Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H1R18_RS05045 Genome accession   NZ_CP059481
Coordinates   841075..842055 (+) Length   326 a.a.
NCBI ID   WP_045281449.1    Uniprot ID   -
Organism   Enterobacter kobei strain IB2020     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 836075..847055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1R18_RS05005 (H1R18_05005) - 836143..836469 (+) 327 WP_014885198.1 YggL family protein -
  H1R18_RS05010 (H1R18_05010) - 836526..837242 (+) 717 WP_014885197.1 DUF2884 domain-containing protein -
  H1R18_RS05015 (H1R18_05015) - 837317..837679 (-) 363 WP_014885196.1 endonuclease domain-containing protein -
  H1R18_RS05020 (H1R18_05020) hemW 837743..838885 (-) 1143 WP_023338778.1 radical SAM family heme chaperone HemW -
  H1R18_RS05025 (H1R18_05025) - 838878..839471 (-) 594 WP_050861951.1 XTP/dITP diphosphatase -
  H1R18_RS05030 (H1R18_05030) yggU 839475..839771 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  H1R18_RS05035 (H1R18_05035) - 839768..840334 (-) 567 WP_014885192.1 YggT family protein -
  H1R18_RS05040 (H1R18_05040) - 840356..841057 (-) 702 WP_014885191.1 YggS family pyridoxal phosphate-dependent enzyme -
  H1R18_RS05045 (H1R18_05045) pilT 841075..842055 (+) 981 WP_045281449.1 type IV pilus twitching motility protein PilT Machinery gene
  H1R18_RS05050 (H1R18_05050) ruvX 842066..842482 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  H1R18_RS05055 (H1R18_05055) - 842482..843042 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  H1R18_RS05060 (H1R18_05060) gshB 843117..844064 (-) 948 WP_023331278.1 glutathione synthase -
  H1R18_RS05065 (H1R18_05065) rsmE 844084..844815 (-) 732 WP_023331277.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H1R18_RS05070 (H1R18_05070) endA 844867..845574 (-) 708 WP_014885187.1 deoxyribonuclease I -
  H1R18_RS05075 (H1R18_05075) - 845669..846166 (-) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35620.59 Da        Isoelectric Point: 6.4113

>NTDB_id=469975 H1R18_RS05045 WP_045281449.1 841075..842055(+) (pilT) [Enterobacter kobei strain IB2020]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGTLLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSVAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=469975 H1R18_RS05045 WP_045281449.1 841075..842055(+) (pilT) [Enterobacter kobei strain IB2020]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCTGATGTCGGGACGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGTGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTCGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAATGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCGGCGATGGTCGATTTCCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAGATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGAGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACCTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAGAGCGTGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

46.041

100

0.482
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38