Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H0A61_RS14930 Genome accession   NZ_CP059066
Coordinates   3039686..3040738 (-) Length   350 a.a.
NCBI ID   WP_206707879.1    Uniprot ID   A0A8A0RQC2
Organism   Koleobacter methoxysyntrophicus strain NRmbB1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3034686..3045738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0A61_RS14915 (H0A61_02978) tnpA 3035979..3036407 (-) 429 WP_206707862.1 IS200/IS605 family transposase -
  H0A61_RS14920 (H0A61_02979) - 3036503..3037597 (+) 1095 WP_206707877.1 hypothetical protein -
  H0A61_RS14925 (H0A61_02980) - 3037818..3039599 (-) 1782 WP_206707878.1 GspE/PulE family protein -
  H0A61_RS14930 (H0A61_02981) pilT 3039686..3040738 (-) 1053 WP_206707879.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H0A61_RS14935 (H0A61_02982) - 3040816..3042063 (-) 1248 WP_241754917.1 GLUG motif-containing protein -
  H0A61_RS14940 (H0A61_02983) - 3042349..3043092 (-) 744 WP_206707881.1 M23 family metallopeptidase -
  H0A61_RS14945 (H0A61_02984) - 3043112..3044857 (-) 1746 WP_206707882.1 type II secretion system F family protein -
  H0A61_RS14950 (H0A61_02985) - 3044802..3045407 (-) 606 WP_241755024.1 type II secretion system protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38936.65 Da        Isoelectric Point: 7.0604

>NTDB_id=466958 H0A61_RS14930 WP_206707879.1 3039686..3040738(-) (pilT) [Koleobacter methoxysyntrophicus strain NRmbB1]
MITDIIKSALDQKASDIHLTEGLPPVFRINGRLAVQRQFDVFSAGALAETAKQLLGENYENYGQKRSFDFSFHAEGRRFR
GHVFKQRGADAVVLRVIPAEVPTVDQLLLPESVKKFTSLRDGLVLVTGPTGSGKSTTLAALINEINRAQEKHIITIEDPV
EFIHPHRRCIVNQREVGTDVENFADAVRSAMREDPDILLVGEMRDLETIQNAITMAETGHLVFATLHTRSAAETIDRIID
VFPPVQQQQIRVQLSSILQGIISQCLVPAVSGGRVPVCEVMFATDGIKSIIKENGPHSSIKDQILLNHRKLGSCTYEQSL
AALYKRGLIDRDTALEHAKDIETFKRMVQR

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=466958 H0A61_RS14930 WP_206707879.1 3039686..3040738(-) (pilT) [Koleobacter methoxysyntrophicus strain NRmbB1]
TTGATAACAGACATAATAAAATCCGCACTTGACCAGAAAGCATCGGATATTCATCTTACCGAAGGCCTGCCGCCCGTCTT
CAGAATTAACGGCAGGCTCGCTGTACAGCGTCAGTTTGATGTTTTTTCTGCAGGGGCTCTGGCTGAAACGGCAAAACAGC
TTCTAGGGGAGAATTACGAAAATTACGGTCAAAAACGGAGTTTTGATTTTTCCTTTCATGCCGAAGGCCGGAGGTTCAGG
GGGCATGTCTTTAAACAGAGGGGTGCAGATGCCGTCGTTCTCCGCGTGATACCCGCGGAGGTCCCGACAGTCGATCAGCT
TCTGCTTCCCGAATCCGTAAAAAAATTCACGTCTTTAAGAGACGGGTTGGTGCTGGTTACGGGCCCGACGGGCAGCGGCA
AATCAACGACACTTGCTGCATTAATAAACGAAATTAACAGGGCGCAGGAAAAACATATCATCACCATCGAAGACCCTGTA
GAGTTTATACACCCGCACAGGCGCTGTATAGTCAACCAGCGCGAGGTGGGGACCGATGTAGAAAATTTTGCCGATGCGGT
AAGAAGCGCTATGCGTGAAGACCCGGATATCCTGCTTGTCGGCGAGATGCGCGACCTGGAAACGATTCAGAACGCCATAA
CAATGGCCGAAACGGGCCATCTGGTGTTTGCAACCCTGCATACTCGAAGTGCTGCAGAAACTATAGACAGAATTATAGAC
GTTTTCCCGCCGGTCCAGCAGCAGCAGATACGGGTCCAGCTGTCCAGCATACTGCAGGGGATAATAAGCCAGTGTCTTGT
ACCTGCAGTATCCGGTGGACGTGTGCCCGTCTGTGAAGTAATGTTTGCAACCGACGGGATTAAAAGTATTATAAAGGAAA
ACGGTCCGCACAGTTCTATTAAGGATCAGATTTTGCTTAATCACAGAAAACTCGGTTCGTGTACTTATGAACAAAGCCTG
GCGGCCCTTTACAAAAGAGGATTGATTGACCGCGATACTGCTCTGGAACACGCAAAGGATATAGAGACATTTAAACGTAT
GGTTCAGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

47.324

100

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.724

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.634

100

0.463
  pilT Acinetobacter baumannii D1279779

45.609

100

0.46
  pilT Acinetobacter baumannii strain A118

45.609

100

0.46
  pilT Acinetobacter nosocomialis M2

45.609

100

0.46
  pilT Acinetobacter baylyi ADP1

45.326

100

0.457
  pilT Legionella pneumophila strain ERS1305867

42.34

100

0.434
  pilT Legionella pneumophila strain Lp02

42.34

100

0.434
  pilT Vibrio cholerae strain A1552

45.045

95.143

0.429
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.045

95.143

0.429
  pilT Neisseria meningitidis 8013

42.241

99.429

0.42
  pilT Neisseria gonorrhoeae MS11

41.954

99.429

0.417
  pilU Pseudomonas stutzeri DSM 10701

38.109

99.714

0.38
  pilU Vibrio cholerae strain A1552

35.876

100

0.363