Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   IXO685_RS17695 Genome accession   NZ_CP059590
Coordinates   3714188..3714613 (+) Length   141 a.a.
NCBI ID   WP_011408068.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain IXO685     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3712574..3727402 3714188..3714613 within 0


Gene organization within MGE regions


Location: 3712574..3727402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IXO685_RS17690 (IXO685_0017685) pilC 3712574..3713833 (-) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene
  IXO685_RS17695 (IXO685_0017690) pilA2 3714188..3714613 (+) 426 WP_011408068.1 pilin Machinery gene
  IXO685_RS17700 (IXO685_0017695) - 3714748..3716337 (+) 1590 WP_075244420.1 phosphoethanolamine transferase -
  IXO685_RS17705 (IXO685_0017700) - 3716408..3716977 (+) 570 WP_229001934.1 hypothetical protein -
  IXO685_RS17710 (IXO685_0017705) - 3716947..3718473 (+) 1527 WP_041182621.1 membrane protein -
  IXO685_RS17715 (IXO685_0017710) pilB 3718508..3720241 (+) 1734 WP_011408072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IXO685_RS17720 (IXO685_0017715) - 3720250..3720597 (-) 348 WP_305954854.1 Fic family protein -
  IXO685_RS17725 - 3720598..3720759 (-) 162 WP_153296778.1 hypothetical protein -
  IXO685_RS17730 (IXO685_0017720) - 3720821..3721027 (-) 207 WP_027703281.1 YhfG family protein -
  IXO685_RS17735 (IXO685_0017725) pilR 3721100..3722551 (-) 1452 WP_011258365.1 sigma-54 dependent transcriptional regulator Regulator
  IXO685_RS17740 (IXO685_0017730) - 3722822..3724435 (-) 1614 WP_012444444.1 HAMP domain-containing sensor histidine kinase -
  IXO685_RS17745 (IXO685_0017735) sucC 3724669..3725838 (+) 1170 WP_011258367.1 ADP-forming succinate--CoA ligase subunit beta -
  IXO685_RS17750 (IXO685_0017740) sucD 3725863..3726738 (+) 876 WP_012444445.1 succinate--CoA ligase subunit alpha -
  IXO685_RS17755 (IXO685_0017745) - 3726911..3727402 (+) 492 WP_027703282.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14442.76 Da        Isoelectric Point: 9.0090

>NTDB_id=471141 IXO685_RS17695 WP_011408068.1 3714188..3714613(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain IXO685]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGVANVTLANSGYSFPGATK
YVSNVTIADGTGLVTVTSTVPNAAGTILLTPKDVGGGQLKWTCSSAILTKYLPAECRSSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=471141 IXO685_RS17695 WP_011408068.1 3714188..3714613(+) (pilA2) [Xanthomonas oryzae pv. oryzae strain IXO685]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCTGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGTGCCAAGCTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAACTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTGTTGCTAATGTTACTCTCGCGAATTCGGGCTACTCATTCCCGGGTGCCACGAAG
TATGTCAGCAACGTCACCATCGCCGATGGTACGGGCCTAGTCACTGTAACTTCTACCGTGCCGAACGCAGCCGGTACCAT
CCTGCTGACCCCGAAGGATGTCGGTGGCGGCCAGCTGAAGTGGACCTGCTCTTCCGCCATCCTCACCAAGTACCTGCCGG
CGGAGTGCCGTAGCTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.471

96.454

0.496
  pilA2 Legionella pneumophila strain ERS1305867

51.471

96.454

0.496
  pilA Ralstonia pseudosolanacearum GMI1000

41.818

100

0.489
  comP Acinetobacter baylyi ADP1

43.537

100

0.454
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.054

100

0.447
  pilE Neisseria gonorrhoeae strain FA1090

37.278

100

0.447
  pilE Neisseria gonorrhoeae MS11

37.5

100

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.446

98.582

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

95.745

0.404
  pilA/pilA1 Eikenella corrodens VA1

37.333

100

0.397
  pilA Acinetobacter baumannii strain A118

38.298

100

0.383
  pilA Pseudomonas aeruginosa PAK

36

100

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

40.157

90.071

0.362