Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INS04_RS03345 Genome accession   NZ_CP063441
Coordinates   704018..704998 (+) Length   326 a.a.
NCBI ID   WP_193805665.1    Uniprot ID   -
Organism   Enterobacter asburiae strain SD4L     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699018..709998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INS04_RS03305 (INS04_03305) - 699102..699428 (+) 327 WP_014885198.1 YggL family protein -
  INS04_RS03310 (INS04_03310) - 699485..700201 (+) 717 WP_023309135.1 DUF2884 domain-containing protein -
  INS04_RS03315 (INS04_03315) - 700284..700622 (-) 339 WP_193805661.1 endonuclease domain-containing protein -
  INS04_RS03320 (INS04_03320) hemW 700686..701828 (-) 1143 WP_193805662.1 radical SAM family heme chaperone HemW -
  INS04_RS03325 (INS04_03325) - 701821..702414 (-) 594 WP_193805663.1 XTP/dITP diphosphatase -
  INS04_RS03330 (INS04_03330) yggU 702418..702714 (-) 297 WP_023333349.1 DUF167 family protein YggU -
  INS04_RS03335 (INS04_03335) - 702711..703277 (-) 567 WP_023309130.1 YggT family protein -
  INS04_RS03340 (INS04_03340) - 703299..704000 (-) 702 WP_193805664.1 YggS family pyridoxal phosphate-dependent enzyme -
  INS04_RS03345 (INS04_03345) pilT 704018..704998 (+) 981 WP_193805665.1 type IV pilus twitching motility protein PilT Machinery gene
  INS04_RS03350 (INS04_03350) ruvX 705009..705425 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  INS04_RS03355 (INS04_03355) - 705425..705985 (-) 561 WP_028014368.1 YqgE/AlgH family protein -
  INS04_RS03360 (INS04_03360) gshB 706073..707020 (-) 948 WP_045329321.1 glutathione synthase -
  INS04_RS03365 (INS04_03365) rsmE 707040..707771 (-) 732 WP_193805666.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INS04_RS03370 (INS04_03370) endA 707823..708530 (-) 708 WP_063439816.1 deoxyribonuclease I -
  INS04_RS03375 (INS04_03375) - 708625..709122 (-) 498 WP_045888327.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35583.60 Da        Isoelectric Point: 6.9108

>NTDB_id=495647 INS04_RS03345 WP_193805665.1 704018..704998(+) (pilT) [Enterobacter asburiae strain SD4L]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGFSLTLRLLPRSCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAAMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=495647 INS04_RS03345 WP_193805665.1 704018..704998(+) (pilT) [Enterobacter asburiae strain SD4L]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGTCGGCTTGAACCCGCTCCTTTTCCGCCTCCCGATGTCGGAGCGTTATTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGACAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTTTCTCTTTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCACAGCTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAATCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGTGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGTTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCAGCAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386