Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HWV00_RS19035 Genome accession   NZ_CP056123
Coordinates   4269610..4270725 (-) Length   371 a.a.
NCBI ID   WP_211683798.1    Uniprot ID   -
Organism   Moritella sp. 24     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4264610..4275725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV00_RS19015 (HWV00_19015) - 4265857..4266537 (+) 681 WP_211683794.1 TIGR00153 family protein -
  HWV00_RS19020 (HWV00_19020) - 4266560..4267834 (+) 1275 WP_211683795.1 inorganic phosphate transporter -
  HWV00_RS19025 (HWV00_19025) - 4267994..4268641 (+) 648 WP_211683796.1 TIGR04211 family SH3 domain-containing protein -
  HWV00_RS19030 (HWV00_19030) yaaA 4268750..4269529 (-) 780 WP_211683797.1 peroxide stress protein YaaA -
  HWV00_RS19035 (HWV00_19035) pilU 4269610..4270725 (-) 1116 WP_211683798.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWV00_RS19040 (HWV00_19040) pilT 4270737..4271771 (-) 1035 WP_211683799.1 type IV pilus twitching motility protein PilT Machinery gene
  HWV00_RS19045 (HWV00_19045) - 4271802..4272497 (+) 696 WP_211683800.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWV00_RS19050 (HWV00_19050) proC 4272556..4273383 (+) 828 WP_211683801.1 pyrroline-5-carboxylate reductase -
  HWV00_RS19055 (HWV00_19055) - 4273441..4273986 (+) 546 WP_211683802.1 YggT family protein -
  HWV00_RS19060 (HWV00_19060) - 4274100..4274528 (+) 429 WP_211683803.1 DUF4426 domain-containing protein -
  HWV00_RS19065 (HWV00_19065) - 4274631..4275245 (+) 615 WP_211683804.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41159.29 Da        Isoelectric Point: 5.8704

>NTDB_id=458295 HWV00_RS19035 WP_211683798.1 4269610..4270725(-) (pilU) [Moritella sp. 24]
MGMLPQLFSAMKKLEGSDMYISAGIAPTVKVHGSLRALSEHKLTAEQSLSLVKEAMSEDEYASFCRTKESNFGIFMPEIG
RFRVSAFWQLEKAGMVIRRIETDIPAMDELYLPQILKHTALEKRGLVLVVGATGSGKSTTQAAMVGYRNQNSSGHILTIE
DPIEFVHEHDSCIVTQREVGIDTESFEDALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHATNANQAIDR
ILHLVPKEKHAQLLYDLSLNLKAIIAQQLIPVQGGTSRRGAFEILLNTPLVSEHIRKNELHKIKELMSKSIEQGMQTFDQ
ALLELYNTNMISYSEALHHADSPNDLRLMIKLNQGQAASDSMLDGVTIEAL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=458295 HWV00_RS19035 WP_211683798.1 4269610..4270725(-) (pilU) [Moritella sp. 24]
ATGGGGATGTTACCGCAACTCTTTAGTGCAATGAAAAAGCTCGAGGGTTCTGATATGTATATTTCTGCTGGTATTGCGCC
AACAGTGAAGGTACATGGTTCGCTACGTGCGCTTTCAGAGCATAAGTTAACGGCAGAGCAATCATTATCATTAGTGAAAG
AAGCAATGTCAGAAGATGAGTATGCTTCTTTTTGCCGCACTAAGGAGTCTAACTTTGGTATTTTTATGCCAGAGATTGGT
CGTTTCCGTGTGAGTGCATTTTGGCAATTAGAAAAAGCTGGCATGGTTATTCGTCGTATTGAAACGGACATACCAGCGAT
GGATGAATTATACCTGCCACAAATATTAAAACACACTGCATTAGAAAAACGCGGTTTGGTATTGGTTGTTGGTGCTACAG
GCTCGGGTAAGTCAACGACGCAAGCTGCAATGGTTGGTTATCGAAATCAAAATTCATCGGGGCATATTTTAACGATTGAA
GATCCGATTGAGTTTGTGCATGAACATGATAGTTGTATTGTGACACAGCGTGAAGTGGGTATTGATACCGAATCATTTGA
AGATGCGCTAAAGAGCTCATTGCGTCAAGCGCCGGATGTCATTCTTATTGGTGAGATCCGTTCGCAAGAAACAATGGAGT
TTGCGCTCGCATTTGCTGAAACGGGTCATTTATGTATGGCGACACTGCACGCAACCAACGCGAATCAGGCTATCGACCGT
ATTTTACATTTGGTTCCAAAAGAAAAACACGCGCAGTTATTATATGATTTGTCGCTAAACTTAAAAGCCATTATTGCCCA
GCAGTTAATACCTGTGCAAGGTGGTACAAGTCGACGTGGTGCATTTGAAATTTTACTCAATACCCCGTTGGTGAGCGAGC
ATATTCGTAAGAATGAATTGCATAAGATTAAAGAGCTGATGTCTAAATCGATAGAGCAGGGCATGCAGACTTTTGATCAA
GCATTATTAGAACTTTATAACACCAACATGATTAGTTACAGTGAAGCGTTGCACCATGCGGATTCACCGAATGATTTACG
CTTAATGATTAAGTTAAATCAAGGGCAAGCTGCGAGTGATAGTATGCTCGACGGTGTGACGATTGAAGCGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.016

98.652

0.582
  pilU Pseudomonas stutzeri DSM 10701

59.078

93.531

0.553
  pilU Acinetobacter baylyi ADP1

58.166

94.07

0.547
  pilT Pseudomonas aeruginosa PAK

41.888

91.375

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.727

88.949

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.413

91.375

0.369
  pilT Acinetobacter nosocomialis M2

40.294

91.644

0.369
  pilT Acinetobacter baumannii D1279779

40.294

91.644

0.369
  pilT Acinetobacter baumannii strain A118

40.294

91.644

0.369
  pilT Vibrio cholerae strain A1552

42.105

87.062

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.105

87.062

0.367
  pilT Legionella pneumophila strain Lp02

41.033

88.679

0.364
  pilT Legionella pneumophila strain ERS1305867

41.033

88.679

0.364
  pilT Acinetobacter baylyi ADP1

39.881

90.566

0.361