Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HNY42_RS12395 Genome accession   NZ_CP053557
Coordinates   2363903..2364946 (-) Length   347 a.a.
NCBI ID   WP_131502735.1    Uniprot ID   -
Organism   Exiguobacterium sp. Helios     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358903..2369946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNY42_RS12375 (HNY42_12380) - 2359439..2361184 (-) 1746 WP_188004559.1 fimbrial assembly protein -
  HNY42_RS12380 (HNY42_12385) - 2361203..2361961 (-) 759 WP_188004560.1 A24 family peptidase -
  HNY42_RS12385 (HNY42_12390) - 2362018..2362542 (-) 525 WP_188004561.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HNY42_RS12390 (HNY42_12395) - 2362681..2363898 (-) 1218 WP_188004562.1 type II secretion system F family protein -
  HNY42_RS12395 (HNY42_12400) pilT 2363903..2364946 (-) 1044 WP_131502735.1 type IV pilus twitching motility protein PilT Machinery gene
  HNY42_RS12400 (HNY42_12405) - 2364943..2366607 (-) 1665 WP_188004563.1 GspE/PulE family protein -
  HNY42_RS12405 (HNY42_12410) - 2366616..2366930 (-) 315 WP_131502737.1 hypothetical protein -
  HNY42_RS12410 (HNY42_12415) - 2366958..2368319 (-) 1362 WP_251138590.1 G5 domain-containing protein -
  HNY42_RS12415 (HNY42_12420) - 2368495..2369019 (+) 525 WP_131502739.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HNY42_RS12420 (HNY42_12425) - 2368982..2369587 (+) 606 WP_131502740.1 type II secretion system protein J -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38134.92 Da        Isoelectric Point: 7.0662

>NTDB_id=445132 HNY42_RS12395 WP_131502735.1 2363903..2364946(-) (pilT) [Exiguobacterium sp. Helios]
MSTMERAQIEDILRSSKEKEASDVHLTAGSPPVFRINGTLTPFGTEKMKPVIIEGFVRSLITEEMFLQLKQDRDIDFSFG
VTGVARYRVNCFYQRGALSMAFRTIPTEVPTLDQLSLPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYKR
IITLEDPIEYLHSHQKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETISTAVTAAETGHLVFATVHTSTAA
STVSRIIDVFPSEQQDQIRAQLANVLAGVVSQRLMPRTDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYGM
QTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=445132 HNY42_RS12395 WP_131502735.1 2363903..2364946(-) (pilT) [Exiguobacterium sp. Helios]
ATGAGTACGATGGAGCGCGCCCAAATTGAAGATATCTTACGATCGTCGAAAGAAAAAGAAGCATCCGATGTCCACCTGAC
GGCCGGTTCACCGCCGGTCTTTCGCATCAACGGCACCTTGACACCATTCGGTACAGAAAAGATGAAACCGGTCATAATCG
AAGGGTTCGTCCGCTCCCTTATTACCGAGGAAATGTTTTTACAGTTAAAACAGGACCGGGATATTGATTTTTCATTTGGT
GTGACAGGAGTGGCACGCTACCGCGTCAATTGTTTTTATCAACGCGGGGCGTTGTCGATGGCCTTTCGGACGATTCCGAC
TGAAGTACCGACACTCGATCAATTGTCATTGCCGCCGGTCTTAAAACGTTTCCTGACGAAGCCGCACGGCTTGATTCTCG
TGACCGGTCCGACGGGTTCCGGGAAATCGACGACACTGGCAGCGATGATCAATGAAATCAACCAGACGATGTATAAGCGG
ATCATCACGCTCGAAGATCCGATCGAATATTTACACAGTCATCAGAAAAGTCTGATCGACCAGCGGGAAATCGGCATCGA
TGCCCCGCGTTTTGCGACCGGCTTACGGTCCGCCCTTCGCCAGGATCCAGATGTCATCCTGCTAGGTGAGATGCGGGATC
TTGAAACAATTTCAACAGCGGTGACGGCAGCAGAGACTGGTCATTTAGTTTTTGCGACGGTCCATACGTCGACGGCGGCA
TCGACGGTCAGTCGGATCATCGACGTCTTTCCATCCGAGCAACAAGACCAGATTCGGGCTCAACTCGCGAACGTTCTAGC
CGGTGTCGTCTCACAACGGCTGATGCCACGAACGGATGGAACCGGTCGTGTCGCAGCACTTGAAATCATGGTTGATACAC
CCGCCGTCTCAAACTTAATCCGGACCGGGAAAGAATATCAGATCCAATCGGTCCTACAGACAGGGAAGCAATACGGCATG
CAGACGATGCAGATGGCATTACAAGACTTAGTGAGTCGAGGTTTGATTCCAGCATCAGCCGCGACACCTTACATATCGGG
GTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.524

94.524

0.487
  pilT Neisseria gonorrhoeae MS11

49.701

96.254

0.478
  pilT Neisseria meningitidis 8013

49.701

96.254

0.478
  pilT Acinetobacter nosocomialis M2

48.657

96.542

0.47
  pilT Vibrio cholerae strain A1552

49.091

95.101

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

95.101

0.467
  pilT Acinetobacter baumannii D1279779

48.358

96.542

0.467
  pilT Acinetobacter baumannii strain A118

48.358

96.542

0.467
  pilT Pseudomonas aeruginosa PAK

48.348

95.965

0.464
  pilT Acinetobacter baylyi ADP1

48.171

94.524

0.455
  pilT Pseudomonas stutzeri DSM 10701

47.147

95.965

0.452
  pilT Legionella pneumophila strain Lp02

45.946

95.965

0.441
  pilT Legionella pneumophila strain ERS1305867

45.946

95.965

0.441
  pilU Acinetobacter baylyi ADP1

40

96.542

0.386
  pilU Pseudomonas stutzeri DSM 10701

40.426

94.813

0.383