Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV033_RS18940 Genome accession   NZ_CP058191
Coordinates   3962397..3963377 (-) Length   326 a.a.
NCBI ID   WP_063146832.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain RHBSTW-00012     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3957397..3968377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV033_RS18910 (HV033_18910) - 3958274..3958771 (+) 498 WP_023298567.1 SprT family zinc-dependent metalloprotease -
  HV033_RS18915 (HV033_18915) endA 3958866..3959573 (+) 708 WP_022649352.1 deoxyribonuclease I -
  HV033_RS18920 (HV033_18920) rsmE 3959625..3960356 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV033_RS18925 (HV033_18925) gshB 3960388..3961335 (+) 948 WP_003860032.1 glutathione synthase -
  HV033_RS18930 (HV033_18930) - 3961410..3961970 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  HV033_RS18935 (HV033_18935) ruvX 3961970..3962386 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  HV033_RS18940 (HV033_18940) pilT 3962397..3963377 (-) 981 WP_063146832.1 type IV pilus twitching motility protein PilT Machinery gene
  HV033_RS18945 (HV033_18945) - 3963395..3964096 (+) 702 WP_023298569.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV033_RS18950 (HV033_18950) - 3964118..3964684 (+) 567 WP_003860024.1 YggT family protein -
  HV033_RS18955 (HV033_18955) yggU 3964681..3964977 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  HV033_RS18960 (HV033_18960) - 3964981..3965574 (+) 594 WP_063146831.1 XTP/dITP diphosphatase -
  HV033_RS18965 (HV033_18965) hemW 3965567..3966715 (+) 1149 WP_063929308.1 radical SAM family heme chaperone HemW -
  HV033_RS18970 (HV033_18970) - 3966772..3967110 (+) 339 WP_063146829.1 DUF559 domain-containing protein -
  HV033_RS18975 (HV033_18975) - 3967202..3967918 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  HV033_RS18980 (HV033_18980) - 3967976..3968302 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35631.61 Da        Isoelectric Point: 6.3407

>NTDB_id=462569 HV033_RS18940 WP_063146832.1 3962397..3963377(-) (pilT) [Enterobacter hormaechei strain RHBSTW-00012]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
LAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=462569 HV033_RS18940 WP_063146832.1 3962397..3963377(-) (pilT) [Enterobacter hormaechei strain RHBSTW-00012]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGTGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTTCGCGACAGCGAGACGATACGCCTGGCGTTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGACTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGTA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CTGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.69

100

0.38