Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HWV01_RS01750 Genome accession   NZ_CP056122
Coordinates   370537..371652 (+) Length   371 a.a.
NCBI ID   WP_304610881.1    Uniprot ID   -
Organism   Moritella sp. 5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 365537..376652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV01_RS01720 (HWV01_01715) - 366027..366641 (-) 615 WP_211673796.1 XTP/dITP diphosphatase -
  HWV01_RS01725 (HWV01_01720) - 366749..367177 (-) 429 WP_211673797.1 DUF4426 domain-containing protein -
  HWV01_RS01730 (HWV01_01725) - 367294..367839 (-) 546 WP_211673798.1 YggT family protein -
  HWV01_RS01735 (HWV01_01730) proC 367881..368708 (-) 828 WP_211673799.1 pyrroline-5-carboxylate reductase -
  HWV01_RS01740 (HWV01_01735) - 368765..369460 (-) 696 WP_211673800.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWV01_RS01745 (HWV01_01740) pilT 369491..370525 (+) 1035 WP_067045686.1 type IV pilus twitching motility protein PilT Machinery gene
  HWV01_RS01750 (HWV01_01745) pilU 370537..371652 (+) 1116 WP_304610881.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWV01_RS01755 (HWV01_01750) yaaA 371716..372495 (+) 780 WP_211673801.1 peroxide stress protein YaaA -
  HWV01_RS01760 (HWV01_01755) - 372651..373298 (-) 648 WP_211673802.1 TIGR04211 family SH3 domain-containing protein -
  HWV01_RS01765 (HWV01_01760) - 373457..374731 (-) 1275 WP_211673803.1 inorganic phosphate transporter -
  HWV01_RS01770 (HWV01_01765) - 374755..375435 (-) 681 WP_211673804.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40805.02 Da        Isoelectric Point: 6.2693

>NTDB_id=458261 HWV01_RS01750 WP_304610881.1 370537..371652(+) (pilU) [Moritella sp. 5]
MGMLPQLFSAMKKLTGSDMYISAGIAPTVKVHGSLRALSEHKLTAEQSLALVKEAMSEDEYASFCRTKESNFGIFMPDIG
RFRVSAFWQLEKAGMVIRRIETDIPAMDELHLPPILKHTALEKRGLVLVVGATGSGKSTTQAAMVGYRNQNSSGHILTIE
DPIEFVHEHGSCIVTQREVGIDTESFEDALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHATNANQAIDR
ILHLVPKEKHAQLLYDLSLNLKAIVAQQLIPVQGGSSRRGAFEILLNTPLVSELIRKNELHKIKEVMTKSGELGMQTFDQ
ALLALYNTNMISYTEALHHADSPNDLRLMIKLNQGQAGSDSMLDGVTIEAL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=458261 HWV01_RS01750 WP_304610881.1 370537..371652(+) (pilU) [Moritella sp. 5]
ATGGGGATGCTACCACAACTCTTTAGTGCGATGAAAAAGCTTACTGGTTCTGACATGTATATTTCGGCGGGTATTGCACC
GACAGTGAAAGTACATGGCTCATTACGTGCGCTCTCGGAACATAAATTAACGGCTGAGCAATCATTAGCATTGGTGAAAG
AAGCCATGTCTGAGGATGAATATGCTTCTTTTTGCCGTACCAAAGAATCTAATTTCGGTATTTTCATGCCGGATATTGGT
CGTTTCCGTGTCAGCGCATTTTGGCAATTAGAAAAAGCGGGTATGGTGATCCGTCGTATTGAAACCGATATACCAGCAAT
GGATGAGCTACACTTGCCACCAATATTAAAACACACAGCGCTAGAGAAGCGTGGTTTAGTGTTGGTTGTTGGGGCGACGG
GTTCTGGTAAATCAACCACGCAAGCAGCCATGGTGGGTTATCGTAACCAAAACTCATCAGGTCATATCTTAACGATTGAA
GATCCGATTGAGTTTGTCCATGAACATGGCAGTTGTATTGTGACTCAGCGTGAAGTCGGGATTGATACCGAATCTTTTGA
AGATGCGCTGAAGAGCTCATTACGTCAAGCGCCGGATGTGATCTTGATTGGTGAAATCCGCTCGCAAGAAACGATGGAGT
TTGCCTTGGCGTTTGCGGAAACGGGGCATTTATGTATGGCAACGCTGCATGCAACTAATGCCAATCAGGCTATCGATCGT
ATTTTACACTTGGTACCAAAAGAAAAGCATGCTCAGTTGTTATATGATTTATCACTCAATTTAAAAGCCATTGTGGCGCA
GCAATTAATCCCAGTACAAGGTGGTTCAAGCCGACGTGGTGCATTTGAAATTTTATTGAATACGCCTTTGGTCAGTGAAT
TGATCCGCAAAAATGAACTGCATAAAATTAAAGAAGTGATGACAAAATCGGGCGAGCTGGGAATGCAAACCTTCGACCAA
GCGTTATTAGCACTTTATAATACCAATATGATTAGTTATACCGAGGCTTTACATCACGCTGACTCGCCAAATGATTTACG
CTTAATGATTAAGTTAAATCAAGGCCAAGCAGGCAGTGATAGTATGCTTGATGGGGTGACGATCGAAGCTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.563

98.652

0.588
  pilU Pseudomonas stutzeri DSM 10701

57.923

98.652

0.571
  pilU Acinetobacter baylyi ADP1

58.453

94.07

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.733

92.722

0.396
  pilT Pseudomonas aeruginosa PAK

42.773

91.375

0.391
  pilT Pseudomonas stutzeri DSM 10701

41.298

91.375

0.377
  pilT Legionella pneumophila strain Lp02

41.641

88.679

0.369
  pilT Legionella pneumophila strain ERS1305867

41.641

88.679

0.369
  pilT Acinetobacter baumannii D1279779

40

91.644

0.367
  pilT Acinetobacter baumannii strain A118

40

91.644

0.367
  pilT Acinetobacter nosocomialis M2

40

91.644

0.367
  pilT Acinetobacter baylyi ADP1

39.881

90.566

0.361
  pilT Neisseria gonorrhoeae MS11

40

90.296

0.361
  pilT Vibrio cholerae strain A1552

43.506

83.019

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.506

83.019

0.361