Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HWQ17_RS17310 Genome accession   NZ_CP056779
Coordinates   3618352..3619332 (+) Length   326 a.a.
NCBI ID   WP_148418515.1    Uniprot ID   -
Organism   Enterobacter pasteurii strain D41-sc-1712200     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3613352..3624332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ17_RS17270 (HWQ17_17270) - 3613437..3613763 (+) 327 WP_003862421.1 YggL family protein -
  HWQ17_RS17275 (HWQ17_17275) - 3613820..3614536 (+) 717 WP_148418509.1 DUF2884 domain-containing protein -
  HWQ17_RS17280 (HWQ17_17280) - 3614619..3614957 (-) 339 WP_148418510.1 endonuclease domain-containing protein -
  HWQ17_RS17285 (HWQ17_17285) hemW 3615014..3616162 (-) 1149 WP_148418511.1 radical SAM family heme chaperone HemW -
  HWQ17_RS17290 (HWQ17_17290) - 3616155..3616748 (-) 594 WP_148418512.1 XTP/dITP diphosphatase -
  HWQ17_RS17295 (HWQ17_17295) yggU 3616752..3617048 (-) 297 WP_047364545.1 DUF167 family protein YggU -
  HWQ17_RS17300 (HWQ17_17300) - 3617045..3617611 (-) 567 WP_148418513.1 YggT family protein -
  HWQ17_RS17305 (HWQ17_17305) - 3617633..3618334 (-) 702 WP_148418514.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWQ17_RS17310 (HWQ17_17310) pilT 3618352..3619332 (+) 981 WP_148418515.1 type IV pilus twitching motility protein PilT Machinery gene
  HWQ17_RS17315 (HWQ17_17315) ruvX 3619343..3619759 (-) 417 WP_114979901.1 Holliday junction resolvase RuvX -
  HWQ17_RS17320 (HWQ17_17320) - 3619759..3620319 (-) 561 WP_148418516.1 YqgE/AlgH family protein -
  HWQ17_RS17325 (HWQ17_17325) gshB 3620394..3621341 (-) 948 WP_148418517.1 glutathione synthase -
  HWQ17_RS17330 (HWQ17_17330) rsmE 3621361..3622092 (-) 732 WP_148418518.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HWQ17_RS17335 (HWQ17_17335) endA 3622144..3622851 (-) 708 WP_148418519.1 deoxyribonuclease I -
  HWQ17_RS17340 (HWQ17_17340) - 3622946..3623443 (-) 498 WP_061097110.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35671.68 Da        Isoelectric Point: 6.4114

>NTDB_id=460606 HWQ17_RS17310 WP_148418515.1 3618352..3619332(+) (pilT) [Enterobacter pasteurii strain D41-sc-1712200]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPPPDVEALLKAWLNDEQQGAWWASGQVDFAVTVAGNQRLRG
SAFRHMQGVSIALRLLPLACPQLSTLGVPRTIPALLCNDDGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FRYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDI
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=460606 HWQ17_RS17310 WP_148418515.1 3618352..3619332(+) (pilT) [Enterobacter pasteurii strain D41-sc-1712200]
ATGGATGTGGAAGAAATTGTTGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCGCCGCG
CTGGCGCAGAGCGGGGCGCCTTGAACCCGCGCCGTTTCCGCCCCCGGACGTGGAGGCGTTATTGAAAGCGTGGCTCAACG
ACGAGCAGCAGGGCGCATGGTGGGCGAGCGGGCAGGTGGATTTTGCCGTTACCGTTGCGGGCAATCAGCGTCTGCGCGGC
AGCGCGTTCAGGCATATGCAGGGCGTTTCGATCGCGCTGCGGCTGTTGCCGCTGGCGTGCCCGCAGCTCTCCACGTTAGG
CGTGCCGCGAACGATCCCGGCGCTACTGTGCAATGACGATGGTCTGATTCTGGTCACCGGCGCAACCGGCAGCGGGAAGT
CAACGACGCTTGCAGCGATGGTCGATTTTCTCAATCACCATACGGACGGCCATATTCTTACCCTGGAAGATCCGGTGGAG
TTCAGGTACCAGAGCGAACGCTGTTTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCTGATGTCATTTTGCTTGGCGAGCTGCGCGACAGCGAAACTATCCGCCTGGCGCTGACGG
CGGCTGAGACCGGGCATCTGGTGTTAGCCACGCTGCATACGCGCGGTGCGGCGCAGGCAATTGAGCGCCTGGTGGATATC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTGCTGGCTCAGAAGCTGCGTCA
GGACGTTCAGGGCGGGCGCGTGGCGCTGTATGAGCTTCTGGTGAATACCCCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACGTGGCAACTGCCGGGCATTATTCAAACCGGACAGCAGGCGGGTATGCAGAACTTTGAGCAGAGCCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.724

99.08

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383