Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H8F24_RS05300 Genome accession   NZ_CP060398
Coordinates   1102511..1103590 (+) Length   359 a.a.
NCBI ID   WP_197157863.1    Uniprot ID   -
Organism   Synechococcus sp. CBW1002     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1097511..1108590
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F24_RS05280 (H8F24_05275) - 1098381..1098668 (-) 288 WP_370594671.1 sulfurtransferase TusA family protein -
  H8F24_RS05285 (H8F24_05280) dnaJ 1098677..1099798 (-) 1122 WP_197157867.1 molecular chaperone DnaJ -
  H8F24_RS05290 (H8F24_05285) grpE 1099798..1100490 (-) 693 WP_231598115.1 nucleotide exchange factor GrpE -
  H8F24_RS05295 (H8F24_05290) - 1100591..1102501 (+) 1911 WP_197157865.1 GspE/PulE family protein -
  H8F24_RS05300 (H8F24_05295) pilT 1102511..1103590 (+) 1080 WP_197157863.1 type IV pilus twitching motility protein PilT Machinery gene
  H8F24_RS05305 (H8F24_05300) - 1103611..1104873 (+) 1263 WP_197157861.1 type II secretion system F family protein -
  H8F24_RS05310 (H8F24_05305) - 1105076..1106029 (+) 954 WP_197171117.1 IS1595 family transposase -
  H8F24_RS05315 (H8F24_05310) - 1106215..1107483 (-) 1269 WP_197158986.1 NAD(P)/FAD-dependent oxidoreductase -
  H8F24_RS05320 (H8F24_05315) dusA 1107490..1108530 (+) 1041 WP_197157859.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39922.83 Da        Isoelectric Point: 5.9369

>NTDB_id=475710 H8F24_RS05300 WP_197157863.1 1102511..1103590(+) (pilT) [Synechococcus sp. CBW1002]
MDLMIEDLMGKLVEDGGSDLHLSAGMPPYGRFNGQLRPMTETALKEEDCNRLIFMLLNNAQRKQLEQTWELDCSYGLKGV
SRFRVNVYRQRGTYAACLRALGNKIPTLDMLGLPPIVEEMSRIPKGLVLVTGPTGSGKTTTLAAILDHINHTRAEHILTI
EDPIEFTYRSDQSIIHQRELSQDTRSFANALRAALREDPDVILVGEMRDLETIQLAVTAAETGHLVFGTLHTSSASQTVD
RMVDVFPAGQQDQIRVQLSSSLMAVFSQTLCRRWNPKPGQFGRVMAQEIMINTPAISNLVREGKTAQLYSQMQTGASLGM
QTLEKALANLVLSEEVKLEEALSKTAKPEELRRLVDQLS

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=475710 H8F24_RS05300 WP_197157863.1 1102511..1103590(+) (pilT) [Synechococcus sp. CBW1002]
ATGGACCTGATGATCGAGGATCTGATGGGCAAACTGGTTGAAGATGGCGGTAGTGATCTTCACCTCAGTGCCGGGATGCC
TCCTTACGGACGCTTCAATGGCCAGCTTCGTCCGATGACGGAGACAGCGTTGAAGGAGGAAGACTGCAACAGACTGATCT
TCATGTTGCTGAACAACGCCCAGCGCAAGCAACTGGAGCAAACCTGGGAACTGGACTGCTCCTATGGCCTCAAAGGCGTC
TCCCGATTCCGGGTGAACGTCTATCGCCAACGCGGCACCTACGCCGCTTGCCTGAGAGCTCTTGGCAACAAGATTCCCAC
TCTGGACATGCTTGGACTTCCACCGATTGTGGAAGAGATGAGTCGTATCCCCAAAGGGCTGGTACTGGTAACCGGTCCGA
CAGGCTCCGGAAAAACCACCACTCTGGCGGCAATTCTGGATCACATCAACCACACCCGAGCTGAGCATATCCTCACGATC
GAGGATCCGATCGAGTTCACCTACCGTTCCGATCAAAGCATCATCCACCAACGTGAGCTCAGTCAAGACACCCGCAGTTT
CGCCAATGCCCTGCGGGCAGCTCTTCGCGAAGATCCGGACGTGATCCTGGTTGGTGAGATGCGGGACCTGGAGACGATCC
AACTGGCAGTGACCGCAGCCGAAACTGGGCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCCTCCCAGACGGTGGAT
CGGATGGTGGACGTTTTCCCGGCGGGCCAGCAGGATCAGATCAGGGTGCAACTCAGCAGCAGCCTGATGGCTGTGTTTTC
CCAGACTCTCTGCAGGCGCTGGAATCCGAAACCCGGCCAATTCGGCCGGGTGATGGCTCAGGAGATCATGATCAACACTC
CTGCCATCTCCAATCTGGTGCGCGAAGGAAAAACTGCCCAGCTTTATTCCCAGATGCAGACCGGGGCCAGCCTCGGCATG
CAGACCCTGGAGAAGGCCCTGGCCAATCTGGTGCTTTCCGAGGAGGTGAAACTGGAGGAGGCTCTCAGCAAGACTGCCAA
ACCGGAGGAACTACGCCGCCTGGTGGATCAGCTTTCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.45

96.657

0.507
  pilT Acinetobacter baumannii strain A118

52.161

96.657

0.504
  pilT Acinetobacter nosocomialis M2

52.161

96.657

0.504
  pilT Acinetobacter baumannii D1279779

52.161

96.657

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.585

96.657

0.499
  pilT Vibrio cholerae strain A1552

52.647

94.708

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.647

94.708

0.499
  pilT Acinetobacter baylyi ADP1

51.297

96.657

0.496
  pilT Legionella pneumophila strain ERS1305867

51.594

96.1

0.496
  pilT Legionella pneumophila strain Lp02

51.594

96.1

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.296

98.886

0.487
  pilT Neisseria meningitidis 8013

51.064

91.643

0.468
  pilT Neisseria gonorrhoeae MS11

50.76

91.643

0.465
  pilU Pseudomonas stutzeri DSM 10701

39.031

97.772

0.382
  pilU Acinetobacter baylyi ADP1

38.109

97.214

0.37