Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H0H12_RS01535 Genome accession   NZ_CP059052
Coordinates   329305..330303 (+) Length   332 a.a.
NCBI ID   WP_180689128.1    Uniprot ID   A0A7D6A2X5
Organism   Pseudomonas putida strain BS3701     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324305..335303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0H12_RS01505 (H0H12_01505) - 324771..325205 (-) 435 WP_064613754.1 DUF4426 domain-containing protein -
  H0H12_RS01510 (H0H12_01510) metW 325228..325848 (-) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  H0H12_RS01515 (H0H12_01515) - 325856..326995 (-) 1140 WP_003249108.1 homoserine O-acetyltransferase -
  H0H12_RS01520 (H0H12_01520) - 327095..327685 (-) 591 WP_012053960.1 YggT family protein -
  H0H12_RS01525 (H0H12_01525) proC 327696..328514 (-) 819 WP_180689127.1 pyrroline-5-carboxylate reductase -
  H0H12_RS01530 (H0H12_01530) - 328553..329239 (-) 687 WP_004575484.1 YggS family pyridoxal phosphate-dependent enzyme -
  H0H12_RS01535 (H0H12_01535) pilT 329305..330303 (+) 999 WP_180689128.1 type IV pilus twitching motility protein PilT Machinery gene
  H0H12_RS01540 (H0H12_01540) - 330413..331039 (-) 627 WP_180689129.1 C40 family peptidase -
  H0H12_RS01545 (H0H12_01545) - 331206..331625 (+) 420 WP_010955634.1 TM2 domain-containing protein -
  H0H12_RS01550 (H0H12_01550) - 331719..332417 (-) 699 WP_014859104.1 SPOR domain-containing protein -
  H0H12_RS01555 (H0H12_01555) argS 332419..334155 (-) 1737 WP_003249124.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36034.70 Da        Isoelectric Point: 7.0705

>NTDB_id=466845 H0H12_RS01535 WP_180689128.1 329305..330303(+) (pilT) [Pseudomonas putida strain BS3701]
MDVTELLARAVDAGASDLHLATGQIPMLRLDGELQRMGLPPLSQGALIEGMASVLDDDQRRQWAQGDEMDLALELPALGR
FRLNLFRQLNGPAVTFRLIPGRIATVSELDLADVFQAVAQCRDGLILVGGPTGSGKSSTLATLLDQLNRERALHIITLED
PVEVIHASQRSLVNQREMGRHSRGFAQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHARSATSSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATPAVRNLVREGRLAQLFSVMQGGAAEGMRTMEGAL
RRLREKGLIGDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=466845 H0H12_RS01535 WP_180689128.1 329305..330303(+) (pilT) [Pseudomonas putida strain BS3701]
ATGGATGTGACCGAGCTGTTGGCCCGAGCTGTGGATGCGGGGGCTTCAGACCTGCACCTGGCGACGGGCCAGATTCCGAT
GCTGCGCCTGGATGGCGAGTTGCAGCGTATGGGCTTGCCGCCCTTGAGCCAGGGTGCCTTGATCGAGGGCATGGCGAGCG
TGCTGGATGACGATCAGCGCCGGCAGTGGGCCCAAGGCGATGAAATGGACCTTGCGCTGGAGTTGCCGGCGCTTGGGCGC
TTTCGGCTGAACCTGTTTCGCCAGTTGAATGGCCCGGCGGTTACCTTTCGGTTGATTCCGGGGCGCATTGCGACGGTGAG
TGAGCTGGACCTTGCGGATGTGTTTCAAGCTGTTGCGCAATGCCGGGATGGCTTGATCCTCGTGGGCGGGCCGACCGGCA
GTGGCAAGTCCAGTACCCTGGCAACGCTGCTCGACCAGCTGAACCGCGAGCGGGCGCTGCACATCATTACCCTGGAAGAC
CCTGTCGAGGTCATCCATGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGATGGGGCGCCATAGTCGCGGCTTTGCTCA
GGGGCTGCGCAGTGCGCTGCGCCAGGACCCGGACGTGATCATGATCGGCGAGTTGCGCGACCTGGAAACCATTCGTCTGG
CCCTGCGCGCGGCCGAAACCGGGCACCTGGTGCTGGCAACCGTGCATGCGCGTTCGGCGACCAGCAGCATCGACAGGTTG
GTGGAGGTTTTTGCTGCCGAGGAAAAGCCGTTGGTGCGGGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTCGCGCAGGT
GCTGGTCAGGCGCGTGGGGGGCGGGCGGGTGGCGGCGCGGGAAGTGCTGGTGGCGACGCCGGCGGTGCGTAACCTGGTTC
GGGAGGGGCGCTTGGCGCAGTTGTTTTCGGTGATGCAGGGCGGGGCGGCGGAGGGGATGCGGACGATGGAGGGGGCGTTG
CGGAGGCTGAGGGAGAAGGGTTTGATCGGTGATCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7D6A2X5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.672

99.096

0.512
  pilT Pseudomonas stutzeri DSM 10701

50.76

99.096

0.503
  pilT Acinetobacter baylyi ADP1

50.606

99.398

0.503
  pilT Acinetobacter nosocomialis M2

50.303

99.398

0.5
  pilT Acinetobacter baumannii D1279779

50.303

99.398

0.5
  pilT Acinetobacter baumannii strain A118

50.303

99.398

0.5
  pilT Legionella pneumophila strain Lp02

49.394

99.398

0.491
  pilT Legionella pneumophila strain ERS1305867

49.394

99.398

0.491
  pilT Neisseria meningitidis 8013

48.943

99.699

0.488
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Neisseria gonorrhoeae MS11

48.64

99.699

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.758

99.398

0.455
  pilU Pseudomonas stutzeri DSM 10701

37.879

99.398

0.377
  pilU Vibrio cholerae strain A1552

37.037

97.59

0.361