Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H0O22_RS10360 Genome accession   NZ_CP059060
Coordinates   1863663..1864724 (-) Length   353 a.a.
NCBI ID   WP_185186579.1    Uniprot ID   A0A7G7KMW4
Organism   Synechococcus sp. LTW-R     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1858663..1869724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0O22_RS10340 (H0O22_10340) - 1859611..1860162 (+) 552 WP_185186575.1 isoprenylcysteine carboxylmethyltransferase family protein -
  H0O22_RS10345 (H0O22_10345) dusA 1860164..1861162 (-) 999 WP_185186576.1 tRNA dihydrouridine(20/20a) synthase DusA -
  H0O22_RS10350 (H0O22_10350) - 1861188..1862435 (+) 1248 WP_185186577.1 NAD(P)/FAD-dependent oxidoreductase -
  H0O22_RS10355 (H0O22_10355) - 1862428..1863663 (-) 1236 WP_185186578.1 type II secretion system F family protein -
  H0O22_RS10360 (H0O22_10360) pilT 1863663..1864724 (-) 1062 WP_185186579.1 type IV pilus twitching motility protein PilT Machinery gene
  H0O22_RS10365 (H0O22_10365) - 1864791..1866626 (-) 1836 Protein_2042 GspE/PulE family protein -
  H0O22_RS10370 (H0O22_10370) grpE 1866722..1867396 (+) 675 WP_185186580.1 nucleotide exchange factor GrpE -
  H0O22_RS10375 (H0O22_10375) dnaJ 1867399..1868526 (+) 1128 WP_185186581.1 molecular chaperone DnaJ -
  H0O22_RS10380 (H0O22_10380) - 1868534..1868755 (+) 222 WP_185186582.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38874.32 Da        Isoelectric Point: 6.2563

>NTDB_id=466891 H0O22_RS10360 WP_185186579.1 1863663..1864724(-) (pilT) [Synechococcus sp. LTW-R]
MELQIEQLMEELVEAGGSDLHLAAGQPPYGRFSGALKPMREERLSEDLCNRLIFSMINNAQRKQLEQSWELDCAYGLKGV
SRFRVNVYRQRGSYAACLRALGSTIPSLESLGLPPIVEETSRRPRGLVLVTGPTGSGKTTTLAALLDHINRSRAEHILTI
EDPIEFTYRNEKSVIHQRQLGDDTRSFAAALREDPDVILVGEMRDLETIQLAITASETGHLVFGTLHTSSAAQTVDRMVD
VFPPEQQTQIRVQLSGSLVGVFSQTLCKRQNPKPGQFGRVMAQEILINTPAIANLIREGKTAQLYSQIQTGGQQGMQTLE
RALANLVQAGAVDRSEALAKASKPDELMRLLEG

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=466891 H0O22_RS10360 WP_185186579.1 1863663..1864724(-) (pilT) [Synechococcus sp. LTW-R]
ATGGAGCTCCAGATCGAACAGTTGATGGAGGAACTGGTCGAGGCCGGTGGCAGTGATCTGCACCTGGCCGCGGGCCAGCC
GCCCTACGGGCGCTTCAGCGGCGCGCTCAAACCGATGCGCGAGGAGCGCCTCAGCGAGGACCTCTGCAACCGCTTGATCT
TCTCGATGATCAACAACGCCCAGCGCAAGCAGCTGGAGCAAAGCTGGGAGCTGGACTGCGCCTATGGCCTCAAAGGCGTC
TCGCGCTTCCGGGTGAATGTCTACCGCCAACGGGGCAGCTACGCCGCCTGCCTGCGGGCCCTGGGCAGCACGATTCCGAG
CCTGGAGAGCCTGGGGCTACCCCCGATCGTCGAGGAAACCAGCCGACGGCCCAGGGGGCTGGTGCTGGTGACCGGACCGA
CGGGTTCCGGCAAAACCACCACCCTGGCGGCCCTGCTGGATCACATCAACCGCAGCCGCGCCGAGCACATCCTCACGATC
GAAGACCCGATCGAATTCACCTACCGCAACGAAAAAAGCGTCATCCACCAGCGCCAGCTCGGGGATGACACCCGCAGCTT
TGCCGCGGCCCTCCGGGAGGACCCGGACGTGATCCTCGTCGGCGAAATGCGGGATCTGGAGACGATTCAGCTGGCGATCA
CCGCCTCAGAGACCGGGCATCTGGTCTTCGGCACCCTCCACACCAGCTCAGCCGCGCAAACCGTGGATCGCATGGTCGAC
GTCTTCCCACCCGAGCAGCAAACCCAAATCCGCGTGCAGCTGAGTGGCTCCCTGGTGGGGGTGTTTTCCCAGACCCTCTG
CAAACGCCAGAACCCCAAACCCGGGCAATTCGGGCGGGTGATGGCCCAGGAAATCTTGATCAACACCCCGGCCATCGCCA
ACTTGATCCGGGAGGGCAAAACCGCGCAGCTCTATTCCCAGATCCAAACCGGCGGCCAGCAGGGCATGCAAACCCTGGAG
CGCGCCCTCGCCAACCTGGTGCAGGCCGGCGCCGTGGACCGCAGTGAAGCCCTCGCCAAGGCGAGCAAGCCCGACGAGCT
GATGCGGCTCCTGGAGGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G7KMW4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.716

98.867

0.501
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.432

99.717

0.493
  pilT Acinetobacter baylyi ADP1

49.57

98.867

0.49
  pilT Pseudomonas stutzeri DSM 10701

49.57

98.867

0.49
  pilT Acinetobacter baumannii D1279779

49.143

99.15

0.487
  pilT Acinetobacter nosocomialis M2

49.143

99.15

0.487
  pilT Acinetobacter baumannii strain A118

49.143

99.15

0.487
  pilT Vibrio cholerae strain A1552

50

96.884

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

96.884

0.484
  pilT Legionella pneumophila strain ERS1305867

50.147

96.034

0.482
  pilT Legionella pneumophila strain Lp02

50.147

96.034

0.482
  pilT Neisseria gonorrhoeae MS11

49.401

94.618

0.467
  pilT Neisseria meningitidis 8013

49.401

94.618

0.467
  pilU Pseudomonas stutzeri DSM 10701

37.536

98.867

0.371
  pilU Vibrio cholerae strain A1552

36.723

100

0.368