Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H9L34_RS03650 Genome accession   NZ_CP060508
Coordinates   762627..763607 (+) Length   326 a.a.
NCBI ID   WP_001635527.1    Uniprot ID   A0A974QEG8
Organism   Salmonella enterica strain SLR1_7697     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 757627..768607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9L34_RS03620 (H9L34_03620) - 758205..759212 (+) 1008 WP_001635537.1 DUF1202 domain-containing protein -
  H9L34_RS03625 (H9L34_03625) hemW 759303..760439 (-) 1137 WP_023217479.1 radical SAM family heme chaperone HemW -
  H9L34_RS03630 (H9L34_03630) - 760432..761025 (-) 594 WP_023217480.1 XTP/dITP diphosphatase -
  H9L34_RS03635 (H9L34_03635) yggU 761033..761323 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  H9L34_RS03640 (H9L34_03640) - 761320..761886 (-) 567 WP_001094848.1 YggT family protein -
  H9L34_RS03645 (H9L34_03645) - 761905..762609 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  H9L34_RS03650 (H9L34_03650) pilT 762627..763607 (+) 981 WP_001635527.1 type IV pilus twitching motility protein PilT Machinery gene
  H9L34_RS03655 (H9L34_03655) - 763735..764436 (+) 702 WP_023217481.1 IclR family transcriptional regulator -
  H9L34_RS03660 (H9L34_03660) ruvX 764468..764884 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  H9L34_RS03665 (H9L34_03665) - 764884..765447 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  H9L34_RS03670 (H9L34_03670) gshB 765663..766610 (-) 948 WP_000593251.1 glutathione synthase -
  H9L34_RS03675 (H9L34_03675) rsmE 766630..767361 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H9L34_RS03680 (H9L34_03680) endA 767438..768145 (-) 708 WP_000286116.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36154.43 Da        Isoelectric Point: 7.5355

>NTDB_id=476439 H9L34_RS03650 WP_001635527.1 762627..763607(+) (pilT) [Salmonella enterica strain SLR1_7697]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQMPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=476439 H9L34_RS03650 WP_001635527.1 762627..763607(+) (pilT) [Salmonella enterica strain SLR1_7697]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTACGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GATGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGTGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.336

100

0.371