Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HVZ39_RS04665 Genome accession   NZ_CP057147
Coordinates   942332..943312 (+) Length   326 a.a.
NCBI ID   WP_181633272.1    Uniprot ID   -
Organism   Citrobacter sp. RHB35-C21     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937332..948312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HVZ39_RS04625 (HVZ39_04630) hemW 937753..938889 (-) 1137 WP_047357839.1 radical SAM family heme chaperone HemW -
  HVZ39_RS04630 (HVZ39_04635) - 938882..939475 (-) 594 WP_181633269.1 XTP/dITP diphosphatase -
  HVZ39_RS04635 (HVZ39_04640) yggU 939483..939773 (-) 291 WP_046274950.1 DUF167 family protein YggU -
  HVZ39_RS04640 (HVZ39_04645) - 939770..940336 (-) 567 WP_016154401.1 YggT family protein -
  HVZ39_RS04645 (HVZ39_04650) - 940355..941059 (-) 705 WP_181633270.1 YggS family pyridoxal phosphate-dependent enzyme -
  HVZ39_RS04650 (HVZ39_04655) - 941032..941196 (+) 165 Protein_921 type IV pili twitching motility protein PilT -
  HVZ39_RS04655 (HVZ39_04660) - 941719..942138 (+) 420 WP_181633271.1 DUF3828 domain-containing protein -
  HVZ39_RS04660 (HVZ39_04665) - 942144..942314 (-) 171 Protein_923 YggS family pyridoxal phosphate enzyme -
  HVZ39_RS04665 (HVZ39_04670) pilT 942332..943312 (+) 981 WP_181633272.1 type IV pilus twitching motility protein PilT Machinery gene
  HVZ39_RS04670 (HVZ39_04675) ruvX 943309..943725 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  HVZ39_RS04675 (HVZ39_04680) - 943725..944288 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  HVZ39_RS04680 (HVZ39_04685) gshB 944465..945412 (-) 948 WP_016154394.1 glutathione synthase -
  HVZ39_RS04685 (HVZ39_04690) rsmE 945425..946156 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HVZ39_RS04690 (HVZ39_04695) endA 946232..946939 (-) 708 WP_101700211.1 deoxyribonuclease I -
  HVZ39_RS04695 (HVZ39_04700) - 947034..947531 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36016.08 Da        Isoelectric Point: 6.3395

>NTDB_id=461354 HVZ39_RS04665 WP_181633272.1 942332..943312(+) (pilT) [Citrobacter sp. RHB35-C21]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVETAPFTTPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDELQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=461354 HVZ39_RS04665 WP_181633272.1 942332..943312(+) (pilT) [Citrobacter sp. RHB35-C21]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAACCGCACCATTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGAGCTCCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGTTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387