Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H5J27_RS26980 Genome accession   NZ_CP059995
Coordinates   5691388..5693088 (+) Length   566 a.a.
NCBI ID   WP_033976388.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY3045     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5686388..5698088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5J27_RS26955 (H5J27_26955) ampD 5686407..5686973 (-) 567 WP_039028111.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H5J27_RS26960 (H5J27_26960) - 5687123..5689378 (-) 2256 WP_033976391.1 DUF1631 domain-containing protein -
  H5J27_RS26965 (H5J27_26965) nadC 5689664..5690512 (+) 849 WP_033976390.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H5J27_RS26975 (H5J27_26975) pilA 5690701..5691156 (-) 456 WP_033976389.1 pilin Machinery gene
  H5J27_RS26980 (H5J27_26980) pilB 5691388..5693088 (+) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H5J27_RS26985 (H5J27_26985) pilC 5693092..5694312 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  H5J27_RS26990 (H5J27_26990) pilD 5694316..5695188 (+) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  H5J27_RS26995 (H5J27_26995) coaE 5695185..5695796 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  H5J27_RS27000 (H5J27_27000) yacG 5695793..5695993 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  H5J27_RS27005 (H5J27_27005) - 5696030..5696239 (-) 210 WP_003094660.1 hypothetical protein -
  H5J27_RS27010 (H5J27_27010) - 5696345..5697034 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  H5J27_RS27015 (H5J27_27015) - 5697031..5697501 (-) 471 WP_033970459.1 hypothetical protein -
  H5J27_RS27020 (H5J27_27020) - 5697498..5697923 (-) 426 WP_033976385.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62311.21 Da        Isoelectric Point: 5.7028

>NTDB_id=473127 H5J27_RS26980 WP_033976388.1 5691388..5693088(+) (pilB) [Pseudomonas aeruginosa strain NY3045]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLALVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=473127 H5J27_RS26980 WP_033976388.1 5691388..5693088(+) (pilB) [Pseudomonas aeruginosa strain NY3045]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAAACCGCCCTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGGCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAGAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTGGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGCTCGCCAGCCGCATCTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
TATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCTTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCTATCAAGGCGGCACAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.867

100

0.585
  pilB Acinetobacter baylyi ADP1

57.194

99.47

0.569
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio campbellii strain DS40M4

49.291

99.647

0.491
  pilB Vibrio cholerae strain A1552

52.767

89.399

0.472
  pilF Thermus thermophilus HB27

40.253

97.88

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

38.951

94.346

0.367