Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IAR35_RS03995 Genome accession   NZ_CP060837
Coordinates   817867..818847 (+) Length   326 a.a.
NCBI ID   WP_079981939.1    Uniprot ID   -
Organism   Salmonella enterica strain 852     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 812867..823847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAR35_RS03965 (IAR35_03945) - 813445..814452 (+) 1008 WP_133322457.1 DUF1202 family protein -
  IAR35_RS03970 (IAR35_03950) hemW 814543..815679 (-) 1137 WP_133322459.1 radical SAM family heme chaperone HemW -
  IAR35_RS03975 (IAR35_03955) - 815672..816265 (-) 594 WP_063808830.1 XTP/dITP diphosphatase -
  IAR35_RS03980 (IAR35_03960) yggU 816273..816563 (-) 291 WP_001277204.1 DUF167 family protein YggU -
  IAR35_RS03985 (IAR35_03965) - 816560..817126 (-) 567 WP_001094848.1 YggT family protein -
  IAR35_RS03990 (IAR35_03970) - 817145..817849 (-) 705 WP_079897667.1 YggS family pyridoxal phosphate-dependent enzyme -
  IAR35_RS03995 (IAR35_03975) pilT 817867..818847 (+) 981 WP_079981939.1 type IV pilus twitching motility protein PilT Machinery gene
  IAR35_RS04000 (IAR35_03980) - 818979..819665 (+) 687 WP_023202685.1 IclR family transcriptional regulator -
  IAR35_RS04005 (IAR35_03985) ruvX 819712..820128 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  IAR35_RS04010 (IAR35_03990) - 820128..820691 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  IAR35_RS04015 (IAR35_03995) gshB 820907..821854 (-) 948 WP_000593248.1 glutathione synthase -
  IAR35_RS04020 (IAR35_04000) rsmE 821874..822605 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IAR35_RS04025 (IAR35_04005) endA 822682..823389 (-) 708 WP_133322461.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36156.40 Da        Isoelectric Point: 7.5356

>NTDB_id=478675 IAR35_RS03995 WP_079981939.1 817867..818847(+) (pilT) [Salmonella enterica strain 852]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTMAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=478675 IAR35_RS03995 WP_079981939.1 817867..818847(+) (pilT) [Salmonella enterica strain 852]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTACTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGATGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

35.542

100

0.362