Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   G9X25_RS03595 Genome accession   NZ_CP054827
Coordinates   750681..751661 (+) Length   326 a.a.
NCBI ID   WP_175268140.1    Uniprot ID   A0A5H6P140
Organism   Salmonella enterica subsp. enterica serovar Adjame strain 389598     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745681..756661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G9X25_RS03565 (G9X25_03565) - 746259..747266 (+) 1008 WP_000252198.1 DUF1202 domain-containing protein -
  G9X25_RS03570 (G9X25_03570) hemW 747357..748493 (-) 1137 WP_079943915.1 radical SAM family heme chaperone HemW -
  G9X25_RS03575 (G9X25_03575) - 748486..749079 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  G9X25_RS03580 (G9X25_03580) yggU 749087..749377 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  G9X25_RS03585 (G9X25_03585) - 749374..749940 (-) 567 WP_001094848.1 YggT family protein -
  G9X25_RS03590 (G9X25_03590) - 749959..750663 (-) 705 WP_175268139.1 YggS family pyridoxal phosphate-dependent enzyme -
  G9X25_RS03595 (G9X25_03595) pilT 750681..751661 (+) 981 WP_175268140.1 type IV pilus twitching motility protein PilT Machinery gene
  G9X25_RS03600 (G9X25_03600) - 751794..752480 (+) 687 WP_175268141.1 IclR family transcriptional regulator -
  G9X25_RS03605 (G9X25_03605) ruvX 752527..752943 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  G9X25_RS03610 (G9X25_03610) - 752943..753506 (-) 564 WP_001053171.1 YqgE/AlgH family protein -
  G9X25_RS03615 (G9X25_03615) gshB 753722..754669 (-) 948 WP_079841140.1 glutathione synthase -
  G9X25_RS03620 (G9X25_03620) rsmE 754689..755420 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  G9X25_RS03625 (G9X25_03625) endA 755497..756204 (-) 708 WP_175268142.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36073.26 Da        Isoelectric Point: 7.2930

>NTDB_id=454755 G9X25_RS03595 WP_175268140.1 750681..751661(+) (pilT) [Salmonella enterica subsp. enterica serovar Adjame strain 389598]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMEIAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPESCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTMAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=454755 G9X25_RS03595 WP_175268140.1 750681..751661(+) (pilT) [Salmonella enterica subsp. enterica serovar Adjame strain 389598]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGAGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTACTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGATGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5H6P140

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Acinetobacter baylyi ADP1

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.343

100

0.445
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.34

97.546

0.433
  pilT Legionella pneumophila strain ERS1305867

44.34

97.546

0.433
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.036

100

0.368