Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   H8F25_RS11920 Genome accession   NZ_CP060397
Coordinates   2492392..2493846 (+) Length   484 a.a.
NCBI ID   WP_197210601.1    Uniprot ID   A0A7T1HVE4
Organism   Synechococcus sp. CBW1004     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2487392..2498846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H8F25_RS11890 (H8F25_11880) murB 2487728..2488669 (+) 942 WP_197210595.1 UDP-N-acetylmuramate dehydrogenase -
  H8F25_RS11895 (H8F25_11885) - 2488696..2489037 (+) 342 WP_197210596.1 YbaB/EbfC family nucleoid-associated protein -
  H8F25_RS11900 (H8F25_11890) rsgA 2488994..2490124 (-) 1131 WP_197210597.1 ribosome small subunit-dependent GTPase A -
  H8F25_RS11905 (H8F25_11895) - 2490117..2490377 (-) 261 WP_197210598.1 sulfurtransferase TusA family protein -
  H8F25_RS11910 (H8F25_11900) dnaJ 2490374..2491498 (-) 1125 WP_197210599.1 molecular chaperone DnaJ -
  H8F25_RS11915 (H8F25_11905) grpE 2491498..2492175 (-) 678 WP_197210600.1 nucleotide exchange factor GrpE -
  H8F25_RS11920 (H8F25_11910) pilB/pilB1 2492392..2493846 (+) 1455 WP_197210601.1 GspE/PulE family protein Machinery gene
  H8F25_RS11925 (H8F25_11915) pilT 2493976..2495055 (+) 1080 WP_304623255.1 type IV pilus twitching motility protein PilT Machinery gene
  H8F25_RS11930 (H8F25_11920) - 2495079..2496311 (+) 1233 WP_197210602.1 type II secretion system F family protein -
  H8F25_RS11935 (H8F25_11925) - 2496316..2497566 (-) 1251 WP_370525733.1 NAD(P)/FAD-dependent oxidoreductase -
  H8F25_RS11940 (H8F25_11930) dusA 2497565..2498590 (+) 1026 WP_197210604.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 53244.49 Da        Isoelectric Point: 4.8452

>NTDB_id=475702 H8F25_RS11920 WP_197210601.1 2492392..2493846(+) (pilB/pilB1) [Synechococcus sp. CBW1004]
MTPEATSPLTQGATAERSSLFSDLDLDIARVPQSEPPNPEEDLEASSQAALSPVMSLVDRILIEALTNAASDIHIEPQEE
GLQIRFRQDGVLRIVDTLPRTLIPAVTSRLKIMADLDIAERRMPQDGRIRRNFRGRIMDFRVSTLPSRYGEKVVMRLLDS
GATQLGLDTLITDATSRETLRDLGSKPFGMILVTGPTGSGKSTTLYALLAERNEPGINISTVEDPIEYTLHGITQTQVNR
EKGYDFSTALRAFMRQDPDVLLVGETRDLETARTAIEAALTGHLVLTTLHCNDAASAFARLDEMGVEPFLVSASLLGVIS
QRLVRRLCGDCREPYHPDSEELAHFGLVATDEQRITFYRATKSAPGSDGCCPTCQGRGYAGRVGVYEILHMNESLSSAVA
KRSNTEDLRRLALESGMKTLLGYGLDLVREGLTTLEEVERMLLTDTGLESERRARALNTLTCKGCGAGLQDEWLECPYCL
RLRS

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=475702 H8F25_RS11920 WP_197210601.1 2492392..2493846(+) (pilB/pilB1) [Synechococcus sp. CBW1004]
ATGACGCCAGAAGCCACCTCGCCGCTGACCCAGGGCGCAACGGCCGAACGGTCCTCTCTGTTCAGCGATCTGGATCTGGA
CATCGCGCGGGTGCCCCAGTCCGAGCCTCCCAATCCGGAGGAAGACCTGGAAGCAAGCAGCCAGGCCGCTCTCTCCCCGG
TGATGAGCCTGGTGGACCGGATCCTGATCGAGGCGCTCACCAACGCCGCCAGCGACATCCATATCGAACCCCAGGAGGAG
GGGCTGCAGATCCGCTTCCGGCAGGACGGGGTGCTCCGCATCGTGGACACGCTGCCACGGACGCTGATCCCGGCCGTCAC
CTCACGCCTCAAGATCATGGCCGACCTGGACATCGCCGAGCGGCGGATGCCCCAGGACGGGCGCATCCGCCGCAACTTCC
GTGGCCGCATCATGGACTTCCGCGTCAGCACGCTGCCGAGTCGCTACGGCGAGAAGGTGGTGATGCGACTGCTCGACAGC
GGCGCCACGCAGCTGGGCCTGGACACGCTGATCACCGACGCGACATCTCGCGAGACCCTGAGGGACCTGGGATCCAAGCC
GTTCGGGATGATCCTGGTGACCGGCCCCACCGGCTCAGGCAAATCGACCACGCTCTATGCACTGCTGGCGGAGCGCAACG
AGCCCGGCATCAACATCTCCACGGTGGAGGATCCGATCGAATACACCCTGCACGGCATCACCCAGACCCAGGTGAACCGT
GAAAAGGGCTACGACTTCAGCACGGCCCTGCGAGCCTTCATGCGCCAGGATCCGGATGTGCTGCTCGTCGGCGAGACGCG
CGACCTGGAGACGGCGCGCACGGCGATCGAAGCCGCCCTCACCGGCCACCTGGTGCTGACCACCCTGCACTGCAACGATG
CCGCCAGCGCCTTCGCGCGGTTGGATGAGATGGGGGTGGAGCCCTTTCTGGTGAGCGCCTCCCTGCTGGGCGTGATCTCC
CAACGGCTGGTCCGCCGTCTGTGCGGCGACTGCCGGGAGCCTTACCACCCCGACAGTGAGGAGCTGGCCCATTTCGGCCT
GGTGGCCACGGACGAGCAGCGCATCACCTTCTATCGCGCCACCAAGAGCGCGCCGGGCAGCGACGGCTGCTGTCCCACCT
GTCAGGGCCGCGGCTACGCCGGCCGGGTCGGCGTGTACGAGATCCTGCACATGAACGAATCGCTGTCCTCGGCGGTCGCC
AAACGCAGCAACACCGAGGATCTGCGCCGCCTGGCGCTGGAGAGCGGCATGAAGACGCTGCTGGGCTACGGCCTCGATCT
GGTGCGCGAGGGCCTGACCACCCTTGAGGAGGTCGAGAGGATGCTGCTGACCGACACGGGCCTGGAATCGGAGCGGCGCG
CCCGCGCCCTCAACACGCTGACCTGCAAAGGCTGCGGCGCCGGCCTTCAGGACGAGTGGCTCGAGTGTCCCTACTGCCTA
CGGCTGCGGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T1HVE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

61.659

92.149

0.568
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.524

86.777

0.386
  pilF Thermus thermophilus HB27

46.059

83.884

0.386
  pilB Vibrio campbellii strain DS40M4

45.409

83.264

0.378
  pilB Legionella pneumophila strain ERS1305867

44.691

83.678

0.374
  pilB Vibrio cholerae strain A1552

45.918

80.992

0.372
  pilB Vibrio parahaemolyticus RIMD 2210633

45.408

80.992

0.368
  pilB Acinetobacter baumannii D1279779

40.835

89.05

0.364