Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H5J27_RS26975 Genome accession   NZ_CP059995
Coordinates   5690701..5691156 (-) Length   151 a.a.
NCBI ID   WP_033976389.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY3045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5685701..5696156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS26955 (H5J27_26955) ampD 5686407..5686973 (-) 567 WP_039028111.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H5J27_RS26960 (H5J27_26960) - 5687123..5689378 (-) 2256 WP_033976391.1 DUF1631 domain-containing protein -
  H5J27_RS26965 (H5J27_26965) nadC 5689664..5690512 (+) 849 WP_033976390.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H5J27_RS26975 (H5J27_26975) pilA 5690701..5691156 (-) 456 WP_033976389.1 pilin Machinery gene
  H5J27_RS26980 (H5J27_26980) pilB 5691388..5693088 (+) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H5J27_RS26985 (H5J27_26985) pilC 5693092..5694312 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  H5J27_RS26990 (H5J27_26990) pilD 5694316..5695188 (+) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  H5J27_RS26995 (H5J27_26995) coaE 5695185..5695796 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  H5J27_RS27000 (H5J27_27000) yacG 5695793..5695993 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15862.20 Da        Isoelectric Point: 7.7840

>NTDB_id=473126 H5J27_RS26975 WP_033976389.1 5690701..5691156(-) (pilA) [Pseudomonas aeruginosa strain NY3045]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASINPLKTTVEEALSRGWSVRSGTGAEDAAKKEVPLG
VDGKANNLGTIALNPDPANGAADITLTFTMDAAGPKNKDKVITLTRTATDGLWKCTSDQEKQFIPKGCSDK

Nucleotide


Download         Length: 456 bp        

>NTDB_id=473126 H5J27_RS26975 WP_033976389.1 5690701..5691156(-) (pilA) [Pseudomonas aeruginosa strain NY3045]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAATACCAGAACTATGTTGCGCGCTCGGAAGGCGCTTCGGCGCTGGCGTCCATCAACCCGTTGAAGACAACTGTAG
AAGAGGCTCTTTCTCGTGGTTGGAGCGTTAGGAGTGGTACTGGTGCAGAAGACGCTGCTAAAAAAGAAGTTCCGCTGGGT
GTAGATGGAAAGGCCAATAATCTCGGCACCATTGCTCTGAACCCTGATCCTGCAAATGGTGCTGCTGATATTACTCTGAC
CTTCACCATGGATGCTGCTGGTCCGAAGAACAAAGACAAAGTCATTACTCTGACTCGTACTGCTACTGATGGCCTTTGGA
AGTGCACCAGTGATCAGGAAAAGCAGTTCATTCCGAAAGGCTGCTCTGACAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

87.919

98.675

0.868
  pilA Acinetobacter baumannii strain A118

44.371

100

0.444
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44

99.338

0.437
  pilA Vibrio cholerae strain A1552

44

99.338

0.437
  pilA Vibrio cholerae C6706

44

99.338

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.421

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.205

96.689

0.437
  pilA Vibrio parahaemolyticus RIMD 2210633

47.761

88.742

0.424
  comP Acinetobacter baylyi ADP1

41.333

99.338

0.411
  pilA2 Legionella pneumophila str. Paris

40.136

97.351

0.391
  pilA2 Legionella pneumophila strain ERS1305867

39.456

97.351

0.384
  pilA/pilA1 Eikenella corrodens VA1

36.25

100

0.384
  pilA Acinetobacter nosocomialis M2

46.61

78.146

0.364