Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUI94_RS16270 Genome accession   NZ_CP055276
Coordinates   3435439..3436419 (+) Length   326 a.a.
NCBI ID   WP_218400343.1    Uniprot ID   -
Organism   Enterobacter mori strain CX01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3430439..3441419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUI94_RS16230 (HUI94_16205) - 3430514..3430840 (+) 327 WP_010435485.1 YggL family protein -
  HUI94_RS16235 (HUI94_16210) - 3430898..3431614 (+) 717 WP_042714747.1 DUF2884 domain-containing protein -
  HUI94_RS16240 (HUI94_16215) - 3431681..3432043 (-) 363 WP_100166387.1 endonuclease domain-containing protein -
  HUI94_RS16245 (HUI94_16220) hemW 3432107..3433249 (-) 1143 WP_218400339.1 radical SAM family heme chaperone HemW -
  HUI94_RS16250 (HUI94_16225) - 3433242..3433835 (-) 594 WP_218400341.1 XTP/dITP diphosphatase -
  HUI94_RS16255 (HUI94_16230) yggU 3433839..3434135 (-) 297 WP_042714751.1 DUF167 family protein YggU -
  HUI94_RS16260 (HUI94_16235) - 3434132..3434698 (-) 567 WP_010435467.1 YggT family protein -
  HUI94_RS16265 (HUI94_16240) - 3434720..3435421 (-) 702 WP_100166385.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUI94_RS16270 (HUI94_16245) pilT 3435439..3436419 (+) 981 WP_218400343.1 type IV pilus twitching motility protein PilT Machinery gene
  HUI94_RS16275 (HUI94_16250) ruvX 3436430..3436846 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  HUI94_RS16280 (HUI94_16255) - 3436846..3437406 (-) 561 WP_010435456.1 YqgE/AlgH family protein -
  HUI94_RS16285 (HUI94_16260) gshB 3437481..3438428 (-) 948 WP_010435454.1 glutathione synthase -
  HUI94_RS16290 (HUI94_16265) rsmE 3438448..3439179 (-) 732 WP_010435451.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HUI94_RS16295 (HUI94_16270) endA 3439231..3439938 (-) 708 WP_218308353.1 deoxyribonuclease I -
  HUI94_RS16300 (HUI94_16275) - 3440033..3440530 (-) 498 WP_010435446.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35690.79 Da        Isoelectric Point: 6.0392

>NTDB_id=457349 HUI94_RS16270 WP_218400343.1 3435439..3436419(+) (pilT) [Enterobacter mori strain CX01]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRG
SAFTHMKGVSIALRLLPLACPQLSALGVPRAIPELLSSESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREMCQHSLSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=457349 HUI94_RS16270 WP_218400343.1 3435439..3436419(+) (pilT) [Enterobacter mori strain CX01]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCGGCCCCGTTTCCGGCCCCGGACGTTGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGACTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCATATGAAGGGGGTGTCGATCGCCTTGCGCCTGTTGCCGCTCGCGTGCCCGCAGCTTTCTGCACTGGG
TGTGCCCAGGGCTATCCCGGAACTGCTCTCCAGCGAAAGCGGGTTGATTCTGGTGACGGGGGCGACCGGTAGCGGAAAAT
CCACGACGCTTGCGGCGATGGTGGATTTTCTCAACCATCACACTGACGGCCACATACTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGCTGCCTGATACAGCAGCGGGAGATGTGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
CAGTGCGCTGCGCGAAGATCCGGACGTTATTCTGTTAGGAGAGCTGCGCGACAGCGAAACCATCCGCCTTGCGCTGACGG
CGGCCGAAACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGCGCTTCGCAGGCGATTGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTTCGCGCTGTACTGGCGCAAAAACTGCTCCA
GGACGTGCAGGGCGGGCGCGTGGCGCTTTATGAACTGCTGGTGAACACCCCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AAACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTCGCGGAAAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377