Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PMPD1_RS18515 Genome accession   NZ_CP054212
Coordinates   3840468..3841472 (-) Length   334 a.a.
NCBI ID   WP_173635426.1    Uniprot ID   A0A6M8UNR6
Organism   Paramixta manurensis strain PD-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3835468..3846472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMPD1_RS18485 (PMPD1_3651) - 3836160..3836663 (+) 504 WP_173635420.1 SprT family zinc-dependent metalloprotease -
  PMPD1_RS18490 (PMPD1_3652) endA 3836757..3837470 (+) 714 WP_173635421.1 deoxyribonuclease I -
  PMPD1_RS18495 (PMPD1_3653) rsmE 3837546..3838277 (+) 732 WP_173635422.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PMPD1_RS18500 (PMPD1_3654) gshB 3838381..3839328 (+) 948 WP_173635423.1 glutathione synthase -
  PMPD1_RS18505 (PMPD1_3655) - 3839443..3840006 (+) 564 WP_173635424.1 YqgE/AlgH family protein -
  PMPD1_RS18510 (PMPD1_3656) ruvX 3840006..3840437 (+) 432 WP_173635425.1 Holliday junction resolvase RuvX -
  PMPD1_RS18515 (PMPD1_3657) pilT 3840468..3841472 (-) 1005 WP_173635426.1 type IV pilus twitching motility protein PilT Machinery gene
  PMPD1_RS18520 (PMPD1_3658) - 3841489..3842202 (+) 714 WP_173635427.1 YggS family pyridoxal phosphate-dependent enzyme -
  PMPD1_RS18525 (PMPD1_3659) - 3842211..3842765 (+) 555 WP_173635428.1 YggT family protein -
  PMPD1_RS18530 (PMPD1_3660) rdgB 3842788..3843381 (+) 594 WP_173635429.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  PMPD1_RS18535 (PMPD1_3661) hemW 3843374..3844510 (+) 1137 WP_173635430.1 radical SAM family heme chaperone HemW -
  PMPD1_RS18540 (PMPD1_3662) - 3844707..3845549 (+) 843 WP_173635431.1 DUF3289 family protein -
  PMPD1_RS18545 (PMPD1_3663) - 3845546..3845998 (+) 453 WP_173635432.1 DUF943 family protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36032.39 Da        Isoelectric Point: 6.8762

>NTDB_id=451465 PMPD1_RS18515 WP_173635426.1 3840468..3841472(-) (pilT) [Paramixta manurensis strain PD-1]
MDIEEILALSVKHNAADLHLCSGHLPQWRRDGVLEAVPGQTVVDGALLESWARRWLTAAQQACFESSGQVDFAFSAAEGA
RVRASLFEQRAGLSLALRLIASTPMALEALQAPKMIEKLLRERDGLILVTGATGSGKSSTLAAMVAALNQRGARHILTLE
DPIEFVHHSQHSLIQQREIGQHCCSYQQGLRAALRADPDVILLGELRDIETIRLALTAAETGHLVLTTLHTRGATQAVER
LVDVFPGEEKSAVRSQLAGSLKAVIAQRLVPARQGGRVALFELLLNTPAVANLIREGKSHQLPAVLQTGAQAGMQSFEQC
LRERVSAGLVAEEV

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=451465 PMPD1_RS18515 WP_173635426.1 3840468..3841472(-) (pilT) [Paramixta manurensis strain PD-1]
ATGGATATAGAAGAAATCTTGGCCCTTAGTGTAAAGCATAATGCCGCCGATCTGCATCTTTGCAGCGGACATTTACCACA
ATGGCGGCGTGATGGCGTGCTGGAGGCGGTTCCCGGGCAAACGGTAGTTGATGGCGCGTTGCTGGAATCATGGGCTCGCC
GCTGGTTGACTGCGGCACAGCAGGCTTGCTTTGAATCAAGCGGTCAGGTGGATTTCGCTTTTTCCGCCGCAGAGGGGGCG
CGTGTGCGCGCCAGCCTGTTTGAGCAGCGCGCCGGGTTGTCGTTGGCGCTACGGCTGATCGCCAGCACGCCTATGGCTCT
TGAGGCTTTGCAGGCGCCAAAGATGATTGAGAAATTATTAAGAGAGCGTGACGGCTTAATTTTGGTGACTGGCGCGACCG
GGAGCGGCAAATCCTCCACGCTGGCGGCAATGGTGGCGGCGCTGAACCAGCGTGGCGCCCGGCATATTTTAACCCTCGAG
GATCCGATTGAGTTTGTCCATCACAGTCAGCATTCATTGATTCAGCAGCGTGAGATTGGGCAGCATTGTTGCTCGTATCA
GCAGGGTTTGCGTGCTGCGCTACGCGCGGATCCTGACGTGATTTTGCTTGGTGAATTACGCGATATTGAAACCATTCGTC
TGGCGCTGACCGCGGCGGAAACCGGCCATCTGGTATTAACCACCTTACATACCCGTGGCGCGACGCAGGCGGTTGAGCGG
TTGGTGGATGTCTTTCCCGGCGAAGAAAAGAGCGCGGTGCGCAGCCAATTGGCGGGGAGCCTGAAAGCGGTTATCGCCCA
GCGTTTAGTCCCGGCGAGGCAAGGTGGGCGCGTGGCATTGTTCGAACTGTTGCTGAATACGCCTGCGGTGGCGAATTTGA
TTCGCGAAGGGAAAAGTCACCAGTTACCGGCAGTGCTGCAAACCGGCGCGCAGGCGGGAATGCAGAGTTTCGAACAGTGT
TTGCGTGAGCGAGTCTCGGCGGGGTTGGTGGCGGAAGAGGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8UNR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.852

100

0.503
  pilT Vibrio cholerae strain A1552

49.852

100

0.503
  pilT Neisseria meningitidis 8013

49.112

100

0.497
  pilT Neisseria gonorrhoeae MS11

48.665

100

0.491
  pilT Pseudomonas stutzeri DSM 10701

46.847

99.701

0.467
  pilT Pseudomonas aeruginosa PAK

45.946

99.701

0.458
  pilT Legionella pneumophila strain ERS1305867

45.783

99.401

0.455
  pilT Legionella pneumophila strain Lp02

45.783

99.401

0.455
  pilT Acinetobacter baylyi ADP1

45.455

98.802

0.449
  pilT Acinetobacter nosocomialis M2

44.745

99.701

0.446
  pilT Acinetobacter baumannii D1279779

44.444

99.701

0.443
  pilT Acinetobacter baumannii strain A118

44.444

99.701

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

96.407

0.425
  pilU Vibrio cholerae strain A1552

40.356

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

37.725

100

0.377