Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H8F25_RS11925 Genome accession   NZ_CP060397
Coordinates   2493976..2495055 (+) Length   359 a.a.
NCBI ID   WP_304623255.1    Uniprot ID   -
Organism   Synechococcus sp. CBW1004     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488976..2500055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F25_RS11900 (H8F25_11890) rsgA 2488994..2490124 (-) 1131 WP_197210597.1 ribosome small subunit-dependent GTPase A -
  H8F25_RS11905 (H8F25_11895) - 2490117..2490377 (-) 261 WP_197210598.1 sulfurtransferase TusA family protein -
  H8F25_RS11910 (H8F25_11900) dnaJ 2490374..2491498 (-) 1125 WP_197210599.1 molecular chaperone DnaJ -
  H8F25_RS11915 (H8F25_11905) grpE 2491498..2492175 (-) 678 WP_197210600.1 nucleotide exchange factor GrpE -
  H8F25_RS11920 (H8F25_11910) pilB/pilB1 2492392..2493846 (+) 1455 WP_197210601.1 GspE/PulE family protein Machinery gene
  H8F25_RS11925 (H8F25_11915) pilT 2493976..2495055 (+) 1080 WP_304623255.1 type IV pilus twitching motility protein PilT Machinery gene
  H8F25_RS11930 (H8F25_11920) - 2495079..2496311 (+) 1233 WP_197210602.1 type II secretion system F family protein -
  H8F25_RS11935 (H8F25_11925) - 2496316..2497566 (-) 1251 WP_370525733.1 NAD(P)/FAD-dependent oxidoreductase -
  H8F25_RS11940 (H8F25_11930) dusA 2497565..2498590 (+) 1026 WP_197210604.1 tRNA dihydrouridine(20/20a) synthase DusA -
  H8F25_RS11945 (H8F25_11935) - 2498623..2499105 (-) 483 WP_197210605.1 RNA-binding protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39568.27 Da        Isoelectric Point: 6.6090

>NTDB_id=475703 H8F25_RS11925 WP_304623255.1 2493976..2495055(+) (pilT) [Synechococcus sp. CBW1004]
MDLQIEDLMIELVNGGGSDLHLAAGQPPFGRFNGQLRPILPDLFLSEEQCNKLIFSLLNNAQRKQLEQTWELDCSYGLKD
VARFRVNVYRQRGTYAACLRALGNSIPPMEKLGMPPIVEEMSRKPKGLVLVTGPTGSGKTTTLAAMLDHINQTRSEHILT
IEDPIEFTYKNQKSLIHQREIGEDTRSFANALRAALREDPDVVLVGEMRDLETIQLAITAAETGHLVFGTLHTSSASQTV
DRMVDVFPPSQQTQIRVQLSGSLLAVFSQTLCRSSHPSTGAFGRVMAQEIMINTPAISNLIREGKTAQIYSQIQTGANLG
MQTLERALANLVTAGKVSRSEALSKTTKPEELTRLLDQN

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=475703 H8F25_RS11925 WP_304623255.1 2493976..2495055(+) (pilT) [Synechococcus sp. CBW1004]
GTGGATCTTCAGATCGAAGACCTGATGATTGAGCTGGTGAATGGCGGGGGCAGCGACCTGCACCTGGCCGCCGGACAACC
GCCGTTCGGTCGGTTCAACGGCCAGCTCCGACCGATCCTTCCGGATCTGTTTCTGAGTGAGGAACAATGCAACAAGCTTA
TTTTTTCTCTGCTCAACAACGCGCAACGCAAGCAGCTTGAGCAGACCTGGGAACTGGATTGCTCCTACGGCCTCAAGGAT
GTTGCCCGTTTCCGAGTGAATGTGTATCGGCAGCGTGGCACCTATGCCGCCTGTCTGCGGGCGCTTGGCAACTCGATCCC
ACCGATGGAGAAGCTGGGCATGCCGCCGATCGTGGAGGAGATGAGCCGCAAGCCGAAAGGGCTGGTGCTGGTCACCGGGC
CGACGGGCTCAGGCAAGACCACCACGCTGGCCGCGATGCTGGATCACATCAATCAAACCCGATCCGAGCATATCCTCACC
ATCGAGGATCCGATCGAATTCACCTATAAGAATCAAAAGAGTCTCATCCACCAGCGCGAGATCGGTGAGGACACGCGCAG
CTTCGCCAATGCCCTGCGCGCGGCCCTGCGGGAGGATCCTGATGTGGTTCTGGTCGGGGAAATGCGCGACCTGGAGACGA
TTCAGCTGGCCATCACCGCGGCCGAAACCGGCCACCTGGTCTTCGGTACCCTGCACACCAGCTCCGCCTCCCAGACGGTC
GACCGCATGGTGGACGTGTTTCCACCGAGCCAGCAGACCCAGATCCGCGTGCAGCTGAGCGGCAGCCTTCTGGCGGTGTT
CTCCCAGACCCTCTGTCGCAGCAGTCATCCCAGCACGGGAGCCTTTGGACGGGTGATGGCTCAGGAAATCATGATCAACA
CCCCCGCCATCAGCAATCTGATCCGCGAAGGCAAGACCGCACAGATCTATTCCCAGATTCAGACCGGTGCGAACCTGGGG
ATGCAGACACTCGAGCGGGCCCTCGCCAATCTCGTGACGGCAGGCAAGGTGTCACGCAGCGAGGCACTCAGCAAAACCAC
CAAACCCGAAGAGCTGACCCGGCTGCTCGATCAGAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(82-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.131

97.772

0.529
  pilT Pseudomonas stutzeri DSM 10701

53.276

97.772

0.521
  pilT Acinetobacter nosocomialis M2

52.137

97.772

0.51
  pilT Acinetobacter baumannii D1279779

52.137

97.772

0.51
  pilT Acinetobacter baumannii strain A118

52.137

97.772

0.51
  pilT Acinetobacter baylyi ADP1

51.852

97.772

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.77

95.543

0.504
  pilT Vibrio cholerae strain A1552

52.77

95.543

0.504
  pilT Legionella pneumophila strain ERS1305867

52.493

94.986

0.499
  pilT Legionella pneumophila strain Lp02

52.493

94.986

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.859

98.886

0.493
  pilT Neisseria meningitidis 8013

50

98.05

0.49
  pilT Neisseria gonorrhoeae MS11

49.716

98.05

0.487
  pilU Acinetobacter baylyi ADP1

37.11

98.329

0.365