Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IC803_RS03740 Genome accession   NZ_CP061474
Coordinates   743841..744896 (+) Length   351 a.a.
NCBI ID   WP_081208505.1    Uniprot ID   A0A1V9BAP5
Organism   Geobacillus sp. 46C-IIa     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738841..749896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC803_RS03725 (IC803_03725) - 740013..740792 (+) 780 WP_081208517.1 PRC-barrel domain-containing protein -
  IC803_RS03730 (IC803_03730) - 740809..742167 (+) 1359 WP_081208513.1 G5 domain-containing protein -
  IC803_RS03735 (IC803_03735) - 742164..743828 (+) 1665 WP_081208509.1 GspE/PulE family protein -
  IC803_RS03740 (IC803_03740) pilT 743841..744896 (+) 1056 WP_081208505.1 type IV pilus twitching motility protein PilT Machinery gene
  IC803_RS03745 (IC803_03745) - 744883..746094 (+) 1212 WP_081208500.1 type II secretion system F family protein -
  IC803_RS03750 (IC803_03750) - 746423..746845 (+) 423 WP_081208498.1 type IV pilin protein -
  IC803_RS03755 (IC803_03755) - 746889..747650 (+) 762 WP_081208496.1 A24 family peptidase -
  IC803_RS03760 (IC803_03760) pilM 747676..748608 (+) 933 WP_081208494.1 type IV pilus biogenesis protein PilM -
  IC803_RS03765 (IC803_03765) - 748598..749173 (+) 576 WP_081208492.1 fimbrial protein -
  IC803_RS03770 (IC803_03770) - 749170..749838 (+) 669 WP_081208490.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38552.67 Da        Isoelectric Point: 7.5779

>NTDB_id=482552 IC803_RS03740 WP_081208505.1 743841..744896(+) (pilT) [Geobacillus sp. 46C-IIa]
MKEKLEALLRAAFECRASDVHLTVGAPPIFRLNGELKAYGHERLRSEETEQMAKAIIPPPLWQQFQEAGELDFSYGIAGV
SRFRFNVFKQRSCVSLAVRLIPLRIPTLEELGMPSVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYMNKTMSKHIITL
EDPIEYLHKHGKSIIDQREIGFDTGSFAAGLRAALRQDPDIILVGEMRDLETIQTAVTAAETGHLVLGTLHTASAPAAIE
RMIDVFPPAQQAQIRLQLASVLVAIIAQRLFPNGRKSGRTAALEILINNAAVANLIRNKKEHQIVNIMQTSRNAGMQTME
MSIKELAADGRIDPLAAEAYLAEERGEHGTI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=482552 IC803_RS03740 WP_081208505.1 743841..744896(+) (pilT) [Geobacillus sp. 46C-IIa]
ATGAAGGAAAAGCTTGAGGCGCTATTGCGCGCCGCCTTTGAATGCCGCGCGTCCGATGTGCATTTAACTGTTGGCGCGCC
GCCGATTTTCCGCTTGAATGGGGAACTGAAGGCATACGGCCATGAGCGGCTTCGCTCGGAAGAGACCGAACAAATGGCAA
AAGCCATCATTCCGCCGCCGCTTTGGCAGCAGTTCCAAGAAGCGGGGGAGCTCGACTTTTCGTACGGCATAGCCGGCGTC
TCGCGCTTCCGTTTCAATGTGTTTAAACAGCGGTCGTGCGTTTCTTTAGCTGTGCGCCTCATTCCGCTGCGCATTCCGAC
GCTTGAGGAGCTCGGCATGCCGAGCGTCTTGAAACGCATCGCCGAAAAGCCGCACGGGCTCGTGCTTGTCACCGGGCCGA
CCGGCAGCGGCAAATCGACGACATTGGCGGCTATGATCGACTATATGAACAAAACGATGTCAAAACATATCATTACGCTC
GAAGACCCAATCGAATATTTGCATAAACACGGAAAATCGATCATCGACCAGCGTGAGATCGGATTTGACACCGGCAGTTT
TGCCGCCGGGCTGCGCGCTGCGCTCCGCCAAGACCCGGATATCATTTTAGTCGGGGAAATGCGCGATTTGGAGACGATTC
AAACGGCGGTCACCGCCGCGGAAACGGGGCATTTAGTGTTAGGCACGCTCCATACAGCGAGCGCGCCGGCGGCGATCGAG
CGGATGATTGACGTCTTTCCGCCGGCTCAGCAGGCACAGATTCGTCTCCAGCTCGCGTCCGTGCTTGTGGCCATCATCGC
CCAGCGTCTGTTTCCAAACGGCCGTAAAAGCGGTCGGACAGCCGCGCTGGAAATTTTAATCAACAATGCGGCCGTTGCCA
ATTTAATCCGCAACAAAAAGGAGCATCAAATCGTCAACATTATGCAGACGAGCCGCAACGCGGGCATGCAGACGATGGAA
ATGAGCATAAAAGAGCTTGCGGCCGACGGACGCATCGATCCGCTTGCCGCCGAGGCGTATTTGGCAGAGGAGCGTGGCGA
GCATGGCACAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V9BAP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.978

90.883

0.481
  pilT Acinetobacter baumannii strain A118

47.734

94.302

0.45
  pilT Acinetobacter baumannii D1279779

47.734

94.302

0.45
  pilT Acinetobacter baylyi ADP1

47.59

94.587

0.45
  pilT Pseudomonas aeruginosa PAK

48.916

92.023

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.16

92.877

0.447
  pilT Vibrio cholerae strain A1552

48.16

92.877

0.447
  pilT Neisseria meningitidis 8013

47.006

95.157

0.447
  pilT Neisseria gonorrhoeae MS11

47.006

95.157

0.447
  pilT Acinetobacter nosocomialis M2

47.432

94.302

0.447
  pilT Pseudomonas stutzeri DSM 10701

47.678

92.023

0.439
  pilT Legionella pneumophila strain Lp02

47.335

90.883

0.43
  pilT Legionella pneumophila strain ERS1305867

47.335

90.883

0.43
  pilU Pseudomonas stutzeri DSM 10701

42.249

93.732

0.396
  pilU Vibrio cholerae strain A1552

41.033

93.732

0.385