Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IDM36_RS18315 Genome accession   NZ_CP061801
Coordinates   3844197..3845180 (-) Length   327 a.a.
NCBI ID   WP_196390457.1    Uniprot ID   A0A7T0H0A4
Organism   Enterobacter mori strain HSW1412     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3839197..3850180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IDM36_RS18285 (IDM36_18290) - 3840019..3840516 (+) 498 WP_182272582.1 SprT family zinc-dependent metalloprotease -
  IDM36_RS18290 (IDM36_18295) endA 3840611..3841318 (+) 708 WP_196390456.1 deoxyribonuclease I -
  IDM36_RS18295 (IDM36_18300) rsmE 3841371..3842102 (+) 732 WP_182272584.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IDM36_RS18300 (IDM36_18305) gshB 3842134..3843081 (+) 948 WP_182272585.1 glutathione synthase -
  IDM36_RS18305 (IDM36_18310) - 3843165..3843725 (+) 561 WP_182272586.1 YqgE/AlgH family protein -
  IDM36_RS18310 (IDM36_18315) ruvX 3843725..3844141 (+) 417 WP_182272587.1 Holliday junction resolvase RuvX -
  IDM36_RS18315 (IDM36_18320) pilT 3844197..3845180 (-) 984 WP_196390457.1 type IV pilus twitching motility protein PilT Machinery gene
  IDM36_RS18320 (IDM36_18325) - 3845198..3845899 (+) 702 WP_182272588.1 YggS family pyridoxal phosphate-dependent enzyme -
  IDM36_RS18325 (IDM36_18330) - 3845921..3846487 (+) 567 WP_182272589.1 YggT family protein -
  IDM36_RS18330 (IDM36_18335) yggU 3846484..3846780 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  IDM36_RS18335 (IDM36_18340) - 3846785..3847378 (+) 594 WP_182272590.1 XTP/dITP diphosphatase -
  IDM36_RS18340 (IDM36_18345) hemW 3847371..3848513 (+) 1143 WP_182918923.1 radical SAM family heme chaperone HemW -
  IDM36_RS18345 (IDM36_18350) - 3848577..3848915 (+) 339 WP_182272592.1 endonuclease domain-containing protein -
  IDM36_RS18350 (IDM36_18355) - 3848982..3849701 (-) 720 WP_182918924.1 DUF2884 domain-containing protein -
  IDM36_RS18355 (IDM36_18360) - 3849755..3850081 (-) 327 WP_182272594.1 YggL family protein -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35794.90 Da        Isoelectric Point: 6.5002

>NTDB_id=484762 IDM36_RS18315 WP_196390457.1 3844197..3845180(-) (pilT) [Enterobacter mori strain HSW1412]
MVLEEIVALSVKHNVSDLHLCSDSPPRWRRLGYLEPAPFSSPDVEALLKTWLSDELQGVWWANGQVDFAVTLAGNQRLRG
SAFTHMKGISLTLRLLPLACPQLASLGAPRAIPELLSNDNGLILVTGATGSGKSTTLSAMVDFLNHHTDGHILTLEDPVE
LIYQSERCLIHQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAREKEPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRLL

Nucleotide


Download         Length: 984 bp        

>NTDB_id=484762 IDM36_RS18315 WP_196390457.1 3844197..3845180(-) (pilT) [Enterobacter mori strain HSW1412]
ATGGTATTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTTAGGTTACCTTGAACCTGCACCATTTTCTTCTCCTGACGTTGAAGCCTTATTAAAAACCTGGCTTAGCG
ATGAGCTGCAGGGGGTGTGGTGGGCGAACGGACAGGTGGATTTTGCCGTCACGCTTGCGGGAAATCAGCGTCTGCGCGGC
AGCGCATTTACCCACATGAAAGGTATCTCGCTAACGCTGCGGTTGCTGCCGCTTGCCTGTCCACAACTTGCCTCACTGGG
CGCGCCGAGAGCGATCCCGGAACTGTTGTCCAATGACAATGGGTTGATTCTGGTGACGGGCGCGACCGGAAGCGGAAAAT
CTACCACCCTCTCAGCGATGGTGGATTTTCTCAATCATCATACTGACGGGCACATTCTGACACTCGAAGATCCGGTGGAG
CTTATCTACCAAAGCGAGCGCTGTCTGATCCATCAACGGGAGATAGGTCAACACAGTCCGTCATTTGCCGAAGCACTACG
CAGTGCATTACGTGAAGATCCGGATGTGATCCTGTTGGGTGAATTGCGTGACAGCGAAACGATCCGTCTGGCGCTGACGG
CGGCAGAGACCGGACATCTGGTGCTGGCAACGCTGCATACGCGTGGTGCATCGCAGGCCATCGAGCGGCTGGTGGATACC
TTCCCGGCCCGGGAGAAAGAGCCAGTACGCAATCAGCTGGCAGGCAGCCTGCGGGCTGTACTGGCGCAAAAACTCCGTCA
GGATGTGCAGGGCGGGCGTGTGGCACTTTATGAGTTGCTGGTGAACACATCGGCGGCGGCAAATCTCATCCGCGAAGGGA
AAACGTGGCAATTGCCCGGCATCATTCAAACCGGTCAACAGGCAGGTATGCAGAATTTTGAGCAGAGCCTGGCCGAGCGG
CGGGCGCAGGGGCGGTTGTTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(102-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0H0A4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.091

100

0.495
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

100

0.495
  pilT Neisseria meningitidis 8013

46.341

100

0.465
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.462
  pilT Legionella pneumophila strain ERS1305867

46.894

98.471

0.462
  pilT Legionella pneumophila strain Lp02

46.894

98.471

0.462
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.459
  pilT Acinetobacter baumannii D1279779

45.122

100

0.453
  pilT Acinetobacter baumannii strain A118

45.122

100

0.453
  pilT Acinetobacter baylyi ADP1

44.817

100

0.45
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.45
  pilT Acinetobacter nosocomialis M2

45.483

98.165

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.433

98.165

0.407
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.376
  pilU Vibrio cholerae strain A1552

39.032

94.801

0.37