Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HV254_RS03865 Genome accession   NZ_CP056676
Coordinates   806908..807888 (+) Length   326 a.a.
NCBI ID   WP_045623032.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain RHBSTW-00642     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801908..812888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV254_RS03825 (HV254_03825) - 801959..802285 (+) 327 WP_003862421.1 YggL family protein -
  HV254_RS03830 (HV254_03830) - 802343..803059 (+) 717 WP_022649360.1 DUF2884 domain-containing protein -
  HV254_RS03835 (HV254_03835) - 803175..803513 (-) 339 WP_023298571.1 endonuclease domain-containing protein -
  HV254_RS03840 (HV254_03840) hemW 803570..804718 (-) 1149 WP_023306915.1 radical SAM family heme chaperone HemW -
  HV254_RS03845 (HV254_03845) - 804711..805304 (-) 594 WP_022649357.1 XTP/dITP diphosphatase -
  HV254_RS03850 (HV254_03850) yggU 805308..805604 (-) 297 WP_022649356.1 DUF167 family protein YggU -
  HV254_RS03855 (HV254_03855) - 805601..806167 (-) 567 WP_003860024.1 YggT family protein -
  HV254_RS03860 (HV254_03860) - 806189..806890 (-) 702 WP_045623033.1 YggS family pyridoxal phosphate-dependent enzyme -
  HV254_RS03865 (HV254_03865) pilT 806908..807888 (+) 981 WP_045623032.1 type IV pilus twitching motility protein PilT Machinery gene
  HV254_RS03870 (HV254_03870) ruvX 807899..808315 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  HV254_RS03875 (HV254_03875) - 808315..808875 (-) 561 WP_045623031.1 YqgE/AlgH family protein -
  HV254_RS03880 (HV254_03880) gshB 808950..809897 (-) 948 WP_003860032.1 glutathione synthase -
  HV254_RS03885 (HV254_03885) rsmE 809929..810660 (-) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HV254_RS03890 (HV254_03890) endA 810712..811419 (-) 708 WP_045623030.1 deoxyribonuclease I -
  HV254_RS03895 (HV254_03895) - 811514..812011 (-) 498 WP_023298567.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35746.71 Da        Isoelectric Point: 6.3417

>NTDB_id=460142 HV254_RS03865 WP_045623032.1 806908..807888(+) (pilT) [Enterobacter hormaechei strain RHBSTW-00642]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQERL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=460142 HV254_RS03865 WP_045623032.1 806908..807888(+) (pilT) [Enterobacter hormaechei strain RHBSTW-00642]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGCGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGTTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACTGGCGCTACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGTA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGAGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.411

97.853

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.411

97.853

0.503
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.975

98.466

0.472
  pilT Acinetobacter baylyi ADP1

46.933

100

0.469
  pilT Neisseria gonorrhoeae MS11

47.664

98.466

0.469
  pilT Acinetobacter baumannii strain A118

47.5

98.16

0.466
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Acinetobacter baumannii D1279779

47.5

98.16

0.466
  pilT Pseudomonas aeruginosa PAK

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.226

100

0.383
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38