Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HWV03_RS01715 Genome accession   NZ_CP056120
Coordinates   367932..369047 (+) Length   371 a.a.
NCBI ID   WP_274609799.1    Uniprot ID   -
Organism   Moritella sp. 36     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362932..374047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWV03_RS01685 (HWV03_01685) - 363393..364007 (-) 615 WP_211678204.1 XTP/dITP diphosphatase -
  HWV03_RS01690 (HWV03_01690) - 364143..364571 (-) 429 WP_211678205.1 DUF4426 domain-containing protein -
  HWV03_RS01695 (HWV03_01695) - 364689..365234 (-) 546 WP_211678206.1 YggT family protein -
  HWV03_RS01700 (HWV03_01700) proC 365276..366103 (-) 828 WP_211678207.1 pyrroline-5-carboxylate reductase -
  HWV03_RS01705 (HWV03_01705) - 366160..366855 (-) 696 WP_211678208.1 YggS family pyridoxal phosphate-dependent enzyme -
  HWV03_RS01710 (HWV03_01710) pilT 366886..367920 (+) 1035 WP_211678209.1 type IV pilus twitching motility protein PilT Machinery gene
  HWV03_RS01715 (HWV03_01715) pilU 367932..369047 (+) 1116 WP_274609799.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HWV03_RS01720 (HWV03_01720) yaaA 369111..369890 (+) 780 WP_211678210.1 peroxide stress protein YaaA -
  HWV03_RS01725 (HWV03_01725) - 370046..370693 (-) 648 WP_211678211.1 TIGR04211 family SH3 domain-containing protein -
  HWV03_RS01730 (HWV03_01730) - 370853..372127 (-) 1275 WP_211678212.1 inorganic phosphate transporter -
  HWV03_RS01735 (HWV03_01735) - 372151..372831 (-) 681 WP_211678213.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40865.06 Da        Isoelectric Point: 6.2700

>NTDB_id=458213 HWV03_RS01715 WP_274609799.1 367932..369047(+) (pilU) [Moritella sp. 36]
MGMLPQLFSAMKKLTGSDMYISAGIAPTVKVHGSLRALSEHKLTAEQSLALVKEAMSEDEYASFYRTKESNFGIFMPDIG
RFRVSAFWQLEKAGMVIRRIETDIPAMDELHLPPILKHTALEKRGLVLVVGATGSGKSTTQAAMVGYRNQNSSGHILTIE
DPIEFVHEHGSCIVTQREVGIDTESFEDALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHATNANQAIDR
ILHLVPKEKHAQLLYDLSLNLKAIVAQQLIPVQGGSSRRGAFEILLNTPLVSELIRKNELHKIKEVMTKSGELGMQTFDQ
ALLALYNTNMISYTEALHHADSPNDLRLMIKLNQGQAGSDSMLDGVTIEAL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=458213 HWV03_RS01715 WP_274609799.1 367932..369047(+) (pilU) [Moritella sp. 36]
ATGGGGATGCTACCACAACTCTTTAGTGCGATGAAAAAGCTTACTGGTTCTGACATGTATATTTCGGCGGGTATTGCACC
CACAGTGAAAGTACATGGCTCATTACGTGCGCTCTCGGAACATAAATTAACGGCTGAGCAATCATTAGCATTGGTGAAAG
AAGCCATGTCTGAGGATGAATATGCTTCTTTTTACCGTACCAAAGAATCTAATTTCGGTATTTTCATGCCGGATATTGGT
CGTTTCCGTGTCAGCGCATTTTGGCAATTAGAAAAAGCGGGTATGGTGATCCGTCGTATTGAAACCGATATACCAGCAAT
GGATGAGCTACACTTGCCACCAATATTAAAACACACAGCGCTAGAGAAGCGTGGTTTAGTGTTGGTTGTTGGTGCGACGG
GTTCTGGTAAATCAACCACGCAAGCAGCCATGGTGGGTTATCGTAACCAAAACTCATCAGGTCATATCTTAACGATTGAA
GATCCGATTGAGTTTGTCCATGAACATGGTAGTTGTATTGTTACTCAGCGTGAAGTAGGAATTGATACCGAGTCTTTTGA
AGATGCGCTGAAGAGCTCATTACGTCAAGCGCCAGATGTGATCTTGATTGGTGAAATCCGCTCGCAAGAAACGATGGAGT
TTGCCTTGGCGTTTGCGGAAACTGGGCATTTATGTATGGCAACGCTGCATGCAACTAATGCCAATCAGGCTATCGATCGT
ATTTTGCACTTAGTACCAAAAGAAAAGCATGCTCAGTTGTTATATGATTTGTCACTAAATTTAAAAGCCATTGTGGCGCA
GCAATTAATCCCAGTACAGGGTGGTTCAAGCCGGCGTGGTGCATTTGAAATTTTATTGAATACACCTTTGGTCAGTGAAT
TGATCCGCAAAAATGAACTGCATAAAATTAAAGAAGTGATGACAAAATCGGGCGAGCTGGGAATGCAAACCTTCGATCAA
GCGTTACTAGCACTTTATAATACCAATATGATTAGTTATACCGAGGCTTTACATCATGCTGACTCGCCAAATGATTTACG
CTTAATGATTAAGTTAAATCAAGGCCAAGCAGGCAGTGATAGTATGCTTGACGGGGTGACGATTGAAGCTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.563

98.652

0.588
  pilU Pseudomonas stutzeri DSM 10701

57.923

98.652

0.571
  pilU Acinetobacter baylyi ADP1

58.453

94.07

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.733

92.722

0.396
  pilT Pseudomonas aeruginosa PAK

42.773

91.375

0.391
  pilT Legionella pneumophila strain Lp02

42.169

89.488

0.377
  pilT Legionella pneumophila strain ERS1305867

42.169

89.488

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.298

91.375

0.377
  pilT Acinetobacter baumannii D1279779

40

91.644

0.367
  pilT Acinetobacter baumannii strain A118

40

91.644

0.367
  pilT Acinetobacter nosocomialis M2

40

91.644

0.367
  pilT Acinetobacter baylyi ADP1

39.881

90.566

0.361
  pilT Neisseria gonorrhoeae MS11

40

90.296

0.361
  pilT Vibrio cholerae strain A1552

43.506

83.019

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.506

83.019

0.361