Detailed information NTG0471142

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: IXO685_RS17715
• Genome accession: NZ_CP059590
• Coordinates: 3718508..3720241 (+)
• Length: 577 a.a.
• NCBI ID: WP_011408072.1
• Uniprot ID: A0A0K0GIN1
• Organism: Xanthomonas oryzae pv. oryzae strain IXO685
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3712574..3727402	3718508..3720241	within	0

Gene organization within MGE regions

Location: 3712574..3727402

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IXO685_RS17690 (IXO685_0017685)	pilC	3712574..3713833 (-)	1260	WP_011408067.1	type II secretion system F family protein	Machinery gene
IXO685_RS17695 (IXO685_0017690)	pilA2	3714188..3714613 (+)	426	WP_011408068.1	pilin	Machinery gene
IXO685_RS17700 (IXO685_0017695)	-	3714748..3716337 (+)	1590	WP_075244420.1	phosphoethanolamine transferase	-
IXO685_RS17705 (IXO685_0017700)	-	3716408..3716977 (+)	570	WP_229001934.1	hypothetical protein	-
IXO685_RS17710 (IXO685_0017705)	-	3716947..3718473 (+)	1527	WP_041182621.1	membrane protein	-
IXO685_RS17715 (IXO685_0017710)	pilB	3718508..3720241 (+)	1734	WP_011408072.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IXO685_RS17720 (IXO685_0017715)	-	3720250..3720597 (-)	348	WP_305954854.1	Fic family protein	-
IXO685_RS17725	-	3720598..3720759 (-)	162	WP_153296778.1	hypothetical protein	-
IXO685_RS17730 (IXO685_0017720)	-	3720821..3721027 (-)	207	WP_027703281.1	YhfG family protein	-
IXO685_RS17735 (IXO685_0017725)	pilR	3721100..3722551 (-)	1452	WP_011258365.1	sigma-54 dependent transcriptional regulator	Regulator
IXO685_RS17740 (IXO685_0017730)	-	3722822..3724435 (-)	1614	WP_012444444.1	HAMP domain-containing sensor histidine kinase	-
IXO685_RS17745 (IXO685_0017735)	sucC	3724669..3725838 (+)	1170	WP_011258367.1	ADP-forming succinate--CoA ligase subunit beta	-
IXO685_RS17750 (IXO685_0017740)	sucD	3725863..3726738 (+)	876	WP_012444445.1	succinate--CoA ligase subunit alpha	-
IXO685_RS17755 (IXO685_0017745)	-	3726911..3727402 (+)	492	WP_027703282.1	hypothetical protein	-

Sequence

Protein

• Length: 577 a.a.
• Molecular weight: 62970.00 Da
• Isoelectric Point: 5.4513

>NTDB_id=471142 IXO685_RS17715 WP_011408072.1 3718508..3720241(+) (pilB) [Xanthomonas oryzae pv. oryzae strain IXO685]
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide

• Length: 1734 bp

>NTDB_id=471142 IXO685_RS17715 WP_011408072.1 3718508..3720241(+) (pilB) [Xanthomonas oryzae pv. oryzae strain IXO685]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AACCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAACAGGCTGG
TGTCGGCGTCGCAGCTTGCGGCGGCCAATGCAGTGGAATTTGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTTGATGACATCAAGTTTCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATCCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTTGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-152)

3D structure

• AlphaFold DB: A0A0K0GIN1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	57.042	98.44	0.562
pilB	Acinetobacter baumannii D1279779	56.69	98.44	0.558
pilB	Legionella pneumophila strain ERS1305867	52.465	98.44	0.516
pilB	Vibrio cholerae strain A1552	49.74	100	0.497
pilF	Neisseria gonorrhoeae MS11	49.648	98.44	0.489
pilB	Vibrio campbellii strain DS40M4	46.046	98.614	0.454
pilB	Vibrio parahaemolyticus RIMD 2210633	46.359	97.574	0.452
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.394	91.508	0.36