCCTT
TATGGCATCGACTCCTTAGATCTTGAGTTAAGCCAAATTCCGACGGATCAAATCGAAGAATTGATCCAGAATTATATTTC
TCTTGACTTATGTCGACGCTATCAGTTGGTCCCCCTAACCCTTGATCAAGAAACGGACCCGGCAACGTTGCTGGTTGCAA
TGGTTGATCCAGATAATTTAGGTGCTCTGGACGATCTAGGACGAATTCTAAGGGAGAAAAATCTGACCTTGCAACGCATG
GTGATTGCGGTTGAAGACTATCAGCAACTCATCAGTAAGTATCTCGATCAGCAAGCTGCTCAGCAAAAAGCTAATGAAAA
ACCGATTGAGTTTGATTTAGACGATGACTTAGATGCGATCGCAGGTCTAGACGATCTCCCTACTGATGAACTTGAGACTG
ACCTCGGGGCAGCCCTGCAAGATGCGGAAACGGCTCCCATTATTGGTTTAGTTAACAAAATTCTGGCTAAAGCCTTGCAG
AGCGGTGTATCTGATATTCATATTGAACCGCAAGAAGATCACCTTCGTGTTCGATTCCGTAAAGATGGTGTGCTCCAACA
GGCCTTCGATCCGCTGCCCAGGAAGATTATTCCTGCTGTTATATCCCGTTTCAAAATCATTTCAGAGTTAGATATTGCTG
AGCGTCGCCTTCCTCAAGATGGTCGCATCCGGCGACTTTTTCAGGGACGCAAGATTGACTTTCGGGTTAATACCTTACCG
AGCCGATATGGAGAGAAGATTGTACTTCGTATTCTGGATAACTCCTCGACTCAACTGGGATTAGACAAACTTGTTGCAGA
CGAAGATAGTTTAAACATCGTTAGAGAGATGGTCAGTAGACCTTTTGGCCTGATTTTGGTAACAGGACCTACTGGTTCTG
GTAAAACCACCACTCTCTATTCTGCCTTAGCTGAATGTAATCACCCAGGTGTCAACATCAGTACCGCGGAGGACCCAATT
GAATATACCCTCCCAGGCCTGACCCAAGTCCAGGTGATTCGAGAAAAAGGCATGGACTTTGCCTCAATTTTGAGAGCTTT
TCTAAGGCAAGATCCAGATGTCATTCTAGTAGGTGAGACCCGAGATAAAGAAACCGCTAAAACAGCCATTGAAGCAGCGC
TAACAGGACACCTTGTGTTAACGACTCTGCATACGAATGATGCTGCTAGTGCTGTTGCTCGCCTTGCAGAAATGGAAGTC
GAGCCATTCATGGTTTCGGCGTCCTTATTGGGGGTTGTTGCCCAGCGTTTGGTACGGCGAGTATGTTCAGACTGTAAAGT
TCCCTATCAACCTTCACCCTCTGAGCTAGGTCGATTTGGGCTATCTGCTTCTCCAGGAACAGAGATCACCTTCTACAAAG
CTAATTCGTTATCAGCTGATGAACTTCAAACAGCTCAAGCTACAGGACAAACAGTTTGTTCAAAATGTAACGGTATTGGG
TATAAAGGGCGTTGTGGTGTTTACGAAGTTATGCAAATCACTGAAACCCTGCAAACATTGATTACTGAAGGGGCACCTAC
TGAGCGGATTAAAGAGGTGGCAGTTGAAGAAGGGATGAAAACCCTTCTAGCTTATAGCCTAGAACTAGTTAAGAAAGGGG
ATACTACCCTCGCTGAAGTTGAACGAGTTACCTTTACCGATAGTGGTCTAGAGGCTGAACTTAAAGCTAAGCGTAAAACA
TCTCTAACGGGCGTAAGCAAAGGATCGGGGATGCATGGCTCAAAGAAAAACGGGTCAAGGACAATAGATCCTGTACCCGA
ACCAAAAACTATCATGACCCTACCATCGCAATTGCTAACCGTGCTAGCCCATGACGAATTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(233-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.219

93.159

0.598